Liquid column-chromatographic separation and determination of long-chain primary n-alkylamines (n-C10H21NH2 to n-C22H45NH2) after their fluorescence derivatization with salicylaldehyde diphenylboron chelate

Claas, K. E.; Hohaus, E.; Monien, H.

doi:10.1007/bf00983397

Cited by 6 publications

(2 citation statements)

References 5 publications

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“…RPLC methods for fatty amine analysis can be found in the literature, but the majority required a derivation step to make them UV detectable . A mass spectrometer is able to identify amines in their protonated state in the positive ion mode.…”

Section: Resultsmentioning

confidence: 99%

Development and evaluation of gas and liquid chromatographic methods for the analysis of fatty amines

et al. 2014

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In contrast to the plethora of publications on the separation of fatty acids, analogous studies involving fatty amines are scarce. A recently introduced ionic-liquid-based capillary column for GC was used to separate trifluoroacetylated fatty amines focusing on the analysis of a commercial sample. Using the ionic liquid column (isothermal mode at 200 °C) it was possible to separate linear primary fatty amines from C12 to C22 chain length in less 25 min with MS identification. The log of the amine retention factors are linearly related to the alkyl chain length with a methylene selectivity of 0.117 kcal/mol for the saturated amines and 0.128 kcal/mol for the mono-unsaturated amines. The sp2 selectivity for unsaturated fatty amines also could be calculated as 0.107 kcal/mol for the ionic liquid column. The commercial sample was quantified by GC with flame ionization detection (FID). An LC method also was developed with a reversed phase gradient separation using acetonitrile/formate buffer mobile phases and ESI-MS detection. Native amines could be detected and identified by their single ion monitoring chromatograms even when partial coelution was observed. The analysis of the commercial sample returned results coherent with those obtained by GC-FID and with the manufacturer's data.

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Section: Resultsmentioning

confidence: 99%

Development and evaluation of gas and liquid chromatographic methods for the analysis of fatty amines

et al. 2014

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“…Derivatization is usually introduced to facilitate detection and to increase the sensitivity of the analysis. Some typical compounds analyzed and the derivatizing agent used follow: aliphatic diamines treated with acetonylacetone (125); primary and secondary alkylamines and amino acids treated with succinimido phenylor naphthylcarbamate (126); pyrrolidine, piperidine, and morpholine treated with potassium l,2-naphthoquinone-4-sulfonate (127); primary amines in aqueous media (128a, 128b) and amino acids (128b) treated with naphthalene-2,3-dicarboxaldehyde in the presence of cyanide; primary alkylamines containing 10-22 carbon atoms treated with salicylaldehyde diphenylboron chelate (129); methyl-, dimethyl-, ethyl-, and diethylamine treated with 7-chloro-4-nitrobenzo-2-oxa-l,3-diazole (130); and trace aliphatic secondary amines treated with CS2 followed by Hg(II) (131).…”

Section: Aminesmentioning

confidence: 99%