Liquid chromatography with fluorescence detection of amoxicillin and ampicillin in feeds using pre-column derivatization

Rapid determination of amoxicillin in premixes by HPLC

“…In comparison with described method for determination of amoxicillin in feeds [15], developed HPLC method is very simple, rapid and enough sensitive for determination in premixes without derivatization step. Elimination of interfering compounds, without loss of target analyte, is achieved.…”

“…The method for determining amoxicillin in pharmaceuticals using sequential injection analysis (SIA) with a diode-array spectrophotometric detector was reported [8]. In the literature, there are many chromatographic methods with diode array and fluorimetric detection for the determination of penicillins in food of animal origin [9][10][11][12][13][14] and in feeds [15] or pharmaceutical dosage forms [16][17][18]. The UV detection is carried out directly and after pre-column derivatization with imidazole and mercuric chloride [19].…”

“…The UV detection is carried out directly and after pre-column derivatization with imidazole and mercuric chloride [19]. The fluorescence detection is carried out using post-column derivatization with fluorescamine [20,21] or pre-column derivatization with formaldehyde in acidic solution [15].…”

High Speed Liquid Chromatography for In-Process Control of Sultamicillin

“…Ampicillin and sulbactam determination has been performed, both in biological fluids and pharmaceuticals by different techniques, such as flow injection analysis, [7,8] polarography, [9,10] and spectrophotometry, [11][12][13] although HPLC [14][15][16][17][18][19][20][21][22][23][24][25] has chiefly been used. Furthermore, sultamicillin analysis has primarily been carried out in pharmaceutical formulations by HPLC, [26][27][28][29][30][31] according to recommendations by pharmacopoeias.…”

Quantitative analysis of amoxicillin, its major metabolites and ampicillin in eggs by liquid chromatography combined with electrospray ionization tandem mass spectrometry

“…Several methods have been published for the extraction of AMO, AMO metabolites and AMP from milk, plasma and tissues, including those based on HPLC-UV(Douša & Hosmanová, 2005;Ibrahim & Nasr, 2014), HPLC-FLD(Gamba & Dusi, 2003), HPLC-MS/MS(Colin, De Bock, T'jollyn, Boussery, & Van Bocxlaer, 2013), and UPLC-MS/MS(Reyns, Cherlet, De Baere, De Backer, & Croubels, 2008). However, there are no reports of assays capable of the simultaneous determination of the levels of AMO, AMO metabolites and AMP in egg samples.…”