Rapid determination of amoxicillin in premixes by HPLC

Douša, Michal; Hosmanova, Romana

doi:10.1016/j.jpba.2004.10.010

Cited by 78 publications

(45 citation statements)

References 21 publications

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“…It acts binding to penicillin binding protein (PBP-1A) located inside the bacterial cell wall, the amoxicillin acylate the penicillin-sensitive transpeptidase C-terminal domain by opening the lactam ring causing inactivation of the enzyme, prevents the formation of a cross-link of two linear peptidoglycan strands, inhibiting the third and last stage of bacterial cell wall synthesis, which is necessary for cell division and cell shape and other essential processes, producing as a consequence the lisis of the bacteria cells [37,[46][47][48][49]. The two major metabolites of amoxicillin are amoxicilloic acid and piperazine-2, 5-dione (diketopiperazine).…”

Section: Amoxicillinmentioning

confidence: 99%

Amoxicillin in the Aquatic Environment, Its Fate and Environmental Risk

Elizalde-Velázquez¹,

Gómez-Oliván²,

Galár-Martínez³

et al. 2016

Environmental Health Risk - Hazardous Factors to Living Species

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Amoxicillin is a broad-spectrum antibiotic widely used for treating both human and animal diseases, and it belongs to a group that are excreted unchanged within urine and faeces; therefore, it is possible to find traces of this drug or its degradation products in environmental water bodies. In water, it is rapidly degraded by biotic and abiotic factors, yielding different intermediate products; these are suspected of being more resistant to degradation, and potentially more toxic, than the parent compound. In the water bodies, these compounds may produce toxic effects on the aquatic organisms from different trophic levels and produce an ecological imbalance. Amoxicillin may bioaccumulate in fish muscle tissues, with the possibility of the occurrence of these drugs in food, leading to a passive consumption of this antibiotic resulting in undesirable effects on consumer health such as immunoallergic responses. However, the main problem related with the presence of this antimicrobial compounds in fish tissues is the possibility of inducing bacterial resistance genes. At present, the available scientific knowledge is less than what is needed to fully assess the risks that amoxicillin pose to the environment, and it is still necessary to conduct large amount of research works before a thorough understanding of this severe environmental issue.

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Section: Amoxicillinmentioning

confidence: 99%

Amoxicillin in the Aquatic Environment, Its Fate and Environmental Risk

Elizalde-Velázquez¹,

Gómez-Oliván²,

Galár-Martínez³

et al. 2016

Environmental Health Risk - Hazardous Factors to Living Species

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show abstract

“…An additional aim of derivatization is the introduction of chemical moieties that improve detection by liquid chromatography-fluorescence [5,9]. Methods involving high-performance liquid chromatography (HPLC) using ultraviolet (UV) [10], mass spectrometry (MS) [11], or diode array detection (DAD) [12] were reported for the determination of amoxicillin and another penicillin drugs in milk [13], fish [7], urine [14], human plasma [12], and animal plasma, e.g., rats [15], mouse [16], bovine [17], or pigs [18]. This paper describes a sensitive method for the determination of amoxicillin in poultry plasma.…”

Section: Introductionmentioning

confidence: 99%

Determination of amoxicillin in poultry plasma by high-performance liquid chromatography after formaldehyde derivation

Krasucka¹,

Kowalski²,

Osypiuk³

et al. 2015

Acta Chromatographica

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Summary.A high-performance liquid chromatographic (HPLC) method with fluorescence detection after precolumn formaldehyde derivation was developed to detect concentrations of amoxicillin (AMX) in poultry plasma. Proteins in plasma samples spiked with AMX were precipitated with a phosphate buffer and trichloroacetic acid. After precolumn treatment of the extraction product of AMX with formaldehyde under acidic and heating conditions, HPLC analysis with fluorescence (FL) detection at an excitation wavelength of 355 nm and an emission wavelength of 450 nm was performed. A mobile phase comprising acetonitrile and a buffer solution (0.05 M KH2PO4 pH = 5.6), which yielded AMX retention time 8.58 min, was suitable for detection of AMX. The calculated standard curve of the reaction product was linear, and the correlation coefficient was greater than 0.999. The limit of detection and quantification, the accuracy, and the precision were evaluated. Recoveries of spiked amoxicillin were >92%, with a coefficient of variation in the range of 0.35-0.89%. This method has been successfully applied to a pharmacokinetic study after oral administration of amoxicillin to poultry.

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“…Later HPLC methods using reversed-phase and UV detection, and column switching or amperometric detection [9][10][11][12][13][14][15] has been developed. In order to enhance selectivity and sensitivity, some methods with fluorescence detection are carried out using post-columns [16][17] or pre-columns [19].…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a method to determine amoxicillin in physiological fluids using micellar liquid chromatography

Rambla-Alegre

Carda‐Broch

Romero³

2008

J of Separation Science

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Abbreviations MLC micellar liquid chromatographyFDA food and drug analysis 2 Abstract A simple and robust method was developed for the routine identification and quantification of amoxicillin by micellar liquid chromatography. Amoxicillin, a β-lactamase inhibitor, is one of the most commonly prescribed drugs in the treatment of urine and skin structure infections. In this work, amoxicillin was determined in urine samples without any pretreatment step in a phenyl column using a micellar mobile phase of 0.10 M sodium dodecyl sulfate and 4% butanol at pH 3. A UV detection set at 210 nm was used. Amoxicillin is eluted at 5.1 min with no interference by the protein band or endogenous compounds. Linearities (r > 0.9998), intra-and inter-day precisions were determined (RSD (%) 0.4-2.7% and 0.3-5%, respectively in micellar media, and 0.14-2.6% and 0.13-6%, respectively in urine), and robustness was studied in the validation of the method. LOD and LOQ were 0.06 and 0.4 µg/mL in micellar media and 0.11 and 0.4 µg/mL in urine, respectively. Recoveries in the urine matrix were in the range of 95-110%. The validated method proved to be reliable and sensitive for the determination of amoxicillin in urine samples.

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Rapid determination of amoxicillin in premixes by HPLC

Cited by 78 publications

References 21 publications

Amoxicillin in the Aquatic Environment, Its Fate and Environmental Risk

Amoxicillin in the Aquatic Environment, Its Fate and Environmental Risk

Determination of amoxicillin in poultry plasma by high-performance liquid chromatography after formaldehyde derivation

Development and validation of a method to determine amoxicillin in physiological fluids using micellar liquid chromatography

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