Quantitative analysis of amoxicillin, its major metabolites and ampicillin in eggs by liquid chromatography combined with electrospray ionization tandem mass spectrometry

Sun, Lirui; Jia, Longfei; Xie, Xiulan; Xie, Kaizhou; Wang, Jianfeng; Liu, Jianyu; Cui, Lulu; Zhang, Genxi; Dai, Guojun; Wang, Jinyu

doi:10.1016/j.foodchem.2015.07.028

Cited by 62 publications

(27 citation statements)

References 16 publications

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“…The method met the requirements established by the EU (Commission decision 657/EC, ) for detecting AMO, AMA and DIKETO in commercial eggs according to a previous study by Sun et al . (Sun et al ., ), including the linearity, recovery, precision, quantification limit (LOQ), detection limit (LOD) and specificity. The recoveries of AMO, AMA and DIKETO ranged from 81.02% to 91.25% in the albumen, 80.97% to 91.59% in the yolk and 80.34% to 92.17% in the whole egg at adding concentrations of 5.0, 10.0 and 20.0 μg/kg.…”

Section: Methodsmentioning

confidence: 99%

“…The conditions for sample preparation were identical to those used in our previous study (Sun et al, 2016). Briefly, albumen, yolk or whole egg samples were homogenized, and 5 g of egg homogenate was accurately weighed and transferred into a 50-mL polypropylene centrifuge tube containing 100 lL PV (7.5 lg/mL); the mixture was then vortexed for 10 s. Acetonitrile (10 mL) was added to the homogenized mixture, followed by vortexing for 60 s and ultrasonic oscillation for 15 min at room temperature.…”

Section: Sample Extraction and Clean-upmentioning

confidence: 99%

“…AMO is widely used in veterinary medicine to prevent and treat bacterial infections in animals due to its broad-spectrum antibacterial effect and low cost. It has previously been used to treat primary respiratory conditions, skin infections, urethral infections and bacterial overgrowth in the gastrointestinal tract (Sun et al, 2016). According to the FDA (2015), AMO was also approved for offlabel use in both nonfood product animals and food product animals when the health of an animal is threatened.…”

Section: Introductionmentioning

confidence: 99%

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Residue depletion of amoxicillin and its major metabolites in eggs

Liu

Pang

Xie

et al. 2016

Vet Pharm & Therapeutics

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The depletion of amoxicillin (AMO) and its major metabolites, amoxicilloic acid (AMA) and amoxicillin-diketopiperazine-2',5'-dione (DIKETO) in the albumen, yolk and whole egg was studied after the oral dose of AMO (25 and 50 mg/kg body weight) to laying hens once per day for five consecutive days. Egg samples were prepared by a simple liquid-liquid extraction procedure with acetonitrile and saturated methylene chloride and analysed using liquid chromatography-tandem mass spectrometry. The results showed that AMO, AMA and DIKETO residues were mainly distributed in the yolk, where particularly high concentrations of AMO and DIKETO were found, whereas the albumen contained high concentrations of AMA. This distribution suggested that AMO and DIKETO were depleted slowly in yolk, whereas AMA was depleted slowly in albumen. The amount of AMO residue positively correlated with the dose, and the theoretical withdrawal times, which were calculated based on the residue level falling below a safe limit, were 5.21 and 7.67 days at AMO doses of 25 and 50 mg/kg, respectively. Moreover, the theoretical withdrawal times for all residues in the whole egg were 8.00 and 9.11 days at doses of 25 and 50 mg/kg, respectively. Our findings suggested that 9 days was an appropriate withdrawal time for the use of AMO in laying hens.

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Section: Methodsmentioning

confidence: 99%

Section: Sample Extraction and Clean-upmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Residue depletion of amoxicillin and its major metabolites in eggs

Liu

Pang

Xie

et al. 2016

Vet Pharm & Therapeutics

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“…The aim of the research was the development of a method based on micellar liquid chromatography (MLC) to determine MBZ in milk and other dairy products such as curd, butter, and cheese to check the absence of residues in the food and ensure consumer protection, and farming waste (urine and dung), since its presence in excretions is proof the drug has been administered to the bovine animal. MLC allows direct injection of samples into the chromatographic system without any pretreatment; using sodium dodecyl sulfate (SDS) as a surfactant in mobile phase makes the method rapid, sensitive, cost effective, and eco-friendly, as well as serves as an alternative to conventional HPLC system [20][21][22][23]. These are some of the main reasons behind selecting MLC to carry out the present research work.…”

Section: Introductionmentioning

confidence: 99%

Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals

et al. 2020

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Mebendazole is an anthelmintic drug used in cattle production. However, residues may occur in produced food and in excretions, jeopardizing population health. A method based on micellar liquid chromatography (MLC) was developed to determine mebendazole in dairy products (milk, cheese, butter, and curd) and nitrogenous waste (urine and dung) from bovine animals. Sample treatment was expedited to simple dilution or solid-to-liquid extraction, followed by filtration and direct injection of the obtained solution. The analyte was resolved from matrix compounds in less than 8 min, using a C18 column and a mobile phase made up of 0.15 M sodium dodecyl sulfate (SDS)-6% 1-pentanol phosphate buffered at pH 7, and running at 1 mL/min under isocratic mode. Detection was performed by absorbance at 292 nm. The procedure was validated according to the guidelines of the EU Commission Decision 2002/657/EC in terms of: specificity, method calibration range (from the limit of quantification to 25-50 ppm), sensitivity (limit of detection 0.1-0.2 ppm; limit of quantification, 0.3-0.6 ppm), trueness (92.5-102.3%), precision (<7.5%, expressed at RSD), robustness, and stability. The method is reliable, sensitive, easy-to-handle, eco-friendly, safe, inexpensive, and provides a high sample-throughput. Therefore, it is useful for routine analysis as a screening or quantification method in a laboratory for drug-residue control.

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“…Many analytical techniques have been reported for the determination of AMX such as spectrometry, chromatography and chemiluminescence . Furthermore, most of the mentioned methods are expensive, needing complex pretreatments, consuming large samples volumes and time.…”

Section: Introductionmentioning

confidence: 99%

Chronoamperometric Detection of Amoxicillin at Graphite Electrode using Chelate Effect of Copper(II) Ions : Application in Human Blood and Pharmaceutical Tablets

et al. 2019

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A novel electrochemical method is suggested for indirect chronoamperometric determination of amoxicillin (AMX) in the presence of copper ions Cu (II) at carbon paste electrode (CPE). The method is based on the interaction between the β-lactam antibiotic and copper ions. This interaction was firstly studied by square wave voltammetry (SWV), where the peak corresponding to the oxidation of AMX at 0.7 V decreases significantly in the presence of copper ions. The reaction product is characterized by UV-visible spectrophotometry and Infrared spectroscopy (IR). Moreover, when AMX was added to supporting electrolyte containing copper ions, the chronoamperomet-ric current of the Cu(II)/Cu(I) redox system was increased. Hence, this increase in the current was used for indirect determination of amoxicillin. By selecting an appropriate concentration of copper (II), the calibration curve for AMX was established in the concentration range of 1.95 × 10 À 7 to 1.46 × 10 À 5 mol L À 1 . The detection limit was found to be 8.84 × 10 À 8 mol L À 1 . The influence of different organic compounds on the AMX determination and the effect of coexisting of other metal ions in the electrolytic solution were studied. The applicability of the proposed method was tested in human blood and pharmaceutical tablets.

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Quantitative analysis of amoxicillin, its major metabolites and ampicillin in eggs by liquid chromatography combined with electrospray ionization tandem mass spectrometry

Cited by 62 publications

References 16 publications

Residue depletion of amoxicillin and its major metabolites in eggs

Residue depletion of amoxicillin and its major metabolites in eggs

Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals

Chronoamperometric Detection of Amoxicillin at Graphite Electrode using Chelate Effect of Copper(II) Ions : Application in Human Blood and Pharmaceutical Tablets

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