Liquid Chromatography–Single-Quadrupole Mass Spectrometry as a Responsive Tool for Determination of Biogenic Amines in Ready-to-Eat Baby Foods

Abstract

Baby food has never been the object of biogenic amine profiling. The aim of this study was to develop a highly sensitive method for analysis of biogenic amines in ready-to-eat baby foods. The principle of the developed method involves high-performance liquid chromatography coupled to single-quadrupole mass spectrometry (HPLC–APCI–MS) of dansyl derivatives, presented also in comparison with common diode array and fluorescence detection systems. The confirmation of correct identification of derivatives was performed by in-source fragmentation of the product ion at 170 m/z, performed only in one MS analyzer. The method was used to identify the amine profile and quantify the putrescine, cadaverine, histamine, tyramine, spermidine, and spermine content in 68 ready-to-eat baby foods. The limits of detection and quantification were in the range of 0.07–1.67 and 0.2–5.0 ng mL− 1. The method enabled quantification of amines at ng/g level in almost all analyzed samples, without any preconcentration step. Amine recoveries of 86.0–105.2% were obtained with RSD ≤ 9.7%. The developed method could be used for quantification of the most frequently occurring BAs in foods including vegetables, fish, meat, or fruit at previously undetectable concentration levels, making the method multimatrix applicable and highly-sensitive.Electronic supplementary materialThe online version of this article (10.1007/s10337-018-3527-z) contains supplementary material, which is available to authorized users.

Cadaverine as a Potential Spoilage Indicator in Skin-Packed Beef and Modified-Atmosphere-Packed Beef

“…The sample was then centrifuged (4500 rpm, 60 min, 4 • C). Cadaverine extraction was performed using perchloric acid, as previously outlined [12,21,22]. Subsequent purification used Oasis WCX (60 mg, 3 cc) (Waters Corp., Milford, MA, USA) and Strata XL-CW (100 mg, 6 cc) (Phenomenex, Torrence, CA, USA) ion-exchange columns for MB and EB, respectively [23], followed by chromatographic separation using a reverse-phase column (Kinetex 2.6 µm C18 100A, 100 × 3 mm) (Phenomenex) with heptafluorobutyric acid as an ion-pairing agent [24,25].…”

Current Trends in Detection of Histamine in Food and Beverages

“…An IBCF (isobutyl chloroformate) derivatization and microextraction method followed by gas chromatography–mass spectrometry (GC-MS) was applied to extract four biogenic amines, including histamine, from Iranian Lighvan cheese samples with a detection limit of 10 ng/g for histamine . Simultaneous determination of six derivatized biogenic amines from baby food is easily achieved (with a detection limit of 0.07 ng/mL for histamine) using HPLC coupled with single-quadrupole mass spectrometry (HPLC-APCI-MS) . Ultra-HPLC coupled with a single-stage high-resolution mass spectrometer (Orbitrap) allowed the detection of 10 biogenic amines with a limit of quantification for histamine of 5 mg/kg in fish meat …”

“…77 Simultaneous determination of six derivatized biogenic amines from baby food is easily achieved (with a detection limit of 0.07 ng/mL for histamine) using HPLC coupled with single-quadrupole mass spectrometry (HPLC-APCI-MS). 78 Ultra-HPLC coupled with a single-stage high-resolution mass spectrometer (Orbitrap) allowed the detection of 10 biogenic amines with a limit of quantification for histamine of 5 mg/kg in fish meat. 79 2.3.…”

Critical review on microextraction techniques used in determination of histamine in food samples

“…In particular, the High-Performance Liquid Chromatography (HPLC) separation combined with different kinds of detectors play the most important role in the histamine quantification. These detection methods are Molecular Fluorescence Spectroscopy [24,25], Ultraviolet Absorption Spectroscopy [26,27], diode array UV-Vis, Mass Spectrometry (MS or MS-MS) [28,29], Thin Layer Chromatography (TLC) [30,31], colorimetry [32], fluorimetry [33], Ion Mobility Spectrometry (IMS) [34], Filter Paper Electrophoresis [35], commercially available histamine test kits based on enzymatic methods [36,37], Gas Chromatography-Flame Ionization and Detector (GC-FID) Gas Chromatography-Mass Spectrometry (GS-MS) [38].…”