Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry and Rapid Evaporative Ionization Mass Spectrometry Were Used to Develop a Lamb Authentication Method: A Preliminary Study

Wang, Jishi; Xu, Lei; Xu, Zhenzhen; Wang, Yanyun; Niu, Chune; Yang, Shi‐Yao

doi:10.3390/foods9121723

Cited by 14 publications

(7 citation statements)

References 41 publications

(40 reference statements)

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“…In this regard, there has been a growing research interest in meat authentication in terms of the geographical and dietary background of animals, and several analytical methods have been proposed for that purpose [ 4 ]. Those methods are based on more or less complicated laboratory techniques intended to identify reliable predictors such as fatty acids (FA), volatile compounds, stable isotopes or a variety of metabolites in meat [ 5 , 6 , 7 , 8 , 9 ]. The FA profile of intramuscular fat (IMF) has proven to efficiently discriminate between diets fed to fattening lambs [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Odd- and Branched-Chain Fatty Acids in Lamb Meat as Potential Indicators of Fattening Diet Characteristics

Gómez‐Cortés

Requena

Rueda

et al. 2021

Foods

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There is a growing interest of researchers in meat authentication in terms of geographical and dietary background of animals, and several analytical methods have been proposed for the purpose of investigating this. We hypothesized that the odd- and branched-chain fatty acid (OBCFA) profile in intramuscular fat (IMF) might suffice to distinguish lamb meat entering the food chain supply on the basis of the type of diet fed to lambs during the fattening period. A total of 30 individual OBCFA profiles, quantified by gas chromatography, of IMF of Manchego lambs were used. During the fattening period (42 days), the lambs were fed three diets differing in concentrate composition: (i) Control, concentrate typical of commercial fattening rations, rich in starch and based on cereals and soybean meal; (ii) Camelina, similar to Control but replacing 50% of the soybean meal with camelina meal; and (iii) Fibrous, concentrate rich in neutral detergent fiber (NDF), based on fibrous by-products and not including cereals nor soybean meal. The OBCFA were grouped into three classes (linear odd, iso and anteiso fatty acids) and were then submitted to a linear discriminant analysis, using the feeding treatments as grouping variable and the OBCFA class contents in IMF as quantitative variables. The results suggested that a high NDF to starch ratio of the concentrate, being the lowest for Control (CON) treatment and the highest for Fibrous (FIB) treatment, would be negatively related to the odd/anteiso ratio and positively related to the iso/(anteiso+odd) FA ratio in IMF. Determination of OBCFA profile in lamb meat would be useful to monitor the feeding regime (starch- or NDF-rich) of lambs entering the food chain supply.

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Section: Introductionmentioning

confidence: 99%

Odd- and Branched-Chain Fatty Acids in Lamb Meat as Potential Indicators of Fattening Diet Characteristics

Gómez‐Cortés

Requena

Rueda

et al. 2021

Foods

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“…Apart from spectrophotometry, chromatography is also used in metabolic analysis. In metabolomic analysis, the use of LC-MS is critical, and one example is the use of ultra-high performance liquid chromatography quadrupole time-of-flight with rapid evaporative ionization mass spectrometry, which can provide metabolites in meat ( Wang et al, 2020 ). The workflow of metabolomics analysis in meat samples can be seen in Fig.…”

Section: Authentication Of Meat Product Using Metabolomics and Lipido...mentioning

confidence: 99%

“…The metabolite profile of pork differs greatly from that of beef, hence the latter’s metabolite profile could be used as a baseline for determining the meat’s authenticity. Metabolomic and lipidomic can also distinguish pork mixture in mutton and chicken by looking at the different metabolites and lipid profiles ( Wang et al, 2020 ). Lipidomics could be another way to look into the existence of pork mixture in meat products ( Castro-Puyana et al, 2017 ; Yang et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Possibilities of Liquid Chromatography Mass Spectrometry (LC-MS)-Based Metabolomics and Lipidomics in the Authentication of Meat Products: A Mini Review

Harlina¹,

Maritha²,

Musfiroh³

et al. 2022

Food Sci Anim Resour

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The liquid chromatography mass spectrometry (LC-MS)-based metabolomic and lipidomic methodology has great sensitivity and can describe the fingerprint of metabolites and lipids in pork and beef. This approach is commonly used to identify and characterize small molecules such as metabolites and lipids, in meat products with high accuracy. Since the metabolites and lipids can be used as markers for many properties of a food, they can provide further evidence of the foods authenticity claim. Chromatography coupled to mass spectrometry is used to separate lipids and metabolites from meat samples. The research data usually is compared to lipid and metabolite databases and evaluated using multivariate statistics. LC-MS instruments directly connected to the metabolite and lipid databases software can be used to assess the authenticity of meat products. LC-MS has good selectivity and sensitivity for metabolomic and lipidomic analysis. This review highlighted the combination of metabolomics and lipidomics can be used as a reference for analyzing authentication meat products.

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“…In order to pursue economic interests, illegal producers often use mutton to replace lamb meat. The high-throughput metabolomics approach combined with multivariate data analysis has been proven to distinguish lamb from mutton effectively [145].…”

Section: Species Originmentioning

confidence: 99%

Current State of Metabolomics Research in Meat Quality Analysis and Authentication

Tao

Chen

Xie

et al. 2021

Foods

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In the past decades, as an emerging omic, metabolomics has been widely used in meat science research, showing promise in meat quality analysis and meat authentication. This review first provides a brief overview of the concept, analytical techniques, and analysis workflow of metabolomics. Additionally, the metabolomics research in quality analysis and authentication of meat is comprehensively described. Finally, the limitations, challenges, and future trends of metabolomics application in meat quality analysis and meat authentication are critically discussed. We hope to provide valuable insights for further research in meat quality.

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Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry and Rapid Evaporative Ionization Mass Spectrometry Were Used to Develop a Lamb Authentication Method: A Preliminary Study

Cited by 14 publications

References 41 publications

Odd- and Branched-Chain Fatty Acids in Lamb Meat as Potential Indicators of Fattening Diet Characteristics

Odd- and Branched-Chain Fatty Acids in Lamb Meat as Potential Indicators of Fattening Diet Characteristics

Possibilities of Liquid Chromatography Mass Spectrometry (LC-MS)-Based Metabolomics and Lipidomics in the Authentication of Meat Products: A Mini Review

Current State of Metabolomics Research in Meat Quality Analysis and Authentication

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