Liquid chromatography–electrospray mass spectrometric analysis of curcuminoids and sesquiterpenoids in turmeric (Curcuma longa)

He, Xiaoxiao; Lin, Long-Ze; Lian, Li-zhi; Lindenmaier, Michael

doi:10.1016/s0021-9673(98)00540-8

Cited by 128 publications

(68 citation statements)

References 14 publications

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“…Os produtos principais do açafrão são a curcumina e o óleo essencial os quais são usados como condimento, corante natural, e aplicações farmacológicas 14 . Contudo, faz-se interessante a possibilidade de aproveitamento do resíduo da extração de óleos essenciais e corantes como matéria-prima para obtenção do amido.…”

Section: Resultsunclassified

Análise da forma e tamanho de grânulos de amidos de diferentes fontes botânicas

Leonel¹

2007

Ciênc. Tecnol. Aliment.

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*A quem a correspondência deve ser enviada ResumoNeste trabalho, objetivou-se avaliar a forma e tamanho de grânulos de amido de diferentes fontes botânicas, visando fornecer informações que contribuam para aplicabilidade destas como matérias-primas amiláceas. As féculas foram obtidas a partir do processamento das tuberosas em planta piloto de extração. Para a análise da forma dos grânulos utilizou-se o microscópio eletrônico de varredura, com as amostras diluídas em álcool etílico e metalizadas com ouro. A análise do tamanho dos grânulos foi realizada em microscópio ótico, sendo as amostras diluídas em solução de água e glicerina. Foram realizadas 500 determinações das medidas de tamanho (diâmetro menor, diâmetro maior e diferenças entre diâmetros) por fonte botânica. Os resultados obtidos mostraram diferentes formas para os grânulos de amidos e das tuberosas estudadas. Os grânulos de amido de menor tamanho foram os de taioba (Xanthosoma sp.) 12,87 m, e os de maior tamanho os de biri (Canna edulis) 59,61 m. Observou-se distribuição bastante regular para os grânulos de amido de ahipa (Pachyrhizus ahipa). Palavras-chave: amido; raízes; microscopia. AbstractAn analysis was made of the shape and size of starch grains from different botanical species, aiming to improve the potential applicability of these species as starch raw materials. The starches were obtained from the processing of tubers in a pilot extraction plant. The shape of the starch grains was analyzed by scanning electron microscopy (SEM), to which end the samples were mounted on SEM stubs and coated with gold. The size of the starch grains was determined by optical microscopy, for which the samples were diluted in a water and glycerin solution. The sizes of 500 grains of each botanical species were measured to determine the largest and smallest diameters and the difference between diameters. The results revealed different starch grain shapes and sizes, with Xanthosoma sp showing the smallest (12.87 m) and Canna edulis the largest grain size (56.61 m). The yam bean tuber Pachyrhizus ahipa showed the most uniform starch grain distribution. Keywords: starch; roots; microscopy. Análise da forma e tamanho de grânulos de amidos de diferentes fontes botânicas Analysis of the shape and size of starch grains from different botanical speciesMagali LEONEL 1 * IntroduçãoAs indústrias de alimentos e os produtores agrícolas estão interessados na identificação e no desenvolvimento de espécies que produzam amidos nativos com características especiais. Estes amidos poderiam substituir amidos modificados quimicamente ou abrir novos mercados para amidos 1 .O tamanho e a forma de grânulos de amido estão entre os fatores de importância na determinação de usos potenciais de amidos. Por exemplo, grânulos pequenos (2,0 m) podem ser usados como substitutos de gordura devido ao tamanho ser semelhante ao dos lipídeos. Outras aplicações, nas quais o tamanho dos grânulos é importante, é a produção de filmes plásticos biodegradáveis e de papéis para fax.O grânulo de amido tem sido ...

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Section: Resultsunclassified

Análise da forma e tamanho de grânulos de amidos de diferentes fontes botânicas

Leonel¹

2007

Ciênc. Tecnol. Aliment.

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“…Protonated ions are usually generated by ESI-MS in positive ion mode (He et al 2013). Identification of compounds in botanical extracts is established by a combination of useful online system liquid chromatography (LC) photodiode array UV-vis detection and electrospray ionisation mass spectrometry (ESI-MS) (He et al 1998;Cubbon et al 2010). Swertiamain, Gentiopicrin and Mangiferin were identified by TLC in Swertia chirayita and its content was determined by HPLC (Yuan-can et al 2010).…”

Section: Introductionmentioning

confidence: 99%

LC-ESI/MS determination of xanthone and secoiridoid glycosides from in vitro regenerated and in vivo Swertia chirayita

Kumar

Chandra²

2014

Physiol Mol Biol Plants

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A rapid analytical method has been developed to determine xanthone and secoiridoid glycoside in in vitro and in vivo Swertia chirayita extracts. Ultra performance liquid chromatography-electrospray ionization mass spectrometry (LC-ESI/MS) was applied and validated for the analysis of xanthone and secoiridoid glycoside a potential active component isolated from methanolic extracts of in vitro and in vivo Swertia chirayita plantlets. Chromatographic separation was achieved on a RP-C18 column using gradient elution. Mangiferin (Xanthone), Amarogentin and Swertiamarin (Secoiridoid glycosides) were identified in both the extracts. In the LC/ESI-MS spectra, major [M+H] + and [M+Na] + ions were observed in positive ion mode and provided molecular mass information. An ultra-performance liquid-chromatography in combination with electrospray ionization tandem mass spectrometry involving metal cationisation was successfully utilized for the rapid identification of xanthone and secoiridoid glycosides. This method is suitable for the routine analysis, as well as for the separation and identification of known and novel secoiridoid glycoside and xanthone.

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“…Curcumin has recently attracted increased attention because of s potent anti-leishmanial properties 6 , and ability to reduce alcohol-induced liver disease 7 and Alzheimer's disease 8 . Several methods have been developed to separate the curcuminoids: thin-layer chromatography (TLC) 9 , supercritical fluid chromatography (SFC) 10 , gas chromatography (GC) 11 , high performance liquid chromatography (HPLC) 12,13 , and HPLC-mass spectrometry (LC-MS) 11,14 .…”

Section: Introductionmentioning

confidence: 99%

Potential of Ethanolic Extract of Ficus Benghalensis on Open Wounds and Inflammation

Charde¹,

Dhongade²,

Charde³

et al. 2012

Int Jour of Pharmc Res

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AbstractsThe present study deals with evaluation of antioxidant, wound healing and anti-inflammatory activity of ethanolic extract of Curcuma longa Linn rhizomes. The ethanolic extract prepared by maceration technique was subjected to screen for antioxidant activity using DPPH radical scavenging method and wound healing activity using incision, excision, histopathological and dead space wound model and the study was supported with evaluation of granuloma tissue to estimate hydroxyproline content and histopathological evaluation. The anti-inflammatory study was carried out by using carageenan induced rat paw odema method. The tested extract of different dilutions in range 200µg/ml to 1000 µg/ml shows activity in range of 9.34% to 18.55%. Significant increase in wound closure rate, skin breaking strength, granuloma breaking strength was observed. The hydroxyproline content was also increased with decrease in scar area. The initial healing action might be due to increased collagen deposition and better alignment, with the obtained results it can be concluded that Curcuma longa extract has significant wound healing activity and initial healing may be due to presence of some terpenoids and antimicrobial agents. The extract shows prominent anti-inflammatory activity as compared to that of standard (Ibuprofen gel). The extract shows good anti-inflammatory activity on carageenan induced rat paw odema method.

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Liquid chromatography–electrospray mass spectrometric analysis of curcuminoids and sesquiterpenoids in turmeric (Curcuma longa)

Cited by 128 publications

References 14 publications

Análise da forma e tamanho de grânulos de amidos de diferentes fontes botânicas

Análise da forma e tamanho de grânulos de amidos de diferentes fontes botânicas

LC-ESI/MS determination of xanthone and secoiridoid glycosides from in vitro regenerated and in vivo Swertia chirayita

Potential of Ethanolic Extract of Ficus Benghalensis on Open Wounds and Inflammation

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