Liquid chromatographic determination of uric acid and ascorbic acid in rat retinae after ophthalmic artery and optic nerve ligation

Iriyama, Keiji; Yoshiura, Masahiko; Iwamoto, Takeo; Kamada, Yoshio; Nozawa, H; Mizuno, A; Matsuzaki, Hiroshi

doi:10.3109/02713688608996369

Cited by 3 publications

(2 citation statements)

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“…In addition to ascorbic acid, other major antioxidants such as uric acid and tyrosine have been found in increased concentrations in the aqueous humour as compared to the serum [21]. The concentrations of these antioxidants in the eye are influenced by numerous factors; therefore, in the case of experimental injury or dysfunction uric acid concentrations may rise, whereas ascorbic acid concentrations may fall [15]. Uric acid and tyrosine can counteract the oxidative capacity of H 2 O 2 only indirectly [27].…”

Section: Discussionmentioning

confidence: 96%

Prooxidant–antioxidant balance, peroxide and catalase activity in the aqueous humour and serum of patients with exfoliation syndrome or exfoliative glaucoma

Κολιάκος

Befani

Mikropoulos

et al. 2008

Graefes Arch Clin Exp Ophthalmol

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Section: Discussionmentioning

confidence: 96%

Prooxidant–antioxidant balance, peroxide and catalase activity in the aqueous humour and serum of patients with exfoliation syndrome or exfoliative glaucoma

Κολιάκος

Befani

Mikropoulos

et al. 2008

Graefes Arch Clin Exp Ophthalmol

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“…However, previous studi es on uric acid concentrations in monk eys and rats (18)(19)(20) indicate that the concentrat ion of uric acid in the aqueous humor and in the retina depends on local synthesis, rather than on a systemic increase in the con centration . Our findings of no change in uric acid concentrations in the vitreous humor after hypoxemia indicate that under these conditions no uric acid is form ed.…”

Section: Statist Icsmentioning

confidence: 98%

Changes in Oxypurine Concentrations in Vitreous Humor of Pigs during Hypoxemia and Post-Mortem

et al. 1990

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MATERIAlS AND METHODSprolonged disease process, which ma y include lon g-standing hypo xia (2, 3). During tissue hypox ia, elevated hypo xanthine levels have been found in plasma (4)(5)(6), CSF (7,8), and urine (9). We have also measured the hypoxanthine levels in vitreous humor post-mo rtem, and documented high levels when death was preceded by resp iratory failure and hypo xem ia ( 10, II ). Re cently, we reported high levels of hypoxanthine in the vitreous humor of victims of SIDS (\2), and therefore concluded that hypoxemia precedes death in SIDS .In our present study, we report how three degrees of hypoxemia in young pigs influence the level of hyp oxanthine, xanthine, and uri c acid in the vitreous humor. Furthermore, we in vestigated the time factor in volved in hypoxanthine accumulation in the vitreo us humor during hypo xemia. We also report how hypoxanthine concentrat ion s in the vitreo us humor change during th e first 24 h post-mortem . We found this important, in asmuch as the tim e from death to sam ple collection could ha ve influenced the concentrations of hypoxanthine in the vitreous humor that we have rep ort ed from our SIDS cases.Approval. The local hospital's ethical committee for animal studies approved thes e experiments.Animal m odel. The animal model was similar to that previou sly described (13). T wenty-two young pigs of either sex (the males had been castrated short ly after birth) weighing 17-22 kg (mea n 18.1 kg) were used . They were anesthetized with an intraperitoneal injection of sod ium pentobarbital (30 mg/kg), An i.v.I ine was establi shed through an ear vein , and a continuous infusion of Ringer acetate (\ 0 mL/kg/h) was given during the experim ent. A furth er 100 mg of sodium pentobarbital was given i.v. if necessary every 30 min. A cuffed tube was placed in the trachea via a tracheostomy. Artificial ventilation was given by a ventilator (Servo-ventilator 900B , Elema-Schenander, Stockholm, Sweden). Ventilation rate and tid al volume were adjusted until arterial CO 2 ten sion ranged betwe en 4 and 5.3 kPa (30-40 mm Hg). No further adjustments of the ventilator were done during the hypo xemic period.Polyeth ylene catheters were inserted into a fem oral artery and a femoral vein. The arterial catheter was used for measuring blo od gases. The veno us catheter was used for giving Ringer acetate plus additional sodium pentobarbital.After surge ry, the pigs were left for a stabilizing period of at least I h before basal concentrations were measured, and then divided into three hypoxemic and one control group. Seven animals were made hypoxemic by artificial ventilation with 8% oxygen in nitrogen (Fi0 2 = 0.08) (group I). Seven animals were ventilated with II % oxygen (Fi0 2 = 0.11 ) (group 2). Five animals were ventilated with 14% ox ygen (Fi0 2 = 0. 14) (group 3), and

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Stabilization of Unstable Components in Langmuir–Blodgett Film System by Introduction of Water-Soluble Antioxidants

Iriyama¹,

Yoshiura²

1989

Chemistry Letters

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Liquid chromatographic determination of uric acid and ascorbic acid in rat retinae after ophthalmic artery and optic nerve ligation

Cited by 3 publications

References 12 publications

Prooxidant–antioxidant balance, peroxide and catalase activity in the aqueous humour and serum of patients with exfoliation syndrome or exfoliative glaucoma

Prooxidant–antioxidant balance, peroxide and catalase activity in the aqueous humour and serum of patients with exfoliation syndrome or exfoliative glaucoma

Changes in Oxypurine Concentrations in Vitreous Humor of Pigs during Hypoxemia and Post-Mortem

Stabilization of Unstable Components in Langmuir–Blodgett Film System by Introduction of Water-Soluble Antioxidants

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