Liquid Chromatographic Approaches to Determination of B-Lactam Antibiotic Residues in Milk and Tissues

Moats, W. A.

doi:10.1007/978-1-4615-3356-6_11

Cited by 5 publications

(2 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6 2 µg std; detection UV-200 nm, 0.1 AUFS, 1 mL/min. recovery of antibiotic residues from tissues and milk (Moats, 1990). Extraction/deproteinization with 1:1 methanol gave fairly efficient precipitation of proteins when samples were buffered with 0.1 M (pH 4.6) NH4H2PO4.…”

Section: Resultsmentioning

confidence: 99%

Determination of lincomycin in milk and tissues by reversed-phase liquid chromatography

Moats¹

1991

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Lincomycin cannot be distinguished from other antibiotics by microbiological tests. A procedure was developed for determination of lincomycin residues in milk and tissues using high-performance liquid chromatography. For extraction/deproteinization, samples were mixed/blended with 3 volumes of 0.1NH4H2PO4. An equal volume of methanol was added, and the mixture was filtered. Then 1.5 volumes of acetonitrile was added, and the mixture was refiltered. The filtrate was concentrated to <4 mL by evaporation. An automated HPLC system was used for cleanup. An aliquot of sample extract (2 mL) was loaded onto a reversed-phase Cig column in the HPLC system with the mobile phase 0.01 NH4H2PO4-O.OO5 M Me4NCl. Lincomycin was eluted with an acetonitrile gradient. A narrow fraction containing the lincomycin was collected and rechromatographed by reversed-phase HPLC at pH 7.5. Mean recoveries were 89-99 % with detection limits of 20 (muscle, milk) and 50 ppb (liver,

show abstract

Section: Resultsmentioning

confidence: 99%

Determination of lincomycin in milk and tissues by reversed-phase liquid chromatography

Moats¹

1991

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…High performance liquid chromatography (HPLC) analysis of OTC offers a selective low level detection method and many HPLC methods have been reported for the determination of residual OTC in biological samples (Jurgens, 1981;Sporns et al, 1986;Oka et al, 1987;Renon et al, 1988;Moats, 1990 (1) Simple dilution (1:2.5) in distilled water involved adding 2 g honey samples spiked at 50 and 100 ppb (n = 2) to 5 mL water and diluted 1 to 10 with Buffer 1 (dilution factor = 25). In the test solution the concentration of OTC was 2 ng/mL (50 ppb) and 4 ng/mL (100 ppb).…”

Section: Introductionmentioning

confidence: 99%

Determination of residual oxytetracycline in honey with an immunoassay kit

1996

View full text Add to dashboard Cite

Summary — The application of the ELISA kit Ridascreen 'Tetracyclins" (from R-Biopharm GmbH, Darmstadt, Germany) for the semiquantitative analysis of oxytetracycline (OTC) residues in honey samples was investigated. To assess the extension of the kit to this use, some OTC-positive honey samples, spiked with OTC at concentrations ranging from 2 to 250 ppb, were prepared, purified and submitted to the ELISA test. For every set of positive honey samples, a set of OTC-standard solutions in water at the same concentration value of the positive samples and an OTC-negative (blank) control honey sample, were tested. Also, the efficiency of three different chemical purification procedures of honey samples was studied and verified: (1) the honey samples were diluted in water; (2) the honey samples were purified on a prepacked reversed-phase column (C-18 cartridges); (3) the honey samples were purified by a two-step system using a C-18 cartridge and a Diol-column. The mean recoveries of OTC were 90.4% (CV = 2.6%) for the test using C-18 (2) and 88.6% (CV = 2.1 %) for the test using both C-18 and Diol cartridges (3). The detection limits were lower than 50 ppb. honey / residue / oxytetracycline / immunoenzymatic assay

show abstract

Development, Optimization, and Validation of Methods for Quantification of Veterinary Drug Residues in Complex Food Matrices Using Liquid-Chromatography—A Review

et al. 2019

View full text Add to dashboard Cite

Liquid Chromatographic Approaches to Determination of B-Lactam Antibiotic Residues in Milk and Tissues

Cited by 5 publications

References 16 publications

Determination of lincomycin in milk and tissues by reversed-phase liquid chromatography

Determination of lincomycin in milk and tissues by reversed-phase liquid chromatography

Determination of residual oxytetracycline in honey with an immunoassay kit

Development, Optimization, and Validation of Methods for Quantification of Veterinary Drug Residues in Complex Food Matrices Using Liquid-Chromatography—A Review

Contact Info

Product

Resources

About