Determination of lincomycin in milk and tissues by reversed-phase liquid chromatography

Moats, W. A.

doi:10.1021/jf00010a024

Cited by 42 publications

(4 citation statements)

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“…Concentrations of LM and CM in the biological materials were determined by high-performance liquid chromatography (HPLC) with UV-detection described by Moats (1991) and Flaherty et al (1988), respectively, with a slight modi®cation. An octadecyl column [Lichrosphere RP ± 18, 5 lm, 250´4 mm intradermal (i.d.…”

Section: Drug Determinationmentioning

confidence: 99%

Effects of altered plasma α‐1‐acid glycoprotein levels on pharmacokinetics of some basic antibiotics in pigs: simulation analysis

Kuroha

Son

Shimoda

2001

Vet Pharm & Therapeutics

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Effects of altered plasma alpha-1-acid glycoprotein (AGP) levels on pharmacokinetic parameters of basic antimicrobials, erythromycin (EM), lincomycin (LM) and clindamycin (CM) were evaluated in pigs by simulation analysis. Intravenous (i.v.) injections of EM, LM and CM were performed to obtain pharmacokinetic parameters in healthy conditions. Binding parameters were obtained from an in vitro study using ultrafiltration. Simulation studies indicated that an increase of plasma AGP levels resulted in a decrease of both volume of distribution at steady state (Vdss) and total body clearance (Cltot) for all the drugs. Elimination rate constant for LM was almost unchanged by an increase of plasma AGP levels, whereas those for EM and CM were increased. Plasma concentration-time profiles at a high AGP level (often observed in pathophysiological conditions) were also simulated. All of the total plasma concentration-time profiles were different from those at normal AGP level. The differences were characterized by a higher initial concentration with faster or similar elimination. Unbound plasma concentration-time profile of LM was unaffected by AGP levels, whereas EM and CM were eliminated from plasma more rapidly at high AGP level. These results suggested that adjustment of dosage regimen of EM and CM is required in pathophysiological conditions, but that of LM is not required.

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Section: Drug Determinationmentioning

confidence: 99%

Effects of altered plasma α‐1‐acid glycoprotein levels on pharmacokinetics of some basic antibiotics in pigs: simulation analysis

Kuroha

Son

Shimoda

2001

Vet Pharm & Therapeutics

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“…Lincomycin. LM (Sigma, St. Louis, USA) concentrations were determined by a reverse-phase HPLC procedure (Moats, 1991) with slight modification. The HPLC system was at the same setting as that used for TMP determination, except for the analytical column (Shim-pack CLC-ODs, 5 pn, 150 x 6 mm ID, Shimadzu Corporation).…”

Section: Drug-protein Binding Studymentioning

confidence: 99%

Contribution of æ₁‐acid glycoprotein to plasma protein binding of some basic antimicrobials in pigs

Son

Hariya

Shimoda

et al. 1996

Vet Pharm & Therapeutics

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Protein binding kinetics of basic antimicrobials including trimethoprim (TMP), erythromycin (EM), lincomycin (LM) and clindamycin (CM) were studied using porcine plasma, albumin and alpha 1-acid glycoprotein (AGP). Rosenthal plots of these basic drugs in porcine plasma suggest saturable and non-saturable binding. Dissociation constants (kd) and binding capacity (Bmax) for saturable binding were as follows: TMP, kd = 8.58 mumol/L, Bmax = 5.26 mumol/L; EM, kd = 2.72 mumol/L, Bmax = 3.06 mumol/L, LM, kd = 3.96 mumol/L, Bmax = 6.58 mumol/L and CM, kd = 4.43 mumol/L, Bmax = 21.7 mumol/L. The proportionality constants (Bmax2/kd2) for non-saturable binding were 0.29 in TMP, 0.52 in EM, 0.17 in LM and 3.2 in CM. The kds of the drugs in porcine AGP solution were determined by a fluorescence quenching method, using 1-anilino-8-naphthalene sulphonate (ANS) as a fluorescent probe: 9.51 mumol/L in TMP, 1.89 mumol/L in EM, 4.48 mumol/L in LM and 9.69 mumol/L, in CM. Comparable kd values between porcine plasma and AGP solution indicated that AGP is a major saturable binder in porcine plasma. Binding property to porcine albumin presented linearity, showing the following proportionality constants: 0.23 in TMP, 0.38 in EM, 0.01 in LM and 0.76 in CM. The comparable proportionality constants of TMP and EM between porcine plasma and albumin solution indicate that albumin is a major non-saturable binder, whereas proportionality constants of LM and CM in albumin solution compared to those in porcine plasma were low, implying another non-saturable binder, i.e. lipoprotein. Simulation curve of drug-binding percentage vs. AGP concentrations showed that in pigs under a pathologic state, or during early growth stage with high AGP levels, AGP could be a main contributor to drug-plasma protein binding for all drugs examined. The increase of AGP from normal to pathological concentrations induced a decrease in the unbound fraction: LM > CM > EM > TMP in order of AGP contribution to drug binding. Therefore, the disposition and efficacy of basic antimicrobials which bind to AGP with high affinity could be markedly influenced by altered AGP levels, implying AGP contribution to pharmacokinetics and pharmacodynamics.

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“…Numerous available methods for the determination LIN in different matrices are generally based on chromatographic techniques with various detection systems − . Immunoassay as a screening test has an advantage over chromatographic procedures in simplicity, sensitivity, and capability of high sample throughput.…”

Section: Introductionmentioning

confidence: 99%

Development of a Competitive Indirect ELISA for the Determination of Lincomycin in Milk, Eggs, and Honey

Burkin

Galvidis

2010

J. Agric. Food Chem.

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Polyclonal antibodies to lincomycin (LIN) were developed in rabbit as a result of immunization with BSA-LIN conjugate. Periodate oxidizing of hapten was the common step of both immunogen synthesis and preparation of conjugated antigens for coating plates (homologous and heterologous). Several ELISA variants on a base of the different antigens immobilized on polystyrene were compared. Heterology of solid-phase antigens was provided with relative hapten clindamycin (CLIN) and ethylene- or hexanediamine as spacer arm between hapten and carrier. The spacer insertion yielded no desirable effect, whereas gelatin-CLIN assay variant showed better test characteristics in comparison with the homologous one, although insignificant (IC(50) was 9.15 vs 18.3 ng mL(-1)). The detection limits of the developed test, being estimated as 0.43 ng mL(-1) (milk) and 0.65 ng mL(-1) (eggs), were sufficient to measure maximum residue levels for LIN in examined matrices. This value for honey was 1.9 ng mL(-1) (1.3 μg kg(-1)). The assay sensitivity was enough to dilute milk, egg, and honey samples by 10-100 times to minimize matrix effect. The examination of matrix effect and simple ways of its overcoming are detailed in the paper. The developed assay showed 111% cross-reactivity with CLIN; therefore, it is suitable for the determination of both lincosamides.

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Determination of lincomycin in milk and tissues by reversed-phase liquid chromatography

Cited by 42 publications

References 4 publications

Effects of altered plasma α‐1‐acid glycoprotein levels on pharmacokinetics of some basic antibiotics in pigs: simulation analysis

Effects of altered plasma α‐1‐acid glycoprotein levels on pharmacokinetics of some basic antibiotics in pigs: simulation analysis

Contribution of æ₁‐acid glycoprotein to plasma protein binding of some basic antimicrobials in pigs

Development of a Competitive Indirect ELISA for the Determination of Lincomycin in Milk, Eggs, and Honey

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Determination of lincomycin in milk and tissues by reversed-phase liquid chromatography

Cited by 42 publications

References 4 publications

Effects of altered plasma α‐1‐acid glycoprotein levels on pharmacokinetics of some basic antibiotics in pigs: simulation analysis

Effects of altered plasma α‐1‐acid glycoprotein levels on pharmacokinetics of some basic antibiotics in pigs: simulation analysis

Contribution of æ1‐acid glycoprotein to plasma protein binding of some basic antimicrobials in pigs

Development of a Competitive Indirect ELISA for the Determination of Lincomycin in Milk, Eggs, and Honey

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Contribution of æ₁‐acid glycoprotein to plasma protein binding of some basic antimicrobials in pigs