Liquid chromatographic analysis of halofantrine from dosage form and its metal interaction studies

Abstract

The reversed-phase high-performance liquid chromatographic method has been developed for the determination of halofantrine from dosage form in presence of losartan potassium as an internal standard. Separation was performed on a Chrompack hypersil C 18 (150 9 4.6 mm) analytical reversed-phase column. Mobile phase consisted of methanol-water (70:30, v/v) pH was adjusted to 3.2 with 85% orthophosphoric acid. Mobile phase was pumped at a flow rate of 0.5 mL min -1 and UV detection was performed at 248 nm. The method was validated for linearity, precision, accuracy and specificity. The linearity was observed in the concentration range 1.0-50 lg mL -1 with correlation coefficient (r 2 ) of 0.9998. Accuracy was ranged 98-100% with precision less than 1%. The limits of detection (LOD) and (LOQ) were 0.01 and 0.03 lg mL -1 , respectively. This method was applied to study the drug-metal (calcium, magnesium, manganese, cobalt, ferrous, ferric chromium, zinc, nickel, cadmium, iron and copper) interaction studies which were carried out at 37°C. These studies were beneficial to determine the drug in therapeutic concentrations inside human body as well as its complexation with metal ions. The proposed method is rapid, accurate, economical and selective because of its sensitivity and reproducibility.

Development of Liquid Chromatography? UV Method for Simultaneous Determination of Leflunomide and NSAIDs in API and Pharmaceutical Formulations: It?s Application to In vitro Interaction Studies

“…A number of methods for the simultaneous determination of coadminstered drugs have also been reported by our research group as simultaneous determination of rosuvastatin, lisinopril, captopril and enalapril [19], lisinopril, [20] verapamil [21], captopril [22], ceftriaxone sodium [23], diltiazem [24] rosuvastatin [25] and sparfloxacin [26] with NSAIDs in API, pharmaceutical formulations and human serum by RP-HPLC. Arayne et al [27][28][29][30][31][32][33][34] and Sultana et al [35][36][37][38][39][40] have previously reported, a number of methods for drug-drug interaction studies using UV-visible spectroscopic and RP-HPLC. In continuation of this work we have attempted to study in vitro interactions of leflunomide with flurbiprofen, ibuprofen, diclofenac sodium and mefenamic acid (Figure 1), the experiment was accomplished by the use of UV-visible spectroscopic and RP-HPLC techniques.…”