Lipoxygenases and plant cell metabolism regulation

Pokotylo, Igor; Ys, Kolesnikov; Mv, Derevyanchuk; Ai, Kharitonenko; Kravets, V. S.

doi:10.15407/ubj87.02.041

Cited by 7 publications

(7 citation statements)

References 93 publications

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“…During pathogen attack, plants start releasing metabolites with preventive and toxic effects, whereas other metabolites function as signaling molecules to stimulate defense-related responses. For biting and chewing types of herbivores, the LOX pathway plays a significant role in defense mechanisms by foremost oxylipins such as 10-OPDA (a positional isomer of 12-OPDA), 12-OPDA via the action of 13-LOXs, and 10-oxo-11-phytoenoic acid (10-OPEA) via the action of 9-LOXs [ 100 ]. Herbivory results in an anti-herbivore oxidative shift, which results in either direct or indirect oxidative damage to herbivores and transmits the signals for managing the defense mechanism of plants [ 101 ].…”

Section: Lox In Pathogen Attackmentioning

confidence: 99%

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Specific Roles of Lipoxygenases in Development and Responses to Stress in Plants

Singh

Arif

Miszczuk

et al. 2022

Plants

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Lipoxygenases (LOXs), naturally occurring enzymes, are widely distributed in plants and animals. LOXs can be non-sulfur iron, non-heme iron, or manganese-containing dioxygenase redox enzymes. LOXs catalyze the oxidation of polyunsaturated fatty acids into fatty acid hydroperoxides. Linolenic acid, a precursor in the jasmonic acid (JA) biosynthesis, is converted to 12-oxo-phytodienoic acid through oxygenation with LOX, allene oxide synthase, and allene oxide cyclase. Moreover, JA participates in seed germination, fruit ripening, senescence, and many other physio-biochemical processes. LOXs also play crucial roles in defense responses against biotic stress, i.e., insects, pests, pathogenic attacks, and abiotic stress, such as wounding, UV-rays, extreme temperature, oxidative stress, and drought.

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Section: Lox In Pathogen Attackmentioning

confidence: 99%

“…The activity of LOX is considered a good biological marker in the field of plant physiology [ 95 , 100 ]. Plants facing thermal stress, pathogen infection, and mechanical damage increase the activity of 13-LOX [ 47 , 48 , 49 ].…”

Section: Lox Activity As a Molecular Marker For Stress Tolerance/stre...mentioning

confidence: 99%

Specific Roles of Lipoxygenases in Development and Responses to Stress in Plants

Singh

Arif

Miszczuk

et al. 2022

Plants

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“…LOX activity is regarded as a biological marker of the plant physiological state [4,6,103]. It was shown that following the effects of high temperatures, mechanical dama ges, pathogen infection the 13-LOX activity increa ses [26,77,81].…”

Section: Lox Activity Characteristics As Stress Tolerance Molecular Mmentioning

confidence: 99%

Lipoxygenases and their metabolites in formation of plant stress tolerance

Бабенко¹,

Shcherbatiuk²,

Skaterna³

et al. 2017

Ukr.Biochem.J.

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“…In addition to the increase in the de novo synthesis of the TdLOX2 isoform due to the induction of TdLpx-A2 gene expression, a regulation of the enzyme at post-transcriptional and/or post-translational level cannot be excluded. At this regard, it is known that in plants exposed to stress conditions LOXs can be regulated by other mechanisms besides gene induction, which include the alternative splicing, phosphorylation, allosteric modulation and protein-protein interaction [ 52 ]. Anyway, our findings represent a relevant progress on this topic because they represent the first detailed evidence of the significance of LOXs in general, and of the TdLOX2 in particular, in the physiological processes and the stress-related responses of durum wheat.…”

Section: Resultsmentioning

confidence: 99%

The Molecular and Functional Characterization of the Durum Wheat Lipoxygenase TdLOX2 Suggests Its Role in Hyperosmotic Stress Response

Menga

Trono

2020

Plants

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In plants, lipoxygenases (LOXs) are involved in various processes, such as growth, development, and response to stress cues. In the present study, the expression pattern of six durum wheat LOX-encoding genes (TdLpx-B1.1, TdLpx-B1.2, TdLpx-A2, TdLpx-B2, TdLpx-A3 and TdLpx-B3) under hyperosmotic stress was investigated. With osmotic (0.42 M mannitol) and salt (0.21 M NaCl) stress imposed at the early stages of seedling growth, a strong induction of the TdLpx-A2 gene expression in the shoots paralleled an equally strong increase in the LOX activity. Enhanced levels of malondialdehyde (MDA) and increased rates of superoxide anion generation were also observed as a result of the stress imposition. Sequence analysis of the TdLOX2 encoded by the TdLpx-A2 gene revealed that it belonged to the type-1 9-LOX group. When overexpressed in E. coli, TdLOX2 exhibited normal enzyme activity, high sensitivity to specific LOX inhibitors, with 76% and 99% inhibition by salicylhydroxamic and propyl gallate, respectively, and a preference for linoleic acid as substrate, which was converted exclusively to its corresponding 13-hydroperoxide. This unexpected positional specificity could be related to the unusual TV/K motif that in TdLOX2 replaces the canonical TV/R motif of 9-LOXs. Treatment of seedlings with propyl gallate strongly suppressed the increase in LOX activity induced by the hyperosmotic stress; the MDA accumulation was also reduced but less markedly, whereas the rate of superoxide anion generation was even more increased. Overall, our findings suggest that the up-regulation of the TdLpx-A2 gene is a component of the durum wheat response to hyperosmotic stress and that TdLOX2 may act by counteracting the excessive generation of harmful reactive oxygen species responsible for the oxidative damages that occur in plants under stress.

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Lipoxygenases and plant cell metabolism regulation

Cited by 7 publications

References 93 publications

Specific Roles of Lipoxygenases in Development and Responses to Stress in Plants

Specific Roles of Lipoxygenases in Development and Responses to Stress in Plants

Lipoxygenases and their metabolites in formation of plant stress tolerance

The Molecular and Functional Characterization of the Durum Wheat Lipoxygenase TdLOX2 Suggests Its Role in Hyperosmotic Stress Response

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