Specific Roles of Lipoxygenases in Development and Responses to Stress in Plants

Singh, Priyanka; Arif, Yamshi; Miszczuk, Edyta; Bajguz, Andrzej; Hayat, Shamsul

doi:10.3390/plants11070979

Cited by 79 publications

(49 citation statements)

References 133 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LOX exerts multiple functions in higher plants and is considered a biological marker of stress because it mediates the JA biosynthesis [ 5 , 7 , 27 , 28 , 29 ]. The number of LOX isozymes and genes changes depending on the plant species, analyzed organs, and environmental conditions [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…LOXs can be categorized as 9- and 13-lipoxygenases, according to the carbon position (either C9 or C13) where the oxygen is inserted in both C18:2 and C18:3. These enzymes play significant functions in plant development and stress response [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ]. Fatty acids are indispensable components in plant cells [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lipoxygenase (LOX) in Sweet and Hot Pepper (Capsicum annuum L.) Fruits during Ripening and under an Enriched Nitric Oxide (NO) Gas Atmosphere

González‐Gordo

Cañas

Muñoz‐Vargas

et al. 2022

IJMS

View full text Add to dashboard Cite

Lipoxygenases (LOXs) catalyze the insertion of molecular oxygen into polyunsaturated fatty acids (PUFA) such as linoleic and linolenic acids, being the first step in the biosynthesis of a large group of biologically active fatty acid (FA)-derived metabolites collectively named oxylipins. LOXs are involved in multiple functions such as the biosynthesis of jasmonic acid (JA) and volatile molecules related to the aroma and flavor production of plant tissues, among others. Using sweet pepper (Capsicum annuum L.) plants as a model, LOX activity was assayed by non-denaturing polyacrylamide gel electrophoresis (PAGE) and specific in-gel activity staining. Thus, we identified a total of seven LOX isozymes (I to VII) distributed among the main plant organs (roots, stems, leaves, and fruits). Furthermore, we studied the FA profile and the LOX isozyme pattern in pepper fruits including a sweet variety (Melchor) and three autochthonous Spanish varieties that have different pungency levels (Piquillo, Padrón, and Alegría riojana). It was observed that the number of LOX isozymes increased as the capsaicin content increased in the fruits. On the other hand, a total of eight CaLOX genes were identified in sweet pepper fruits, and their expression was differentially regulated during ripening and by the treatment with nitric oxide (NO) gas. Finally, a deeper analysis of the LOX IV isoenzyme activity in the presence of nitrosocysteine (CysNO, a NO donor) suggests a regulatory mechanism via S-nitrosation. In summary, our data indicate that the different LOX isozymes are differentially regulated by the capsaicin content, fruit ripening, and NO.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lipoxygenase (LOX) in Sweet and Hot Pepper (Capsicum annuum L.) Fruits during Ripening and under an Enriched Nitric Oxide (NO) Gas Atmosphere

González‐Gordo

Cañas

Muñoz‐Vargas

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…The indirect mechanisms include the induction of acquired systematic resistance, plant pathogen molecular patterns (PAMPs) which in turn trigger the immunity and plant resistance proteins (Yu et al, 2022 ). Microorganisms solubilize the phosphorus and zinc, fixing the nitrogen and other macro- and micronutrients which promote the growth of the plants under biotic stress condition by providing nutrition (Singh et al, 2022a ). They also enhance the stress resistance in plants by expressing the gene of phytohormones and stress-related metabolite.…”

Section: Introductionmentioning

confidence: 99%

Overview of biofertilizers in crop production and stress management for sustainable agriculture

Chaudhary

Singh²,

Chaudhary³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

With the increase in world population, the demography of humans is estimated to be exceeded and it has become a major challenge to provide an adequate amount of food, feed, and agricultural products majorly in developing countries. The use of chemical fertilizers causes the plant to grow efficiently and rapidly to meet the food demand. The drawbacks of using a higher quantity of chemical or synthetic fertilizers are environmental pollution, persistent changes in the soil ecology, physiochemical composition, decreasing agricultural productivity and cause several health hazards. Climatic factors are responsible for enhancing abiotic stress on crops, resulting in reduced agricultural productivity. There are various types of abiotic and biotic stress factors like soil salinity, drought, wind, improper temperature, heavy metals, waterlogging, and different weeds and phytopathogens like bacteria, viruses, fungi, and nematodes which attack plants, reducing crop productivity and quality. There is a shift toward the use of biofertilizers due to all these facts, which provide nutrition through natural processes like zinc, potassium and phosphorus solubilization, nitrogen fixation, production of hormones, siderophore, various hydrolytic enzymes and protect the plant from different plant pathogens and stress conditions. They provide the nutrition in adequate amount that is sufficient for healthy crop development to fulfill the demand of the increasing population worldwide, eco-friendly and economically convenient. This review will focus on biofertilizers and their mechanisms of action, role in crop productivity and in biotic/abiotic stress tolerance.

show abstract

“…They play important roles in plant development and tolerance to abiotic and biotic stresses (Chen et al, 2022;Mou et al, 2022). LOXs potentially participate in signaling caused by stressor effects and are considered as a stress biomarkers against biotic and abiotic stresses (Singh et al, 2022). Lim et al (2015) reported that CaLOX-overexpressing Arabidopsis plants exhibited tolerance phenotypes to drought and high salt stresses via rapid scavenging of ROS and by inducing high expression of ABA (abscisic acid) and stress-responsive marker genes.…”

Section: Introductionmentioning

confidence: 99%

“…They play important roles in plant development and tolerance to abiotic and biotic stresses ( Chen et al., 2022 ; Mou et al., 2022 ). LOXs potentially participate in signaling caused by stressor effects and are considered as a stress biomarkers against biotic and abiotic stresses ( Singh et al., 2022 ). Lim et al.…”

Section: Introductionmentioning

confidence: 99%

Melatonin induces drought tolerance by modulating lipoxygenase expression, redox homeostasis and photosynthetic efficiency in Arachis hypogaea L

Shreya

Supriya²,

Gudipalli³

2022

Front. Plant Sci.

View full text Add to dashboard Cite

Melatonin (N-acetyl-5-hydroxy tryptamine), a multipotent biomolecule is well known for its ability to confer tolerance to several abiotic and biotic stresses. The regulation of melatonin-mediated drought tolerance in drought-distinguished varieties can be different due to discriminating redox levels. The present study was focused on assessing the effects of melatonin priming against polyethylene glycol (PEG)-induced stress with respect to the antioxidant system, photosynthetic parameters, lipoxygenase expression, JA and ABA levels in drought-sensitive (Kadiri-7) and drought-tolerant (Kadiri-9) varieties. Exogenous melatonin alleviated the drought stress effects in sensitive variety (Kadiri-7) by increasing the endogenous melatonin content with an improved antioxidant system and photosynthetic attributes. The primed stressed plants of the sensitive variety exhibited reduced expression and activity of the chlorophyll degrading enzymes (Chl-deg PRX, pheophytinase and chlorophyllase) with a concomitant increase in chlorophyll content in comparison to unprimed controls. Interestingly, melatonin priming stimulated higher expression and activity of lipoxygenase (LOX) as well as enhanced the expression of genes involved in the synthesis of jasmonic acid (JA) including its content in drought stressed plants of the sensitive variety. The expression of NCED3 (involved in ABA-biosynthesis) was upregulated while CYP707A2 (ABA-degradation) was downregulated which corresponded with higher ABA levels. Contrastingly, priming caused a decrease in endogenous melatonin content under drought stress in tolerant variety (Kadiri-9) which might be due to feedback inhibition of its synthesis to maintain intracellular redox balance and regulate better plant metabolism. Furthermore, the higher endogenous melatonin content along with improved antioxidant system, photosynthetic efficiency and LOX expression associated with the increased levels of JA and ABA in unprimed stressed plants of the tolerant variety (Kadiri-9) is pointing towards the effectiveness of melatonin in mediating drought stress tolerance. Overall, exogenous melatonin alleviated the adverse effects of drought stress in sensitive variety while having no add-on effect on drought stress responses of tolerant variety which is inherently equipped to withstand the given duration of drought stress treatment.

show abstract

Specific Roles of Lipoxygenases in Development and Responses to Stress in Plants

Cited by 79 publications

References 133 publications

Lipoxygenase (LOX) in Sweet and Hot Pepper (Capsicum annuum L.) Fruits during Ripening and under an Enriched Nitric Oxide (NO) Gas Atmosphere

Lipoxygenase (LOX) in Sweet and Hot Pepper (Capsicum annuum L.) Fruits during Ripening and under an Enriched Nitric Oxide (NO) Gas Atmosphere

Overview of biofertilizers in crop production and stress management for sustainable agriculture

Melatonin induces drought tolerance by modulating lipoxygenase expression, redox homeostasis and photosynthetic efficiency in Arachis hypogaea L

Contact Info

Product

Resources

About