Lipoprotein lipase activity at onset of development of white adipose tissue in newborn rats

Pequignot-Planche, E.; Gasquet, P. de; Boulange, A. Quignard; Tonnu, N. T.

doi:10.1042/bj1620461

Cited by 31 publications

(16 citation statements)

References 18 publications

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“…So we suggest that the occurrence of LPL allows the neonatal liver to directly take up circulating triacylglyce rols present in chylomicra, which are abun dant during lactation because of the milk ingestion, and that could be of special impor tance for the neonates. It has been estimated that adipose tissue contains more than 54% of the total LPL activity in fed adult rats [27], but neonatal rats have a very small amount of adipose tissue and also the LPL activity in this tissue is very low at birth [7,25]. So neonates lack a major site for lipoprotein-triacylglycerol removal.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Lipoprotein Lipase Activity in the Newborn Rat Liver

Reina¹,

Vilaró²,

Ramı́rez³

et al. 1987

Neonatology

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Acetone ether powders of livers from starved newborn rats were applied to heparin-Sepharose affinity columns. The so-called hepatic triacylglycerol lipase was eluted with 0.9 M NaCl and a second lipolytic activity peak was eluted with 1.5 M NaCl. The behaviour of this 1.5 M NaCl-eluted fraction against increasing concentrations of serum, NaCl, protamine sulfate and heparin in the assay mixture was almost identical to that shown by partially purified lipoprotein lipase from adult rat adipose tissue, and clearly different from that shown by partially purified hepatic triacylglycerol lipase from the adult rat liver. We conclude that the newborn rat liver contains a lipoprotein lipase activity with similar properties to those found in adult adipose tissue lipoprotein lipase activity. It is suggested that this enzyme enables the neonatal liver to take up circulating triacylglycerols directly.

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Section: Discussionmentioning

confidence: 99%

Characterization of Lipoprotein Lipase Activity in the Newborn Rat Liver

Reina¹,

Vilaró²,

Ramı́rez³

et al. 1987

Neonatology

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“…Serum concentrations of total carnitine, however, were not different in the infants who were small for gestational age (mean (SEM) 26 Open symbols refer to the infants ofappropriate weight and solid symbols to the infants who were smallfor gestational age. Squares refer to the infants weighing more than 1500 g and circles to the infants of very low birth weight weighing less than 1500 g. …”

Section: Serum Concentration and Urinary Excretion Of Carnitinementioning

confidence: 98%

“…The activities of lipoprotein and hepatic lipase in plasma taken 15 minutes after a heparin bolus of 100 IU/kg had been given and the concentrations of carnitine in serum and urine were measured. The mean gestational age was 31 weeks (range [26][27][28][29][30][31][32][33][34][35] weeks) and birth weight 1580 g (range 840-2280 g). Thirteen infants weighed under 1500 g at birth (very low birth weight), 20 were of appropriate weight for gestational age and six were small for gestational age.…”

mentioning

confidence: 99%

Lipoprotein lipase, hepatic lipase, and carnitine in premature infants.

Rovamo

Nikkilä

Raivio

1988

Archives of Disease in Childhood

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SUMMARY Twenty six preterm infants were studied at the age of 2, 7, and 26 days. The activities of lipoprotein and hepatic lipase in plasma taken 15 minutes after a heparin bolus of 100 IU/kg had been given and the concentrations of carnitine in serum and urine were measured. The mean gestational age was 31 weeks (range 26-35 weeks) and birth weight 1580 g (range 840-2280 g). Thirteen infants weighed under 1500 g at birth (very low birth weight), 20 were of appropriate weight for gestational age and six were small for gestational age. Lipoprotein lipase activity was higher in the preterm infants of appropriate weight than in the infants of very low birth weight and those who were small for gestational age. At the age of 2 or 7 days the activity of lipoprotein lipase in the preterm infants (mean (SEM) 46-2 (4.3) ,tmol free fatty acid/mV/hour) was, however, higher than in term infants and adults. Multivariate regression analyses showed that weight and relative birth weight together explained 58% of the variance of lipoprotein lipase activity but only 3% of the variance of hepatic lipase activity. Serum carnitine concentration was lower in the preterm infants than in term infants. Urinary excretion of carnitine increased progressively with age but was independent of serum concentration and carnitine intake. Urinary excretion of total carnitine was significantly greater in the infants who were small for gestational age (mean (SEM) 754 (203) nmol/mg of creatinine, n=6) than in the infants of appropriate weight (161 (22-0) nmol/mg of creatinine, n= 12) but acyl/free carnitine ratio was smaller in the infants who were small for gestational age than in infants of appropriate weight (0-56 v 5-5). The

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“…These workers suggested a regulatory role for lipoprotein lipase in the control of adipocyte lipid filling during early development [Hietanen and Greenwood, 1977]. Other biochemical studies extended these findings [Hietanen and Greenwood, 1977] to other depots in the young rat [Cryer and Jones, 1979;Pequignot-Planche et al, 1977], These biochemical studies have not estab lished the temporal and spatial relationships between LPL development and capillary development.…”

Section: Introductionmentioning

confidence: 75%

Histochemically Detectable Lipoprotein Lipase Activity in Adipose Tissue of Pigs and Normal and Decapitated Pig Fetuses

Hausman¹

1982

Cells Tissues Organs

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The development of adipose tissue lipoprotein lipase (LPL) activity was determined cytochemically in sections from normal and decapitated pig fetuses. Cellular LPL activity was first evident in 60-day-old fetuses. Capillaries were stained for LPL activity in 85-day-old and older fetuses and in young pigs. Cellular synthesis of LPL and capillary staining were not concurrent events. LPL activity developed after adipocyte differentiation and after structural development of capillaries. The cellular and capillary staining of fat cell clusters throughout development and in decapitated fetuses indicated a possible inverse relationship between cellular synthesis of LPL and capability of capillaries to transport LPL to luminal sides. These observations indicate that LPL activity develops in a pattern quite distinct from that observed in vitro. This discrepancy may be due to the involvement of blood vessels in the in vivo developmental pattern.

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Lipoprotein lipase activity at onset of development of white adipose tissue in newborn rats

Cited by 31 publications

References 18 publications

Characterization of Lipoprotein Lipase Activity in the Newborn Rat Liver

Characterization of Lipoprotein Lipase Activity in the Newborn Rat Liver

Lipoprotein lipase, hepatic lipase, and carnitine in premature infants.

Histochemically Detectable Lipoprotein Lipase Activity in Adipose Tissue of Pigs and Normal and Decapitated Pig Fetuses

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