Histochemically Detectable Lipoprotein Lipase Activity in Adipose Tissue of Pigs and Normal and Decapitated Pig Fetuses

Hausman, Gary

doi:10.1159/000145598

Cited by 9 publications

References 8 publications

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Structural and histochemical aspects of perirenal adipose tissue in fetal pigs: Relationships between stromal‐vascular characteristics and fat cell concentration and enzyme activity

Hausman¹,

Thomas²

1986

Journal of Morphology

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Samples of perirenal adipose tissue were obtained from four fetuses from each of seven crossbred gilts at each of three stages of gestation: 70, 90, and 110 days. Samples were routinely prepared for histochemistry and histology. At each age, the largest fat cell clusters were consistently located near points where large blood vessels entered the loose connective tissue. Cell-cluster size decreased with distance from the entry points of large blood vessels. Fat cells proximal to entry points of large arterioles and fat cells distal to entry points of large arterioles were the same size. Enzyme cytochemistry disclosed that reactions for glucose-6-phosphate dehydrogenase (G6PDH), lipoprotein lipase (LPL) and NADH-TR enzymes were reduced in distal (relative to entry points of large arterioles) adipocytes compared with proximal adipocytes. Reactions for succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH) in adipocytes were not influenced by location within the tissue. Small fat cell clusters with sparse capillary beds surround arterioles in distal areas of sections from fetuses at 70, 90, and 110 days of gestation. In the proximal areas of sections from 110-day-old fetuses, arterioles were surrounded by large fat cell clusters with dense capillary beds. These characteristics serve to distinguish perirenal depots from subcutaneous depots in the fetus.

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Structural and histochemical aspects of perirenal adipose tissue in fetal pigs: Relationships between stromal‐vascular characteristics and fat cell concentration and enzyme activity

Hausman¹,

Thomas²

1986

Journal of Morphology

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Neonatal Lipid Metabolism

Hamosh¹

1991

Principles of Perinatal-Neonatal Metabolism

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Nucleoside phosphatase and nucleotide tetrazolium reductase as markers of arteriolar differentiation in fetal pig tissue

Hausman

1985

Histochemistry

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Nucleoside phosphatase and nucleotide tetrazolium reductase reactions were studied as potential markers of arteriolar differentiation. Arteriolar systems were analyzed cytochemically and morphologically in a variety of fetal pig tissues at 70 and 110 days of gestation. In skeletal muscle and subcutaneous adipose tissue there were age dependent changes in phosphatase and reductase reactivity in arteriolar vessels. These changes were temporally associated with the morphological differentiation of the tunica medial arteriolar layer. There were no age dependent changes in the cytochemistry of arterioles in liver and cardiac muscle. In the youngest fetuses, arterioles in liver and cardiac muscle displayed a typical tunica medial layer (normal morphology). The cytochemical reactions (phosphatase, reductase) of arterioles in cardiac muscle and liver from 70 (and 110) days old fetuses were identical to cytochemical reactions of arterioles in muscle and adipose tissue from 110 days old fetuses. In the skin, there were age dependent increases in phosphatase reactive arterioles and capillaries. Capillary staining (phosphatase) and capillary bed size were inversely correlated in the skin. Capillaries in skeletal muscle, cardiac muscle, adipose tissue and liver were not phosphatase reactive. These results indicate that the morphological and cytochemical differentiation of arterioles is dependent on tissue and age in the fetal pig. Furthermore, several histochemical techniques (phosphatase, reductases) are validated as simple means to analyze arteriolar differentiation in general.

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Histochemically Detectable Lipoprotein Lipase Activity in Adipose Tissue of Pigs and Normal and Decapitated Pig Fetuses

Cited by 9 publications

References 8 publications

Structural and histochemical aspects of perirenal adipose tissue in fetal pigs: Relationships between stromal‐vascular characteristics and fat cell concentration and enzyme activity

Structural and histochemical aspects of perirenal adipose tissue in fetal pigs: Relationships between stromal‐vascular characteristics and fat cell concentration and enzyme activity

Neonatal Lipid Metabolism

Nucleoside phosphatase and nucleotide tetrazolium reductase as markers of arteriolar differentiation in fetal pig tissue

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