Lipopolysaccharide Induces Upregulation of Neutral Endopeptidase 24.11 on Human Neutrophils: Involvement of the CD14 Receptor

Fagny, Christine; Marchant, Arnaud; Prez, Eric De; Goldman, Michel; Deschodt-Lanckman, M

doi:10.1042/cs0890083

Cited by 6 publications

(8 citation statements)

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“…Our data confirm results from Koch, who as early as 1991 described CD13 as a monocytic activation‐related antigen, which is up‐regulated by LPS [7]. Neprilysin/CD10 expression on neutrophils has also been described as increasing within 1 h after LPS exposure [29]. IL‐10 treatment of PBMC resulted in a down‐regulation of both HLA‐DR and CD13 expression on monocytes.…”

Section: Discussionsupporting

confidence: 88%

Changes in monocytic expression of aminopeptidase N/CD13 after major trauma

Huschak

Nieden

Stuttmann

et al. 2003

Clinical and Experimental Immunology

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SUMMARYHLA-DR expression on monocytes as marker for monocytic function is severely depressed after major trauma. The membrane enzyme aminopeptidase N/CD13 can trigger help in antigen processing by MHC class II molecules of antigen-presenting cells. We determined the simultaneous expression of HLA-DR and CD13 on peripheral blood monocytes of patients with major trauma (injury severity score of ≥ 16). 1 : 1 conjugates of phycoerythrin (PE)-to-monoclonal antibody were used in combination with QuantiBRITE TM PE beads for a standardized quantification in terms of antibodies bound per cell (ABC). The very low expression of HLA-DR antigen on monocytes of patients at day 1 after major trauma confirmed previous results in the literature. Monocytic HLA-DR expression increased slowly to reach values in the lower range of healthy volunteers at day 14. Monocytic CD13 expression at day 1 showed values in the range of healthy volunteers, and a strong rise afterwards. Fourteen days after trauma, the monocytic expression of CD13 was still much higher than in the control group. Because lipopolysaccharide (LPS) and the anti-inflammatory cytokine interleukin (IL)-10 have been shown to be involved in the depressed HLA-DR expression on monocytes in trauma patients, we studied the in vitro effects of LPS and interleukin (IL)-10 on the expression of CD13 on monocytes prepared from the peripheral blood of healthy volunteers. Whereas a 3-day IL-10 treatment resulted in a down-regulation of both HLA-DR and CD13 expression on monocytes, LPS caused a down-regulation of HLA-DR but a rapid up-regulation of CD13 levels. Therefore we suggest that, with respect to monocytic CD13 expression, LPS rather than IL-10 could well be the explanation for monocytic surface molecules after severe injury, although other mediators with a CD13 regulating function have to be considered.

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Section: Discussionsupporting

confidence: 88%

Changes in monocytic expression of aminopeptidase N/CD13 after major trauma

Huschak

Nieden

Stuttmann

et al. 2003

Clinical and Experimental Immunology

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“…CD10 expression on the neutrophil surface seems to be up-regulated by many factors, such as lipopolysaccharide and granulocyte/macrophage-colony-stimulating factor (Connelly et al 1993;Fagny et al 1995). A close association of CD10 expression with some diseases has been described.…”

Section: Discussionmentioning

confidence: 95%

The ratio of neutrophils to lymphocytes and the phenotypes of neutrophils in patients with early gastric cancer

Hirashima¹,

Higuchi

Saito

et al. 1998

Journal of Cancer Research and Clinical Oncology

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We examined 55 patients (40 male, 15 female) who were diagnosed from 1987 to 1991 as having early gastric cancer (EGC) stage I according to the general rules of classification of the Japanese Research Society for Gastric Cancer. Of the 55 patients, 42 (30 male, 12 female) were alive in April 1992. The prognosis correlated well with the ratio of neutrophils to lymphocytes (N/L ratio) but not with the total number of white blood cells in the peripheral blood. The patients were divided into two groups according to their N/L ratio. Of the 29 patients with an N/L ratio less than 2, 27 were alive in 1992, whereas only 15 of the 26 patients with an N/L ratio of 2 or more were alive (chi2 analysis, P=0.0022). We further examined the phenotypes of neutrophils from 29 other patients with EGC at the time of diagnosis before surgical operation. These patients were divided into two groups: 17 patients with a low N/L ratio (less than 2) and 12 patients with a high N/L ratio (2 or more). CD10 and CD35 expressions on neutrophils from the patients with a low N/L ratio were lower than those from the patients with a high N/L ratio. The N/L ratio correlated well with both CD10 and CD35 expression, whereas no correlation was observed between the numbers of neutrophils and the expression of these phenotypes. The respiratory burst of neutrophils from the patients with a high N/L ratio was higher than that of neutrophils from the patients with a low N/L ratio, though there was no correlation in the phagocytic activity between both groups. It was thus suggested that the heterogeneity of neutrophils is, at least partly, related to the prognosis of patients with EGC.

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“…In fact, only 1 study has reported that CD10 expression may slightly increase in neutrophils incubated with lipopolysaccharide (LPS), but this was observed to occur in vitro. 45 Similarly, CD10 enzymatic activities have been shown to either decrease or increase upon in vitro stimulation of neutrophils with, respectively, phorbol 12-myristate 13-acetate, 46 or formyl-methionyl-leucyl phenylalanine, C5a, LPS, or granulocyte-macrophage colony-stimulating factor. 47 23 The latter cells, however, displayed their immunosuppressive properties via CD11b-mediated cell contacts and reactive oxygen species production but not ARG1 release.…”

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confidence: 99%