Lipidomic analysis of adipose-derived extracellular vesicles reveals specific EV lipid sorting informative of the obesity metabolic state

Blandin, Alexia; Dugail, Isabelle; Hilairet, Grégory; Ponnaiah, Maharajah; Ghesquière, Valentine; Froger, Josy; Ducheix, Simon; Fizanne, Lionel; Boursier, Jérôme; Cariou, Bertrand; Lhomme, Marie; Lay, Soazig Le

doi:10.1016/j.celrep.2023.112169

Cited by 28 publications

(34 citation statements)

References 49 publications

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“…The present study supports previous data showing that VD can modulate lipid composition of EVs from neuronal cell line and that inflammation also affects AdEVs. [27,31] In accordance with previous study, [32] the content of EVs in phospholipids, sphingolipids, glycerolipids, and lysophospholipids was confirmed in our study. Interestingly, these results are consistent with a recent study using two different obesity mice models that showed the same proportions of lipid classes in each lipid group (glycerolipids, phospholipids, sphingolipids, and lysophospholipids).…”

Section: Discussionsupporting

confidence: 93%

“…This observation is consistent with recent studies showing that TG are actively transported to target cells. [ 27,37 ] In accordance with this observation, it is widely known that adipocytes store a large quantity of TGs and that inflammation‐related obesity is associated to lipid accumulation in adipocytes. [ 38 ] In addition, we observed that the TG content of EVs was highly modulated by TNFα with seven and two TGs being significantly enhanced in sEVs and lEVs, respectively.…”

Section: Discussionmentioning

confidence: 62%

“…Interestingly, these results are consistent with a recent study using two different obesity mice models that showed the same proportions of lipid classes in each lipid group (glycerolipids, phospholipids, sphingolipids, and lysophospholipids). [ 27 ] We found different lipid profiles in sEVs and lEVs. The sEVs contained more Cer and SM, supporting previous studies showing that exosomes have a high proportion of sphingolipids.…”

Section: Discussionmentioning

confidence: 92%

“…The present study supports previous data showing that VD can modulate lipid composition of EVs from neuronal cell line and that inflammation also affects AdEVs. [ 27,31 ]…”

Section: Discussionmentioning

confidence: 99%

“…In accordance with this hypothesis, Blandin et al identified specific lipids enriched in AdEVs of various obese mouse models, suggesting that these lipids may be biomarkers or mediators of metabolic disorders. [27] Lipids are also important for the release of AdEV contents in target cells. For example, choles-terol content highly modulates the fusion of EVs with the cell membrane, [28,29] but other lipids such as phosphatidylserine (PS) are also involved.…”

Section: Introductionmentioning

confidence: 99%

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Vitamin D Modulates Lipid Composition of Adipocyte‐Derived Extracellular Vesicles Under Inflammatory Conditions

Payet,

Valmori,

Astier

et al. 2023

Molecular Nutrition Food Res

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Scope: Adipocyte‐derived extracellular vesicles (AdEVs) convey lipids that can play a role in the energy homeostasis. Vitamin D (VD) has been shown to limit the metabolic inflammation as it decreases inflammatory markers expression in adipose tissue (AT). However, VD effect on adipocytes‐derived EVs has never been investigated.Methods and results: Thus, the aim of this study is to evaluate the AdEVs lipid composition by LC‐MS/MS approach in 3T3‐L1 cells treated with VD or/and pro‐inflammatory factor (tumor necrosis factor α [TNFα]). Among all lipid species, four are highlighted (glycerolipids, phospholipids, lysophospholipids, and sphingolipids) with a differential content between small (sEVs) and large EVs (lEVs). This study also observes that VD alone modulates EV lipid species involved in membrane fluidity and in the budding of membrane. EVs treated with VD under inflammatory conditions have different lipid profiles than the control group, which is more pronounced in lEVs. Indeed, 25 lipid species are significantly modulated in lEVs, compared with only seven lipid species in sEVs.Conclusions: This study concludes that VD, alone or under inflammatory conditions, is associated with specific lipidomic signature of sEVs and lEVs. These observations reinforce current knowledge on the anti‐inflammatory effect of VD.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 92%

“…The present study supports previous data showing that VD can modulate lipid composition of EVs from neuronal cell line and that inflammation also affects AdEVs. [ 27,31 ]…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Vitamin D Modulates Lipid Composition of Adipocyte‐Derived Extracellular Vesicles Under Inflammatory Conditions

Payet,

Valmori,

Astier

et al. 2023

Molecular Nutrition Food Res

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Clinical Translation of Extracellular Vesicles

Ghodasara,

Raza,

Wolfram

et al. 2023

Adv Healthcare Materials

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Extracellular vesicles (EVs) occur in a variety of bodily fluids and have gained recent attraction as natural materials due to their bioactive surfaces, internal cargo, and role in intercellular communication. EVs contain various biomolecules, including surface and cytoplasmic proteins; and nucleic acids that are often representative of the originating cells. EVs can transfer content to other cells, a process that is thought to be important for several biological processes, including immune responses, oncogenesis, and angiogenesis. An increased understanding of the underlying mechanisms of EV biogenesis, composition, and function has led to an exponential increase in preclinical and clinical assessment of EVs for biomedical applications, such as diagnostics and drug delivery. Bacterium‐derived EV vaccines have been in clinical use for decades and a few EV‐based diagnostic assays regulated under Clinical Laboratory Improvement Amendments have been approved for use in single laboratories. Though, EV‐based products are yet to receive widespread clinical approval from national regulatory agencies such as the United States Food and Drug Administration (USFDA) and European Medicine Agency (EMA), many are in late‐stage clinical trials. This perspective sheds light on the unique characteristics of EVs, highlighting current clinical trends, emerging applications, challenges and future perspectives of EVs in clinical use.

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High‐fat diets: You are what you eat….your extracellular vesicles too!

Rome,

Tacconi

2023

J of Extracellular Vesicle

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Recent works indicate that the lipid composition of extracellular vesicles (EVs) can modify their biological functions and their incorporation into recipient cells. In particular high‐fat diets affect EV biogenesis, EV lipid composition, EV targeting and consequently the cross‐talk between tissues. This review connects different research topics to show that a vicious circle is established during the development of high‐fat diet‐induced obesity, connecting the alteration of lipid metabolism, the composition of extracellular vesicles and the spread of deleterious lipids between tissues, which participates in NAFLD/NASH and diabetes development. According to the studies described in this review, it is urgent to take an interest in this question as the modulation of EV lipid composition could be an important factor to take into account during the therapeutic management of patients suffering from metabolic syndrome and related pathologies such as obesity and diabetes. Furthermore, as lipid modification of EVs is a strategy currently being tested to enable better integration into their target tissue or cell, it is important to consider the impact of these lipid modifications on the homeostasis of these targets.

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Lipidomic analysis of adipose-derived extracellular vesicles reveals specific EV lipid sorting informative of the obesity metabolic state

Cited by 28 publications

References 49 publications

Vitamin D Modulates Lipid Composition of Adipocyte‐Derived Extracellular Vesicles Under Inflammatory Conditions

Vitamin D Modulates Lipid Composition of Adipocyte‐Derived Extracellular Vesicles Under Inflammatory Conditions

Clinical Translation of Extracellular Vesicles

High‐fat diets: You are what you eat….your extracellular vesicles too!

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