Vitamin D Modulates Lipid Composition of Adipocyte‐Derived Extracellular Vesicles Under Inflammatory Conditions

Payet, Thomas; Valmori, Marie; Astier, Julien; Svilar, Ljubica; Sicard, Flavie; Tardivel, Catherine; Ghossoub, Rania; Martin, Jean‐Charles; Landrier, Jean‐François; Mounien, Lourdes

doi:10.1002/mnfr.202300374

Cited by 3 publications

(1 citation statement)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, vitamin D affects the lipid composition (including SL) of adipocytederived extracellular vesicles, playing key roles in energy homeostasis [49]. 1,25(OH)D boosts TNF-a expression (apoptosis inducer), which raises ceramide (apoptosis inducer) levels via SM hydrolysis [28].…”

Section: Vitamin D and Sphingolipids Crosstalk: Preclinical Evidencementioning

confidence: 99%

Interplay between Vitamin D and Sphingolipids in Cardiometabolic Diseases

Fenizia,

Gaggini,

Vassalle

2023

IJMS

View full text Add to dashboard Cite

Sphingolipids (SLs) are structural, bioactive molecules with several key cellular roles, whereas 1,25-dihydroxyvitamin D (1,25(OH)D), the active form of vitamin D, is considered the major regulator of calcium homeostasis, although it also exerts other extraskeletal effects. Many studies reported the physiological connection between vitamin D and SLs, highlighting not only the effects of vitamin D on SL metabolism and signaling but also the influence of SLs on vitamin D levels and function, thus strongly suggesting a crosstalk between these molecules. After a brief description of 1,25(OH)D and SL metabolism, this review aims to discuss the preclinical and clinical evidence on the crosstalk between SLs and 1,25(OH)D, with a special focus on cardiometabolic diseases.

show abstract

Section: Vitamin D and Sphingolipids Crosstalk: Preclinical Evidencementioning

confidence: 99%

Interplay between Vitamin D and Sphingolipids in Cardiometabolic Diseases

Fenizia,

Gaggini,

Vassalle

2023

IJMS

View full text Add to dashboard Cite

show abstract

Genomic and non-genomic action of vitamin D on ion channels – Targeting mitochondria

Olszewska,

Zmijewski

2024

Mitochondrion

View full text Add to dashboard Cite

Increased secretion of adipocyte-derived extracellular vesicles is associated with adipose tissue inflammation and the mobilization of excess lipid in human obesity

Matilainen,

Berg,

Vaittinen

et al. 2024

J Transl Med

View full text Add to dashboard Cite

Background Obesity is a worldwide epidemic characterized by adipose tissue (AT) inflammation. AT is also a source of extracellular vesicles (EVs) that have recently been implicated in disorders related to metabolic syndrome. However, our understanding of mechanistic aspect of obesity’s impact on EV secretion from human AT remains limited. Methods We investigated EVs from human Simpson Golabi Behmel Syndrome (SGBS) adipocytes, and from AT as well as plasma of subjects undergoing bariatric surgery. SGBS cells were treated with TNFα, palmitic acid, and eicosapentaenoic acid. Various analyses, including nanoparticle tracking analysis, electron microscopy, high-resolution confocal microscopy, and gas chromatography–mass spectrometry, were utilized to study EVs. Plasma EVs were analyzed with imaging flow cytometry. Results EVs from mature SGBS cells differed significantly in size and quantity compared to preadipocytes, disagreeing with previous findings in mouse adipocytes and indicating that adipogenesis promotes EV secretion in human adipocytes. Inflammatory stimuli also induced EV secretion, and altered EV fatty acid (FA) profiles more than those of cells, suggesting the role of EVs as rapid responders to metabolic shifts. Visceral AT (VAT) exhibited higher EV secretion compared to subcutaneous AT (SAT), with VAT EV counts positively correlating with plasma triacylglycerol (TAG) levels. Notably, the plasma EVs of subjects with obesity contained a higher number of adiponectin-positive EVs than those of lean subjects, further demonstrating higher AT EV secretion in obesity. Moreover, plasma EV counts of people with obesity positively correlated with body mass index and TNF expression in SAT, connecting increased EV secretion with AT expansion and inflammation. Finally, EVs from SGBS adipocytes and AT contained TAGs, and EV secretion increased despite signs of less active lipolytic pathways, indicating that AT EVs could be involved in the mobilization of excess lipids into circulation. Conclusions We are the first to provide detailed FA profiles of human AT EVs. We report that AT EV secretion increases in human obesity, implicating their role in TAG transport and association with adverse metabolic parameters, thereby emphasizing their role in metabolic disorders. These findings promote our understanding of the roles that EVs play in human AT biology and metabolic disorders.

show abstract

Vitamin D Modulates Lipid Composition of Adipocyte‐Derived Extracellular Vesicles Under Inflammatory Conditions

Cited by 3 publications

References 63 publications

Interplay between Vitamin D and Sphingolipids in Cardiometabolic Diseases

Interplay between Vitamin D and Sphingolipids in Cardiometabolic Diseases

Genomic and non-genomic action of vitamin D on ion channels – Targeting mitochondria

Increased secretion of adipocyte-derived extracellular vesicles is associated with adipose tissue inflammation and the mobilization of excess lipid in human obesity

Contact Info

Product

Resources

About