Lipid Readjustment in Yarrowia lipolytica Odd-Chain Fatty Acids Producing Strains

Abreu, Sonia; Park, Young Kyoung; Souza, Camilla Pires de; Vidal, Léa; Chaminade, Pierre; Nicaud, Jean‐Marc

doi:10.3390/biom12081026

Cited by 3 publications

(2 citation statements)

References 59 publications

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“…High unsaturation levels with carbon chains of up to 20 carbon atoms have been observed in the MGDGs of non‐green algae (Sato et al, 2003). Odd‐chain fatty acids are unusual lipids that have been observed in several organisms, including plants (Rodríguez‐López et al, 2017), algae (Chang et al, 2011; Santiago‐Vázquez & Jacobs, 2005; Zhang et al, 2020), yeast (Abreu et al, 2022), and bacteria (Bhatia et al, 2019). Saturated OCFAs, such as pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0), provide various health benefits, such as improvements in glucose metabolism and inflammatory activities (Kurotani et al, 2017; Venn‐Watson et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Characterization of monogalactosyldiacylglycerol synthases in Gracilariopsis lemaneiformis and their potential roles in the fading of the thallus

Zhang,

Yuan,

et al. 2023

Journal of Phycology

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Membrane lipids play essential roles in regulating physiological properties in higher plants and algae. Monogalactosyldiacylglycerol (MGDG) is a major thylakoid membrane lipid, and it is an important source of polyunsaturated fatty acids for cells, plays a key role in the biogenesis of plastids, and maintains the function of the photosynthetic machinery. Several studies have indicated that the knockdown of MGDG synthase results in membrane lipid remodeling, albino seedlings, and changes in photosynthetic performance. However, the effects of MGDG synthase (MGD) inhibitors on lipids in macroalgae have not yet been clarified. Here, we characterized the effects of MGD inhibitors (ortho‐phenanthroline and N‐ethylmaleimide) on the composition of the fatty acids observed in MGDG and digalactosyldiacylglycerol (DGDG) in Gracilariopsis lemaneiformis using electrospray ionization–mass spectrometry. The most abundant MGDG species contained 16:0/18:1 (sn‐1/sn‐2) fatty acids, and the most dominant DGDG species contained 20:5/16:0 (sn‐1/sn‐2) fatty acids. Measurements of photosynthetic pigments and photosynthetic parameters revealed that photosynthesis of G. lemaneiformis was impaired. Principal component analysis and Spearman's correlation analysis revealed interactions between specific MGDG structural composition patterns and key metabolites involved in photosynthesis, indicating that 20:4/16:0 (sn‐1/sn‐2) MGDG and 16:0/18:1 (sn‐1/sn‐2) MGDG affect the structure and function of phycobilisomes and thus the color of G. lemaneiformis. Three genes (GlMGD1, GlMGD2, and GlMGD3) were cloned and identified. The addition of N‐ethylmaleimide to G. lemaneiformis did not affect the abundance of GlMGD mRNA, and the abundance of transcripts was significantly decreased by ortho‐phenanthroline.

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Section: Discussionmentioning

confidence: 99%

Characterization of monogalactosyldiacylglycerol synthases in Gracilariopsis lemaneiformis and their potential roles in the fading of the thallus

Zhang,

Yuan,

et al. 2023

Journal of Phycology

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“…Microorganisms have evolved a set of mechanisms to deal with harsh environments after exposure to nutrientlimited conditions (Ratledge and Wynn, 2002;Ratledge, 2004). When microorganisms grow in a medium with unbalanced nutritional conditions, especially a nitrogenlimited medium with an excess of carbon, the signal of the harsh environment initiates the development of a mechanism to absorb more nutrients to cope with severe conditions (Abreu et al, 2022). Oleaginous microorganisms usually absorb chemical units richer in carbon content, which is stored in the formation of fatty acids for future use in hostile environments to which they may be exposed (Liu et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

The Enzymatic Role of Lipid Metabolism in Yarrowia lipolytica Grown on Glycerol

Xin¹,

Chang²,

Dang³

et al. 2023

American Journal of Biochemistry and Biotechnology

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More attention has been paid to the production of commercial fatty acids by microbial fermentation. The basic biochemistry of lipid accumulation in oleaginous microorganisms under nitrogen limitation has been extensively studied. As an oleaginous microorganism, Yarrowia lipolytica can produce fatty acid-derived biofuels and biochemicals. However, the response of the enzymes in Y. lipolytica to glycerol remains unclear. Therefore, we aimed to identify the rate-limiting enzymes associated with the mechanism of lipid accumulation of Y. lipolytica grown on glycerol. The variations in key enzyme activities of fatty acid accumulation in Y. lipolytica were investigated with glycerol as the single carbon source. The nitrogen source was exhausted at 10 h in the medium, leading to substantial changes in key enzyme activities associated with lipid accumulation. Adenosine monophosphate deaminase activity increased immediately by approximately twofold. NAD + -isocitrate dehydrogenase activity decreased by 65% after the nitrogen source was exhausted when compared to the initial maximum activity. ATP citrate lyase activity, which provides the substrate acetyl coenzyme A for lipid biosynthesis, increased by approximately fourfold when compared to the activity before nitrogen depletion. Malic Enzyme (ME) activity decreased considerably after nitrogen was exhausted so that the reducing power Nicotinamide Adenine Dinucleotide Phosphate (NADPH) required for lipid synthesis cannot be produced by ME. However, glucose-6-phosphate dehydrogenase activity increased from 550 nmol•min -1 •mg -1 -771 nmol•min -1 •mg -1 , suggesting that the main source of NADPH required for fatty acid accumulation may be provided by the pentose phosphate pathway when Y. lipolytica grew on the medium with glycerol as the only carbon source.

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Pilot Scale Production of Single Cell Oil by Apiotrichum brassicae and Pichia kudriavzevii from Acetic Acid and Propionic Acid

Burgstaller

Oliver²,

Dietrich³

et al. 2023

Applied Sciences

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Volatile fatty acids can be used as a cheap carbon source for biotechnological lipid production with oleaginous yeasts, but one factor limiting their large-scale use is their inherent cytotoxicity. Developing a suitable cultivation strategy can help mitigate the adverse effect volatile fatty acids have, since these effects are strongly dependent on concentration and pH. This work shows that, by employing a pH-stat fed-batch approach for the cultivation of Apiotrichum brassicae V134 and Pichia kudriavzevii V194, lipid contents above 56 g/100 g dry cell weight and dry cell weight concentrations above 30 g/L can be reached. Furthermore, volumetric lipid productivities up to 0.29 g/Lh could be achieved using acetic and propionic acid as a sole carbon source. It was also demonstrated that the developed process is robust and scalable. Scale-up to the 500 L scale resulted in a similar lipid yield, dry cell weight (31–37 g/L), and single cell oil content (56 g/100 g dry cell weight–58 g/100 g dry cell weight). The main fatty acid present in the produced lipids was oleic acid (36–43%), but also odd-numbered fatty acids, especially heptadecanoic acid (7–15%), were present. Additionally, different methods for the pretreatment of biomass prior to lipid extraction were assessed, and the iodine value (48), peroxide value (7.3), and acid value (4.3) of the extracted single cell oil were determined.

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Lipid Readjustment in Yarrowia lipolytica Odd-Chain Fatty Acids Producing Strains

Cited by 3 publications

References 59 publications

Characterization of monogalactosyldiacylglycerol synthases in Gracilariopsis lemaneiformis and their potential roles in the fading of the thallus

Characterization of monogalactosyldiacylglycerol synthases in Gracilariopsis lemaneiformis and their potential roles in the fading of the thallus

The Enzymatic Role of Lipid Metabolism in Yarrowia lipolytica Grown on Glycerol

Pilot Scale Production of Single Cell Oil by Apiotrichum brassicae and Pichia kudriavzevii from Acetic Acid and Propionic Acid

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