Pilot Scale Production of Single Cell Oil by Apiotrichum brassicae and Pichia kudriavzevii from Acetic Acid and Propionic Acid

Abstract

Volatile fatty acids can be used as a cheap carbon source for biotechnological lipid production with oleaginous yeasts, but one factor limiting their large-scale use is their inherent cytotoxicity. Developing a suitable cultivation strategy can help mitigate the adverse effect volatile fatty acids have, since these effects are strongly dependent on concentration and pH. This work shows that, by employing a pH-stat fed-batch approach for the cultivation of Apiotrichum brassicae V134 and Pichia kudriavzevii V194, lipid contents above 56 g/100 g dry cell weight and dry cell weight concentrations above 30 g/L can be reached. Furthermore, volumetric lipid productivities up to 0.29 g/Lh could be achieved using acetic and propionic acid as a sole carbon source. It was also demonstrated that the developed process is robust and scalable. Scale-up to the 500 L scale resulted in a similar lipid yield, dry cell weight (31–37 g/L), and single cell oil content (56 g/100 g dry cell weight–58 g/100 g dry cell weight). The main fatty acid present in the produced lipids was oleic acid (36–43%), but also odd-numbered fatty acids, especially heptadecanoic acid (7–15%), were present. Additionally, different methods for the pretreatment of biomass prior to lipid extraction were assessed, and the iodine value (48), peroxide value (7.3), and acid value (4.3) of the extracted single cell oil were determined.

Production of Single-Cell Oil from a Local Isolate Bacillus subtilis Using Palm Fronds

“…Cianchetta et al [ 16 ] concluded that linoleic acid and stearic acid are among the most important fatty acid constituents of the unicellular oil produced from Cutaneotrichosporon oleaginosum , Rhodosporidiobolus azoricus , and Lipomyces starkeyi . Khaleel and Fakhri [ 60 ] obtained SCO with high linoleic acid and stearic acid content, while Burgstaller et al [ 61 ] noted that the main fatty acid present in the oils produced from Apiotrichum brassicae and Pichia kudriavzevii was oleic acid.…”

Effects of yeast culture on in vitro ruminal fermentation and microbial community of high concentrate diet in sheep

“…On the contrary, the YC2 group displayed reduced Apiotrichum relative abundance when compared to CON group. The Apiotrichum has lipolytic activity (Burgstaller et al 2023 ), and the decreased Apiotrichum relative abundance may protect lipid from degradation in the rumen and can be used in the small intestine. Furthermore, obvious differences of unclassified_p__Ascomycota , Microascus , Ascochyta , Vishniacozyma , Neosetophoma and Sterigmatomyces relative abundances were observed among different treatments.…”