Lipid production from sweet sorghum bagasse through yeast fermentation

Liang, Yanna; Tang, Tianyu; Siddaramu, Thara; Choudhary, Ruplal; Umagiliyage, Arosha Loku

doi:10.1016/j.renene.2011.09.035

Cited by 77 publications

(26 citation statements)

References 41 publications

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“…Thus, the two-staged culture mode was efficient to convert glucose into lipids by C. curvatus . It should also be noted that sugar consumption rate of the two-staged culture mode was significantly higher than that of the single-staged mode [7]. …”

Section: Resultsmentioning

confidence: 99%

“…Cryptococcus curvatus is an excellent lipid producer. It can use various cheap substrates such as biomass hydrolysates and has a good tolerance to major biomass-derived inhibitors [5,7,14]. In this paper, we describe the simultaneous saccharification and enhanced lipid production (SSELP) process that integrates the biomass hydrolysis step and an enhanced lipid accumulation step, to effectively convert lignocellulosic materials into lipids.…”

Section: Introductionmentioning

confidence: 99%

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Efficient conversion of biomass into lipids by using the simultaneous saccharification and enhanced lipid production process

Gong

Shen

Wang

et al. 2013

Biotechnol Biofuels

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BackgroundMicrobial lipid production by using lignocellulosic biomass as the feedstock holds a great promise for biodiesel production and biorefinery. This usually involves hydrolysis of biomass into sugar-rich hydrolysates, which are then used by oleaginous microorganisms as the carbon and energy sources to produce lipids. However, the costs of microbial lipids remain prohibitively high for commercialization. More efficient and integrated processes are pivotal for better techno-economics of microbial lipid technology.ResultsHere we describe the simultaneous saccharification and enhanced lipid production (SSELP) process that is highly advantageous in terms of converting cellulosic materials into lipids, as it integrates cellulose biomass hydrolysis and lipid biosynthesis. Specifically, Cryptococcus curvatus cells prepared in a nutrient-rich medium were inoculated at high dosage for lipid production in biomass suspension in the presence of hydrolytic enzymes without auxiliary nutrients. When cellulose was loaded at 32.3 g/L, cellulose conversion, cell mass, lipid content and lipid coefficient reached 98.5%, 12.4 g/L, 59.9% and 204 mg/g, respectively. Lipid yields of the SSELP process were higher than those obtained by using the conventional process where cellulose was hydrolyzed separately. When ionic liquid pretreated corn stover was used, both cellulose and hemicellulose were consumed simultaneously. No xylose was accumulated over time, indicating that glucose effect was circumvented. The lipid yield reached 112 mg/g regenerated corn stover. This process could be performed without sterilization because of the absence of auxiliary nutrients for bacterial contamination.ConclusionsThe SSELP process facilitates direct conversion of both cellulose and hemicellulose of lignocellulosic materials into microbial lipids. It greatly reduces time and capital costs while improves lipid coefficient. Optimization of the SSELP process at different levels should further improve the efficiency of microbial lipid technology, which in turn, promote the biotechnological production of fatty acid-derived products from lignocellulosic biomass.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficient conversion of biomass into lipids by using the simultaneous saccharification and enhanced lipid production process

Gong

Shen

Wang

et al. 2013

Biotechnol Biofuels

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“…The same sorghum bagasse and corn fiber that were studied in our previous works [4,[22][23][24]35] were used in this research. Based on National Renewable Energy Laboratory (NREL)'s protocol [33], the sorghum bagasse contained approximately 36.9±1.6 % cellulose, 17.8± 0.6 % hemicellulose, and 19.5±1.1 % lignin [4].…”

Section: Pretreatment Of Sorghum Bagasse and Corn Fibermentioning

confidence: 99%

Sophorolipid Production from Biomass Hydrolysates

Samad

Zhang

Chen

et al. 2014

Appl Biochem Biotechnol

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Although extensive research has been conducted on producing sophorolipids using Candida (Starmerella) bombicola from pure sugars and various oil sources, production of this biosurfactant has not been evaluated when cells are cultivated in lignocellulosic hydrolysates. Here, we report for the first time that C. bombicola is capable of producing sophorolipids on hydrolysates derived from sweet sorghum bagasse and corn fiber. Without oil supplementation, a sophorolipid concentration of 3.6 and 1.0 g/L was detected from cultures with bagasse and corn fiber hydrolysates, respectively. With the addition of soybean oil at 100 g/L, the yield of sophorolipids from these two hydrolysates in the same order was 84.6 and 15.6 g/L. Surprisingly, C. bombicola consumed all monomeric sugars and nonsugar compounds in the hydrolysates, and cultures with bagasse hydrolysates had higher yield of sophorolipids than those from a standard medium which contained pure glucose at the same concentration.

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“…Lowvalued nutrient sources are often challenging to utilize due to their heterogeneous nature and the presence of inhibitory compounds. Oleaginous yeasts have been shown to be versatile biocatalysts able to utilize a variety of alternative nutrient sources to produce SCO which has been recently reviewed by Leiva-Candia et al (2014) and Huang et al (2013).Some examples include: animal fat , corn cobs (Huang et al, 2012;Liang et al, 2014), corn fiber (Galafassi et al, 2012), corn stover , cheese whey (Seo et al, 2014), crude glycerol (Papanikolaou & Aggelis, 2002;Thiru et al, 2011), flourrich waste streams (Tsakona et al, 2014), molasses (Zhu et al, 2008), monosodium glutamate wastewater , rapeseed meal (Kiran et al, 2012), rice straw (Huang et al, 2009), sewage sludge (Angerbauer et al, 2008), sorghum bagasse (Liang et al, 2012), sugarcane bagasse (Huang et al, 2012a;Tsigie, 2011), volatile FFA (Zhan et al, 2013), wheat straw (Yu et al, 2011) and spent yeast lysates (Espinosa-Gonzalez et al, 2014;Thiru et al, 2011;Yang et al, 2014). Low-value, plant-based lignocellulosic feedstocks have the potential to serve as low-cost sources of carbon for SCO production.…”

Section: Nutrient Sourcesmentioning

confidence: 99%

Oleaginous yeast: a value-added platform for renewable oils

Probst

Schulte²,

Durrett

et al. 2015

Critical Reviews in Biotechnology

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Yeast single cell oil (SCO) is a non-crop-based, renewable oil source that can be used for the production of bio-based oleochemicals. Stand-alone production of SCO for oleochemicals is currently not cost-competitive because lower-cost alternatives from petroleum and crop-based resources are available. Utilizing low-valued nutrient sources, implementing cost-efficient downstream processes and adopting biotechnological advancements such as systems biology and metabolic engineering could prove valuable in making an SCO platform a reality in the emerging bio-based economy. This review aims to consider key biochemical pathways for storage lipid synthesis, possible pathways for SCO yield improvement, previously used bioprocessing techniques for SCO production, challenges in SCO commercialization and advantages of adopting a renewable SCO platform.

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Lipid production from sweet sorghum bagasse through yeast fermentation

Cited by 77 publications

References 41 publications

Efficient conversion of biomass into lipids by using the simultaneous saccharification and enhanced lipid production process

Efficient conversion of biomass into lipids by using the simultaneous saccharification and enhanced lipid production process

Sophorolipid Production from Biomass Hydrolysates

Oleaginous yeast: a value-added platform for renewable oils

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