Yeast single cell oil (SCO) is a non-crop-based, renewable oil source that can be used for the production of bio-based oleochemicals. Stand-alone production of SCO for oleochemicals is currently not cost-competitive because lower-cost alternatives from petroleum and crop-based resources are available. Utilizing low-valued nutrient sources, implementing cost-efficient downstream processes and adopting biotechnological advancements such as systems biology and metabolic engineering could prove valuable in making an SCO platform a reality in the emerging bio-based economy. This review aims to consider key biochemical pathways for storage lipid synthesis, possible pathways for SCO yield improvement, previously used bioprocessing techniques for SCO production, challenges in SCO commercialization and advantages of adopting a renewable SCO platform.
A flavour model system was encapsulated by spray-drying, using traditional carrier materials, and then subjected to agglomeration by fluidized bed processing. Flavour retention was not adversely affected by the secondary processing as long as agglomeration did not promote structure collapse. Agglomerated powders showed higher moisture contents, absolute densities and mean particle sizes than spray-dried-only powders. Surface oil decreased in the agglomerated powders as a consequence of the stripping effect in the agglomerator, but this reduction was not necessarily correlated with better shelf-life. In general, agglomeration did not considerably change the properties of the powders beyond the limitations inherently associated with the carriers employed.
Single cell oil (SCO) is a valuable noncrop-based renewable oil source. Hemicellulose derived sugars can be utilized to produce SCO using the oleaginous yeast Lipomyces starkeyi ATCC 56304. Bran by-products were tested as hemicellulose-rich feedstocks for the production of SCO. Whole and destarched corn and wheat bran hydrolysates were produced using hydrothermal and dilute sulfuric acid (0%, 0.5%, 1.0%, v/v) pretreatment along with enzymatic hydrolysis. Whole bran hydrolysates produced from hydrothermal pretreatment generated the highest average oil yields of 126.7 and 124.3 mg oil/g sugar for both wheat and corn bran, respectively. 1.0% acid pretreatment was effective for the destarched bran generating a hemicellulose hydrolysis efficiency of 94% and 84% for wheat and corn bran, respectively, resulting in the highest oil yield of 70.7 mg oil/g sugar. The results indicate pretreated corn and wheat bran hydrolysates can serve as viable feedstocks for oleaginous yeast SCO bioconversion.
Liquid–liquid
equilibria data were determined for four ternary
systems of water + alcohol + methoxycyclopentane (CPME); the alcohols
used were methanol, ethanol, 1-propanol, and 2-propanol. All measurements
were performed at 298.15 K and atmospheric pressure. Binodal curves
were experimentally determined using both the cloud-point method and
tie lines. The reliability of the tie-line data were verified by the
Hand and Othmer–Tobias plots. Distribution coefficients and
separation factors were determined from the tie-line data. The universal
quasichemical activity coefficient model was used to correlate the
experimental data. Additionally, these water–alcohol–CPME
systems were compared to the water–methanol–chloroform
system as a green alternative for Bligh and Dyer lipid extraction.
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