Oleaginous yeast: a value-added platform for renewable oils

Probst, Kyle V.; Schulte, Leslie R.; Durrett, Timothy P.; Rezac, Mary E.; Vadlani, Praveen V.

doi:10.3109/07388551.2015.1064855

Cited by 91 publications

(66 citation statements)

References 132 publications

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“…Therefore, metabolic engineering is necessary to improve lipid productivity. Additionally, the production of other value-added co-products and exploration of zero-cost waste or by-product streams such as glycerol, as feedstock, for yeast SCO production is recommended [6]. Synthetic defined media containing 6.7 g/L Yeast Nitrogen Base (YNB) w/ammonium sulfate w/o amino acids (Becton, Dickson, and company), 20 g/L glucose, and a drop-out synthetic mix minus uracil (-Ura) or minus leucine (-Leu) (US Biological) were used for the selection of knock out/in strains.…”

Section: Introductionmentioning

confidence: 99%

Engineering Yarrowia lipolytica for Enhanced Production of Lipid and Citric Acid

Abghari

Chen

2017

Fermentation

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Increasing demand for plant oil for food, feed, and fuel production has led to food-fuel competition, higher plant lipid cost, and more need for agricultural land. On the other hand, the growing global production of biodiesel has increased the production of glycerol as a by-product. Efficient utilization of this by-product can reduce biodiesel production costs. We engineered Yarrowia lipolytica (Y. lipolytica) at various metabolic levels of lipid biosynthesis, degradation, and regulation for enhanced lipid and citric acid production. We used a one-step double gene knock-in and site-specific gene knock-out strategy. The resulting final strain combines the overexpression of homologous DGA1 and DGA2 in a POX-deleted background, and deletion of the SNF1 lipid regulator. This increased lipid and citric acid production in the strain under nitrogen-limiting conditions (C/N molar ratio of 60). The engineered strain constitutively accumulated lipid at a titer of more than 4.8 g/L with a lipid content of 53% of dry cell weight (DCW). The secreted citric acid reached a yield of 0.75 g/g (up to~45 g/L) from pure glycerol in 3 days of batch fermentation using a 1-L bioreactor. This yeast cell factory was capable of simultaneous lipid accumulation and citric acid secretion. It can be used in fed-batch or continuous bioprocessing for citric acid recovery from the supernatant, along with lipid extraction from the harvested biomass.

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Section: Introductionmentioning

confidence: 99%

Engineering Yarrowia lipolytica for Enhanced Production of Lipid and Citric Acid

Abghari

Chen

2017

Fermentation

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“…New bioremediation technologies can help to solve wastewater pollution problems and produce valuable biotechnological products. Microbial oil is defined as the accumulation of neutral storage lipids by oleaginous microorganisms . Microbial oils are renewable sources for the production of biodiesel feedstocks and polyunsaturated fatty acids (PUFAs) …”

Section: Introductionmentioning

confidence: 99%

Biovalorization of vegetable oil refinery wastewater into value‐added compounds by Yarrowia lipolytica

Darvishi

Salmani

Hosseini

2019

J of Chemical Tech & Biotech

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BACKGROUND Vegetable oil refinery wastewater (VORW) is an environmental pollutant in less developed countries which annually produce 75% of vegetable oils worldwide. Microbial oils from low‐cost substrates are of growing importance for the production of renewable biofuel feedstocks, and food and feed supplements. In the current study, VORW was used as a cheap substrate to produce added‐value compounds including lipase, microbial oil and lipid‐rich biomass using Yarrowia lipolytica. The chemical oxygen demand (COD) reduction of the wastewater was studied after fermentation. RESULTS The maximum microbial oil concentration and lipase activity were 2.68 g L−1 and 8 U mL−1 after 48 h of inoculation. The microbial oil production increased from 2.86 g L−1 to 7.74 g L−1 after optimization by response surface methodology. Using optimized medium, 11 g L−1 microbial oil and 18.3 g L−1 biomass were obtained in the bioreactor culture. The amount of unsaturated fatty acids (FAs) reached ≤63% in the resulting microbial oil with a suitable FA profile for the production of lipid‐rich biomass. The biomass of Y. lipolytica has 60% lipid content and a significant amount of essential FAs which makes it as a proper feed supplement. The COD level of the wastewater decreased by 80% after 20 h. CONCLUSION VORW can be used as a promising substrate for the sustainable production of microbial oil and lipid‐rich biomass with useful FAs by Y. lipolytica. This process also can simultaneously reduce environmental pollution of wastewater in a safe and eco‐friendly way. © 2019 Society of Chemical Industry

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“…Single cell oil (SCO) is a renewable alternative to crop‐based oils for the production of biofuels and oleochemicals. Oleaginous yeast are advantageous SCO producers that are capable of accumulating oil up to ∼70% of the dry cell mass . Oil or storage lipid accumulation occurs under nutrient limited conditions.…”

Section: Introductionmentioning

confidence: 99%

“…Oleaginous yeast are advantageous SCO producers that are capable of accumulating oil up to 70% of the dry cell mass. 1 Oil or storage lipid accumulation occurs under nutrient limited conditions. Fermentation techniques used for yeast SCO production often control the C:N ratio by limiting the amount of nitrogen and providing excess carbon (often in the form of glucose or some other sugar substrate).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of green solvents: Oil extraction from oleaginous yeast Lipomyces starkeyi using cyclopentyl methyl ether (CPME)

Probst

Wales

Rezac

et al. 2017

Biotechnology Progress

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Cyclopentyl methyl ether (CPME) was evaluated for extracting oil or triacylglycerol (TAG) from wet cells of the oleaginous yeast Lipomyces starkeyi. CPME is a greener alternative to chloroform as a potential solvent for oil recovery. A monophasic system of CPME and biphasic system of CPME:water (1:0.7) performed poorly having the lowest TAG extraction efficiency and TAG selectivity compared to other monophasic systems of hexane and chloroform and the biphasic Bligh and Dyer method (chloroform:methanol:water). Biphasic systems of CPME:water:alcohol (methanol/ethanol/1-propanol) were tested and methanol achieved the best oil extraction efficiency compared to ethanol and 1-propanol. Different biphasic systems of CPME:methanol:water were tested, the best TAG extraction efficiency and TAG selectivity achieved was 9.9 mg/mL and 64.6%, respectively, using a starting ratio of 1:1.7:0.6 and a final ratio of 1:1:0.8 (CPME:methanol:water). Similar results were achieved for the Bligh and Dyer method (TAG extraction efficiency of 10.2 mg/mL and TAG selectivity of 66.0%) indicating that the biphasic CPME system was comparable. The fatty acid profile remained constant across all the solvent systems tested indicating that choice of solvent was not specific for any certain fatty acid. This study was able to demonstrate that CPME could be used as an alternative solvent for the extraction of oil from the wet biomass of oleaginous yeast. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1096-1103, 2017.

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Oleaginous yeast: a value-added platform for renewable oils

Cited by 91 publications

References 132 publications

Engineering Yarrowia lipolytica for Enhanced Production of Lipid and Citric Acid

Engineering Yarrowia lipolytica for Enhanced Production of Lipid and Citric Acid

Biovalorization of vegetable oil refinery wastewater into value‐added compounds by Yarrowia lipolytica

Evaluation of green solvents: Oil extraction from oleaginous yeast Lipomyces starkeyi using cyclopentyl methyl ether (CPME)

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