Lipid-free glycerol teichoic acids with potent membrane-binding activity

Cooper, H R; Chorpenning, Frank W.; Roses, S

doi:10.1128/iai.19.2.462-469.1978

Cited by 22 publications

(10 citation statements)

References 15 publications

(31 reference statements)

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“…By using 25 kg1150 p1 of our dLTA, no significant reduction of binding to MNC was observed, while with 2.5 pg/150 1 1 dLTA the binding to MNC was reduced by about 50%. Parallel to our results, Cooper et al (10) have shown erythrocyte-membrane binding activity of lipid-free glycerol teichoic acids.…”

Section: Resultssupporting

confidence: 92%

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THE MITOGENIC PROPERTIES OF LIPOTEICHOIC ACID FROM STAPHYLOCOCCUS AUREUS

Aasjord¹,

Nyland²,

Matre³

1986

Acta Pathologica Microbiologica Scandinavica Series C: Immunolo

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Aasjord, P., Nyland, H. & Matre, R. The mitogenic properties of lipoteichoic acid from Staphylococcus aureus. Acta path. microbiol. immunol. scand. Sect. C, 94: 91-96, 1986. Lipoteichoic acid (LTA) isolated from Staphylococcus aureus Cowan I induced an in vifro blastogenic response in isolated mononuclear cells (MNC). Seventeen of 20 normal donors responded strongly, with peak response after 3 -5 days of incubation. Significant stimulation was also obtained with human cord blood MNC from 3 donors, further indicating that the stimulation was due to a general mitogenic effect. The mitogenicity of deacylated LTA (dLTA) was similar to that of LTA. Binding of LTA to isolated MNC was demonstrated by an indirect immunofluorescent technique staining with specific rabbit anti-LTA antibodies and by rosette inhibition experiments. Results obtained in cell fraction experiments indicated that LTA had a monocyte dependent mitogenic effect on T lymphocytes.

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Section: Resultssupporting

confidence: 92%

“…( 1 I, 13, 20). The mitogenic properties of bacteria and their products depend on a number of factors, including bacterial species, method used for preparation of fractions and animal species in which they are studied (9,10,14).…”

Section: Resultsmentioning

confidence: 99%

THE MITOGENIC PROPERTIES OF LIPOTEICHOIC ACID FROM STAPHYLOCOCCUS AUREUS

Aasjord¹,

Nyland²,

Matre³

1986

Acta Pathologica Microbiologica Scandinavica Series C: Immunolo

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“…The lipid portion of the teichoic acids has been associated with a number of biological proper-ties (38,44). In particular there is some controversy regarding the importance of the covalently bound fatty acid for sensitizing RBCs for HA (8,31,44). The results of our studies (Table 3) suggest no direct quantitative relationship between either total or specific lipids and the ability to sensitize the RBCs.…”

Section: Hamentioning

confidence: 99%

Antigenic determinant of the Lancefield group H antigen of Streptococcus sanguis

Rosan¹,

Argenbright²

1982

Infect Immun

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Previous studies indicated that the teichoic acid isolated from strains of Streptococcus sanguis was group specific and defined the Lancefield group H streptococci. To determine the specific antigenic determinants, the antigen was extracted from a group H streptococcus (ATCC 903) by the phenol-water method and purified by column chromatography. The isolated antigen had a glycerol/ phosphate/glucose molar ratio of 1:0.9:0.3; the lipid concentration was 7.6% of its dry Weight. No nucleic acids were detected, and amino acids constituted approximately 2% of the dry weight. The minimum concentration of antigen required to sensitize erythrocytes for hemagglutination with a 1:1,P00 dilution of either group H antiserum or antiteichoic acid serum was 0.02 ,ug/ml. Hemaggluti-925 on July 16, 2020 by guest

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“…here to mammalian tissues has not been characterized, although a number of components of group A streptococci have been proposed as possible mediators. Of these, lipoteichoic acid (LTA) was reported to adsorb to membranes of epithelial cells (1,5,34), erythrocytes (7,12,27,34), lymphocytes (6), and heart and kidney cells (13) and to block the adherence of intact bacteria to a number of animal cells in vitro (1,13,34). Other observations, however, suggest that LTA may not be the principal bacterial polymer involved in adherence.…”

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confidence: 99%

Binding of streptococcal antigens to muscle tissue in vitro

Stinson¹,

Nisengard²,

Bergey³

1980

Infect Immun

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Antigens extracted from cells of Streptococcus pyogenes T6 and Streptococcus mutans strains AHT, BHT, 10449, OMZ175, and K1R adsorbed to the sarcolemmal sheath of cardiac muscle cells in vitro. Similar preparations from S. salivarius, S. sanguis, Staphylococcus aureus, and Lactobacillus casei had weak or negligible tissue-binding activity. Tissue-bound bacterial antigens were detected with homologous rabbit antisera with both indirect immunofluorescence tests and an indirect radioimmunoassay. Serological cross-reactivity was observed between the tissue-binding factors of S. pyogenes and S. mutans cells but not between the bacteria and muscle tissue. In a comparative study of extraction procedures, the greatest yield of tissue-binding factors was obtained from group A streptococci by cell disruption in buffer at 40C. Hot aqueous phenol and hot water extracts were inactive. Antibodies specific for the tissue-binding factor(s) were readily adsorbed from rabbit anti-S. pyogenes serum by a preparation of isolated cytoplasmic membranes but not by a suspension of cell wall fragments. The heart-binding component of S. pyogenes cell extracts was inactivated by protease digestion and heat treatment and to a lesser extent by periodic acid oxidation. The capacity of heart cell components to adsorb streptococcal antigens was reduced by protease treatment but not by the action of neuraminidase, hyaluronidase, organic solvents, or detergents. Streptococci have been shown to selectively adhere to epithelial cells (1-3, 8, 39), tooth surfaces (23, 24), and salivary components (11, 21, 22, 30) of the human oral cavity. These studies suggest that this phenomenon plays a fundamental role in the colonization of oral surfaces by these bacteria and, consequently, the pathogenesis of certain diseases including streptococcal pharyngitis and dental caries (for review, see 20, 24, 38). The mechanisms) by which streptococci ad-'Titers (IIF) from each of three rabbits immunized with the bacterium. S. aureus titer was determined by agglutination.

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Lipid-free glycerol teichoic acids with potent membrane-binding activity

Cited by 22 publications

References 15 publications

THE MITOGENIC PROPERTIES OF LIPOTEICHOIC ACID FROM STAPHYLOCOCCUS AUREUS

THE MITOGENIC PROPERTIES OF LIPOTEICHOIC ACID FROM STAPHYLOCOCCUS AUREUS

Antigenic determinant of the Lancefield group H antigen of Streptococcus sanguis

Binding of streptococcal antigens to muscle tissue in vitro

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