Antigenic determinant of the Lancefield group H antigen of Streptococcus sanguis

Rosan, Burton; Argenbright, Lois S.

doi:10.1128/iai.38.3.925-931.1982

Cited by 9 publications

(5 citation statements)

References 37 publications

(69 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Serology. Although Lancefield group H and S. sanguis are not synonymous (6,7,32), our strains were tested against group H antiserum to look for possible correlations between serological reactions and surface structure. The Wellcome group H antiserum reacted with 42% (16 of 36) of the S. sanguis I stains and with none of the S. sanguis II strains.…”

Section: Resultsmentioning

confidence: 99%

Surface structures (peritrichous fibrils and tufts of fibrils) found on Streptococcus sanguis strains may be related to their ability to coaggregate with other oral genera

Handley¹,

Carter²,

Wyatt³

et al. 1985

Infect Immun

131

View full text Add to dashboard Cite

We screened 36 strains of Streptococcus sanguis biotype I and 8 strains of S. sanguis biotype HI for the presence of surface structures and for their ability to coaggregate with Actinomyces viscosus, Actinomyces naeslundii, and Fusobacterium nucleatum. Negative staining under an electron microscope revealed detectable surface structures on all S. sanguis strains. The majority of strains (38 of 44) carried peritrichous fibrils, which have an irregular profile and no distinct width. They usually appeared as a fringe with a constant width around the cell. Strains selected for measurement had a fringe with an average length of 72.4 ± 8.5 nm on biotype I strains and 51.6 + 3.3 nm on biotype II strains. Some fibrillar biotype I strains carried an additional, longer (158.7 ± 33.1 nm) type of fibril projecting through the shorter fibrils. Fibrillar density was characteristic for each strain, ranging from very dense on all cells in a population to very sparse on a few cells in a population. A small group of six strains carried tufts of fibrils in a lateral or polar position on the cell. Either one or two lengths of fibril were present in the tuft depending on the strain. One strain carried both peritrichous fibrils and fimbriae. Fimbriae are flexible structures with a constant width (4.5 to 5.0 nm) all along their length but very variable lengths (.0.7 ,um) on each cell. S. sanguis I and II both included strains with peritrichous fibrils and tufts of fibrils, but the mixed morphotype strain was confined to biotype II. Fibrils were present on cells at all stages throughout the growth cycle for the strains tested. Freshly isolated fibrillar strains coaggregated consistently well with A. viscosus and A. naeslundii, although some fibrillar reference strains lacked the ability. In addition, all tufted strains could not coaggregate, but the strains with the mixed morphotype coaggregated well. Coaggregation with F. nucleatum was very strong for the fibrillar strains, but less strong for the tufted strains. We discuss the possible correlation between S. sanguis surface structure and ability to coaggregate.

show abstract

Section: Resultsmentioning

confidence: 99%

Surface structures (peritrichous fibrils and tufts of fibrils) found on Streptococcus sanguis strains may be related to their ability to coaggregate with other oral genera

Handley¹,

Carter²,

Wyatt³

et al. 1985

Infect Immun

131

View full text Add to dashboard Cite

show abstract

“…It is known that the immunodeterminant epitope of Lancefield group F, D and H antigens is P-glucosyl-A^acetylgalactosamine (20,37) or glucose residue linked to glycerol phosphate backbone of teichoic acid molecules (6,26,34). However, the chemical composition and immunodeterminant epitope of the type k antigen (Tables 1, 2) was different from those of these antigens.…”

Section: Discussionmentioning

confidence: 99%

“…The cell walls and type antigen preparation of type k and group G strain K214-2K, like those of serotype c strains (16) but unlike those ofthe serotype b, g and i "S. milleri" strains (13,17,39), contained considerable amounts of glycerol and phosphorus (Table 1), suggesting the possible pres-ence of glycerol teichoic acid in this antigen preparation. It should be remembered here that Lancefield group H, D and N antigens are glycerol teichoic acid containing glucose (6,26,34) or galactose (7) as an immunodeter- The molar ratio in parentheses is expressed as glycine.…”

Section: Discussionmentioning

confidence: 99%

Immunochemical characterization of the carbohydrate antigens of serotype k and Lancefield group G “Streptococcus milleri”

Kitada

Inoue

1996

Oral Microbiology and Immunology

View full text Add to dashboard Cite

Carbohydrate antigens of the serotype k/Lancefield group G "Streptococcus milleri" were extracted by autoclaving whole cells of the type k reference strain Streptococcus anginosus K214-2K. The type k and group G antigen molecules are separated from each other and partially purified by a DEAE-Sephadex A25 column chromatography followed by a Sephadex G-100 gel filtration. In the double diffusion and the immunoelectrophoresis, the type k and group G antigen preparations obtained yielded single bands with their homologous antisera respectively. The type k antigen preparation contained principally glycerol, rhamnose, ribitol, galactose and glucose in a molar ratio of 0.54:1.20:0.43:0.33:1.00. The quantitative precipitin inhibition test indicated that beta(1-6)glucosyl-glucose (gentiobiose) sequence played a major role in immunodeterminant structure. Thus, the type k antigen of "S. milleri" appears to be a new carbohydrate type antigen that is chemically different from any Ottens type antigen. In contrast, the group G antigen preparation contained high proportion of rhamnose in addition to glucose, N-acetylglucosamine and N-acetylgalactosamine in a molar ratio of 6.45:1.00:0.24:1.15, and the rhamnose residue was involved in the immunodominant epitope, being in good agreement with the previously proposed chemical structure of the group antigen.

show abstract

“…Interestingly, many S. gordonii and S. sanguis strains in biovars that are positive for LTA (the Lancefield group H antigen [52]) also are positive for galactoside-sensitive adhesins de-tected by intrageneric coaggregation (32,38). The group H antigen occurs in most strains of S. gordonii and S. sanguis and not at all in strains of S. oralis and S. mitis (35).…”

Section: Discussionmentioning

confidence: 99%

Insertional Inactivation of Genes Responsible for the d -Alanylation of Lipoteichoic Acid in Streptococcus gordonii DL1 (Challis) Affects Intrageneric Coaggregations

et al. 1999

View full text Add to dashboard Cite

Most human oral viridans streptococci participate in intrageneric coaggregations, the cell-to-cell adherence among genetically distinct streptococci. Two genes relevant to these intrageneric coaggregations were identified by transposon Tn916 mutagenesis ofStreptococcus gordonii DL1 (Challis). A 626-bp sequence flanking the left end of the transposon was homologous todltA and dltB of Lactobacillus rhamnosus ATCC 7469 (formerly called Lactobacillus casei). A 60-kb probe based on this flanking sequence was used to identify the homologous DNA in a fosmid library of S. gordonii DL1. This DNA encodedd-alanine-d-alanyl carrier protein ligase that was expressed in Escherichia coli from the fosmid clone. The cloned streptococcal dltA was disrupted by inserting anermAM cassette, and then it was linearized and transformed into S. gordonii DL1 for allelic replacement. Erythromycin-resistant transformants containing a single insertion indltA exhibited a loss of d-alanyl esters in lipoteichoic acid (LTA) and a loss of intrageneric coaggregation. This phenotype was correlated with the loss of a 100-kDa surface protein reported previously to be involved in mediating intrageneric coaggregation (C. J. Whittaker, D. L. Clemans, and P. E. Kolenbrander, Infect. Immun. 64:4137–4142, 1996). The mutants retained the parental ability to participate in intergeneric coaggregation with human oral actinomyces, indicating the specificity of the mutation in altering intrageneric coaggregations. The mutants were altered morphologically and exhibited aberrant cell septa in a variety of pleomorphs. The natural DNA transformation frequency was reduced 10-fold in these mutants. Southern analysis of chromosomal DNAs from various streptococcal species with the dltA probe revealed the presence of this gene in most viridans streptococci. Thus, it is hypothesized that d-alanyl LTA may provide binding sites for the putative 100-kDa adhesin and scaffolding for the proper presentation of this adhesin to mediate intrageneric coaggregation.

show abstract

Antigenic determinant of the Lancefield group H antigen of Streptococcus sanguis

Cited by 9 publications

References 37 publications

Surface structures (peritrichous fibrils and tufts of fibrils) found on Streptococcus sanguis strains may be related to their ability to coaggregate with other oral genera

Surface structures (peritrichous fibrils and tufts of fibrils) found on Streptococcus sanguis strains may be related to their ability to coaggregate with other oral genera

Immunochemical characterization of the carbohydrate antigens of serotype k and Lancefield group G “Streptococcus milleri”

Insertional Inactivation of Genes Responsible for the d -Alanylation of Lipoteichoic Acid in Streptococcus gordonii DL1 (Challis) Affects Intrageneric Coaggregations

Contact Info

Product

Resources

About