Lipase Activity among Bacteria Isolated from Amazonian Soils

Abstract

The objective of this study was to select lipase-producing bacteria collected from different counties of the Amazon region. Of the 440 bacteria strains, 181 were selected for the lipase assay in qualitative tests at Petri dishes, being 75 (41%) lipase positive. The enzymatic index was determined during fifteen days at different temperatures (30°, 35°, 40°, and 45°C). The highest lipase activity was observed within 72 hours at 30°C. Twelve bacteria strains presented an index equal to or greater than the standard used like reference, demonstrating the potential of microbial resource. After the bioassay in Petri dishes, the selected bacteria strains were analyzed in quantitative tests on p-nitrophenyl palmitate (p-NPP). A group of the strains was selected for other phases of study with the use in oleaginous substrates of the Amazonian flora, aiming for the application in processes like oil biotransformation.

Hydrolytic activity of bacterial lipases in amazonian vegetable oils

“…The bacterial strains tested for lipase activity were selected after screening by Willerding et al. 20 A set of 181 bacterial isolates was tested in a culture medium as inductor. Of this total, 75 bacterial isolates (41%) showed production of lipase.…”

Recovery of Extracellular Lipolytic Enzymes fromMacrophomina phaseolinaby Foam Fractionation with Air

“…According to the literature [50–52], lipases are carboxylesterases that hydrolyze triacylglycerols, for example, olive oil. Only the sample collected at 50 min of the second experiment (additional Triton used) showed a visible halo indicating a hydrolytic activity over normal chain triglycerides.…”

Production of thermostable multiple enzymes from Bacillus amyloliquefaciens KUB29

“…Contudo, distintos tempos de incubação têm sido reportados para a máxima produção de xilanases: 24 h para Geobacillus stearothermophilus KIBGE-IB29 (Bibi Z et al, 2014), 56 h para Bacillus pumilus VLK-1 (Kumar L et al, 2014), 96 h para Arthrobacter sp.MTCC 6915 (Murugan S et al, 2011) e Bacillus mojavensis AG137 (Sepahy AA, Ghazi S, Sepahy MA, 2011). ótimo para a produção de lipases por bactérias isoladas do solo (Willerding AL et al, 2011;Sahoo RK, Subudhi E, Kumar M, 2014;Koc M, Cokmus C, Cihan AC, 2015).…”