Hydrolytic activity of bacterial lipases in amazonian vegetable oils

Willerding, André Luis; Geraldo, Francisco; Rocha, Mello da; Neto, Carvalho; Areosa, Danilo; Oliveira, Luiz Antônio de

doi:10.1590/s0100-40422012000900015

Cited by 9 publications

(4 citation statements)

References 10 publications

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“…Bussamara et al (2010) also observed a direct relationship between enzymatic activity of lipase and temperature, which increased from 30 to 50°C. The high enzymatic activity at 40°C of lipases isolated from aqueous waste bacteria was similar to that obtained by lipases from Gram negative bacteria belonging to genus Pseudomonas studied by Gaur, Gupta and Khare (2008) and also bacteria of genus Klebsiella variicola studied by Willerding at al (2012). STP (Grease trap from the Sewage Treatment Plant), T (treatment tank) and R (residential grease trap), S1 (culture medium: pure effluent) and S2 (culture medium: minimal mineral medium + soybean oil).…”

Section: Lipolytic Bacteriasupporting

confidence: 71%

Production of lipase extrated from aqueous waste: enzymatic activity kinetics

2014

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Lipases are an important group of enzymes with various applications in the food, chemical and pharmaceutical industry, besides having great interest for the treatment of effluents with high lipid content. The objective of this study was to isolate, characterize and select lipolytic bacteria that produce lipase from aqueous waste effluents and to study the enzymatic activity kinetics of the extract obtained via submerged fermentation. The results obtained are promising, being possible to isolate and characterize 23 lipase-producing microorganisms, mostly gram-positive bacteria, but after the fermentation step in a liquid medium, gram negative bacteria showed the highest enzymatic activity (56.72 U.L -1 for STP 2A` bacterium and 81.99 U.L -1 for R2B). In the enzymatic activity kinetic study with the selected bacterium (R2B), among the six variables (temperature, pH, minimal mineral medium, soybean oil, glucose and sodium nitrate), temperature was the one that most positively influenced the enzymatic activity, and the best results were obtained at 40°C. It was concluded that the enzyme extract obtained from environmental waste may be used to treat the effluent and contribute to reduce environmental impacts.Index terms: Microbial extract, effluent treatment, sustainability. RESUMOAs lipases são um importante grupo de enzimas com diversas aplicações na indústria alimentar, química e farmacêutica, além de ter grande interesse para o tratamento de efluentes com alto teor de lipídios. Objetivou-se, neste trabalho, isolar, caracterizar e selecionar bactérias lipolíticas produtoras de lipase, a partir de efluentes residuais aquosos, assim como estudar a cinética da atividade enzimática do extrato obtido via fermentação submersa. Os resultados obtidos foram promissores, sendo possível isolar e caracterizar 23 microrganismos potencialmente produtores de lipase, sendo a maioria das bactérias Gram Positivas, porém após a etapa de fermentação em meio líquido, os Gram Negativos apresentaram as maiores atividades enzimáticas (56,72 U.L -1 para a bactéria ETE 2A` e 81,99 U.L -1 para a R2B). No estudo cinético da atividade enzimática com a bactéria selecionada (R2B), das seis variáveis (temperatura, pH, meio mínimo mineral, óleo de soja, glicose, e nitrato de sódio), a temperatura foi a que mais influenciou na atividade enzimática, tendo efeito positivo, e os melhores resultados foram obtidos a 40 °C. Concluiu-se que o extrato enzimático obtido, a partir de resíduos ambientais, poderão ser utilizados no próprio tratamento do efluente e contribuir na redução dos impactos ambientais.Termos para indexação: Extrato microbiano, tratamento de efluentes, sustentabilidade.

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Section: Lipolytic Bacteriasupporting

confidence: 71%

Production of lipase extrated from aqueous waste: enzymatic activity kinetics

2014

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“…The enzyme stability toward pH was prepared according to Babaki et al and Willerding et al [18,19], where 5 mg of each enzyme was incubated in 4 mL of 25 mM phosphate buffer in 40 °C for 5 minutes. The pH of phosphate buffer was varied from 6 to 10.…”

Section: Methodsmentioning

confidence: 99%

Activity and Stability of Immobilized Lipase for Utilization in Transesterification of Waste Cooking Oil

Gusniah

Veny

Hamzah

2020

Bull. Chem. React. Eng. Catal.

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Biodiesel is fatty acid methyl ester that commonly derived from vegetable oils and animal fats that can be produced through enzymatic transesterification using lipase. In this study, three different types of lipase were used, which are Lipase Immobilized Pseudomonas cepacia, PcL, Thermomyces lanuginosus, TLIM, and Candida Antarctica A (recombinant from Aspergillus oryzae), CALA. These lipases were compared based on their activity at different pH (6-10), temperature (30-50 °C), activation energy, and amount of lipase loading for hydrolysis of p-NPA into n-NP. The result indicates that among the lipase used in the study, CALA is the preferable biocatalyst in the hydrolysis of p-NPA due to the minimum energy required and higher enzymatic activity at 20 mg of enzyme loading. PcL and CALA used in the study gave the optimum activity at pH 9 except for TLIM at pH 8 and the optimum temperature at 40 °C. The kinetic data obtained for CALA in this reaction were Km = 57.412 mM and Vm = 70 µM/min. This finding shows that CALA is beneficial biocatalysts for the transesterification process to obtain a higher product with lower activation energy. Copyright © 2020 BCREC Group. All rights reserved

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“…In the Amazonian region, there are countless species whose products can contribute to meet the demand for seeds, fruit, nuts, and their oleaginous oils. Vegetable oils from the Amazon are used by some industries but remain small exploited commercially (4). However, the demand for vegetable oils with unique composition is increasing, and oils obtained from native palm fruits, which are complex mixtures of organic compounds (5), can be considered as new sources that will surely have their increased commercial value (6).…”

Section: Introductionmentioning

confidence: 99%

“…From the fruit pulp, the obtained oils are rich in oleic acids; while seeds are rich in lauric acid. The pulp oil has a high content of carotene in nature (pro-Vitamin A), and this characteristic makes this oil useful for the manufacture of sunscreen (4,9). Tucumã (Astrocaryum vulgare) is a palm with an orange-yellow fruit (about the size of a chicken egg), which is an excellent source of carotenoids.…”

Section: Introductionmentioning

confidence: 99%