LINT‐Web: A Web‐Based Lipidomic Data Mining Tool Using Intra‐Omic Integrative Correlation Strategy

Li, Fengsheng; Song, Jia; Zhang, Yingkun; Wang, Shuaikang; Wang, Jinhui; Lin, Li; Yang, Chaoyong; Huang, He

doi:10.1002/smtd.202100206

Cited by 13 publications

(13 citation statements)

References 53 publications

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“…MS-based lipidomics has been widely employed in cancer research aimed at finding new biomarkers and mechanisms of drug action. 38 In this technique, biological samples were crushed and homogenized to extract lipids, which lead to the loss of information of spatial distribution of lipids in biological samples. This information might play an important role in clarifying the mechanisms of drug action.…”

Section: Resultsmentioning

confidence: 99%

Three-Dimensional Mass Spectrometry Imaging Reveals Distributions of Lipids and the Drug Metabolite Associated with the Enhanced Growth of Colon Cancer Cell Spheroids Treated with Triclosan

Xie

Zhang

et al. 2022

Anal. Chem.

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The application of mass spectrometry imaging (MSI) to explore the responses of cancer cell spheroids (CCS) after treatment of exogenous molecules has attracted growing attention. Increasing studies have utilized MSI to image the two-dimensional distributions of exogenous and endogenous molecules in planar CCS sections. However, because CCS are volumetric and heterogenous, maintaining their three-dimensional (3D) information is essential for acquiring a better understanding of the tumor microenvironment and mechanisms of action of exogenous molecules. Here, an established method of 3D MSI was applied to distinguish the distributions of triclosan sulfate and endogenous lipids in three microregions of colon CCS with an enhanced growth induced by the treatment of triclosan, a common antimicrobial agent. The results of 3D MSI showed that triclosan sulfate gradually accumulated from the periphery to the entire structure of CCS and finally localized in the core region. Spatial lipidomics analysis revealed that the upregulated phosphatidylethanolamine (fold change (FD) = 1.26, p = 0.0021), phosphatidylinositol (FD = 1.17, p = 0.0180), and phosphatidylcholine (FD = 1.22, p = 0.0178) species mainly distributed in the outer proliferative region, while the upregulated sphingomyelin (FD = 1.18, p = 0.024) species tended to distribute in the inner necrotic region. Our results suggest that a competitive mechanism between inhibiting and promoting CCS growth might be responsible for the proliferation of CCS treated with triclosan.

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Section: Resultsmentioning

confidence: 99%

Three-Dimensional Mass Spectrometry Imaging Reveals Distributions of Lipids and the Drug Metabolite Associated with the Enhanced Growth of Colon Cancer Cell Spheroids Treated with Triclosan

Xie

Zhang

et al. 2022

Anal. Chem.

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“…Then, the relative abundance of individual metabolites/lipids and the mean abundance of ceramides was calculated based on the processed data. The details of how these tools work were described in the literature (Pang et al , 2020 ) and (Li et al , 2021a ). The raw data were deposited at Metabolights as MTBLS6249 (lipid) and MTBLS6250 (metabolites).…”

Section: Methodsmentioning

confidence: 99%

BCAA insufficiency leads to premature ovarian insufficiency via ceramide‐induced elevation of ROS

Guo

Zhu

et al. 2023

EMBO Mol Med

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Premature ovarian insufficiency (POI) is a disease featured by early menopause before 40 years of age, accompanied by an elevation of follicle‐stimulating hormone. Though POI affects many aspects of women's health, its major causes remain unknown. Many clinical studies have shown that POI patients are generally underweight, indicating a potential correlation between POI and metabolic disorders. To understand the pathogenesis of POI, we performed metabolomics analysis on serum and identified branch‐chain amino acid (BCAA) insufficiency‐related metabolic disorders in two independent cohorts from two clinics. A low BCAA diet phenotypically reproduced the metabolic, endocrine, ovarian, and reproductive changes of POI in young C57BL/6J mice. A mechanism study revealed that the BCAA insufficiency‐induced POI is associated with abnormal activation of the ceramide‐reactive oxygen species (ROS) axis and consequent impairment of ovarian granulosa cell function. Significantly, the dietary supplement of BCAA prevented the development of ROS‐induced POI in female mice. The results of this pathogenic study will lead to the development of specific therapies for POI.

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“…Then, the relative abundance was calculated based on the processed data. The details of how these tools work are described in the literature [ 15 , 16 ].…”

Section: Methodsmentioning

confidence: 99%

The Storage Conditions of High-Fat Diet Are the Key Factors for Diet-Induced Obesity and Liver Damage

Guo

et al. 2022

Nutrients

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The diet-induced obesity (DIO) mouse model has been widely used for obesity studies. The effects of storage conditions on the composition of nutrients in high-fat diets (HFDs) and their impact on metabolic homeostasis have not been systemically investigated. In the current study, we tested the effects of HFDs stored under different conditions and found that mice fed a HFD stored in the fridge (HFDfri) gained less weight than those fed HFDs stored in the freezer (HFDfre). Further analysis revealed that changes in the relative abundance of medium-chain triglyceride (MCT) in the HFDfri, which have much lower intestinal absorption rates, contributed to the body weight differences. In contrast, exacerbated liver damage and elevated levels of unfolded protein response (UPR) was observed in the mice fed by HFDfri. Depletion of the UPR-regulated gene Nnmt alleviated liver damage via the inhibition of the integrated stress response (ISR). Our study, for the first time, provides evidence that HFD storage conditions can have a significant impact on both body weight changes and liver damage in the DIO model.

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LINT‐Web: A Web‐Based Lipidomic Data Mining Tool Using Intra‐Omic Integrative Correlation Strategy

Cited by 13 publications

References 53 publications

Three-Dimensional Mass Spectrometry Imaging Reveals Distributions of Lipids and the Drug Metabolite Associated with the Enhanced Growth of Colon Cancer Cell Spheroids Treated with Triclosan

Three-Dimensional Mass Spectrometry Imaging Reveals Distributions of Lipids and the Drug Metabolite Associated with the Enhanced Growth of Colon Cancer Cell Spheroids Treated with Triclosan

BCAA insufficiency leads to premature ovarian insufficiency via ceramide‐induced elevation of ROS

The Storage Conditions of High-Fat Diet Are the Key Factors for Diet-Induced Obesity and Liver Damage

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