Linking the Solution Viscosity of an IgG2 Monoclonal Antibody to Its Structure as a Function of pH and Temperature

Temperature-Ramped Studies on the Aggregation, Unfolding, and Interaction of a Therapeutic Monoclonal Antibody

“…However, in the context of the presented conversion with respect to the relatively small value of the partial specific volume of about 0.730–0.739 mL/g for MAbs, the difference is assumed to be of minor influence on the overall fit. Moreover, Cheng et al just recently investigated the partial specific volume of an immunoglobulin G2 at two different pH values. They found similar values for a citrate–phosphate formulation buffer at pH 7.0 and 4.0.…”

Viscosity measurement based on the tapping-induced free vibration of sessile droplets using MEMS-based piezoresistive cantilevers

“…Moreover, viscosity is also an important indicator for the characterisation of antibody solutions and protein-based drugs [3][4][5][6][7][8][9][10][11][12][13] as well as for DNA analysis 14,15 . For example, measuring the viscosity of blood plasma is important in blood coagulation tests 1, 2 .…”

A Microliter Capillary Rheometer for Characterization of Protein Solutions

“…[4][5][6][7][8][9] Although there is not a clearly established relationship between viscosity and protein selfassociation and/or aggregation, considerable work is aimed at this goal. 10 Despite the maturity of viscometry, there remains a need for a rheometer that meets the following three needs of the biopharamaceutical industry: small volume; large dynamic range of shear rates; and no air-sample interface. The small volume requirement is particularly important to the biopharmaceutical industry during the screening stage of product develop-Correspondence to: Steven D. Hudson (Telephone: +301-975-6579; Fax: +301-975-4924; E-mail: steven.hudson@nist.gov) This article contains supplementary material available from the authors upon request or via the Internet at http://wileylibrary.com.…”