Linking Enzymatic Oxidative Degradation of Lignin to Organics Detoxification

Wang, Xiaolu; Yao, Bin; Su, Xiaoyun

doi:10.3390/ijms19113373

Cited by 87 publications

(54 citation statements)

References 137 publications

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“…Although DyP was reported to be involved in lignin degradation, industrial dyes decolorization [ 21 , 28 ], the great application value of DyP in xenobiotic bioremediation gradually attracted more and more attentions in recent years [ 29 , 30 ]. In this study, the different types of mycotoxins including AFB 1 , ZEN and DON were used to evaluate the mycotoxin degrading capability of B sDyP from B. subtilis SCK6.…”

Section: Resultsmentioning

confidence: 99%

Enzymatic Degradation of Multiple Major Mycotoxins by Dye-Decolorizing Peroxidase from Bacillus subtilis

Qin

et al. 2021

Toxins

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The co-occurrence of multiple mycotoxins, including aflatoxin B1 (AFB1), zearalenone (ZEN) and deoxynivalenol (DON), widely exists in cereal-based animal feed and food. At present, most reported mycotoxins degrading enzymes target only a certain type of mycotoxins. Therefore, it is of great significance for mining enzymes involved in the simultaneous degradation of different types of mycotoxins. In this study, a dye-decolorizing peroxidase-encoding gene BsDyP from Bacillus subtilis SCK6 was cloned and expressed in Escherichia coli BL21/pG-Tf2. The purified recombinant BsDyP was capable of oxidizing various substrates, including lignin phenolic model compounds 2,6-dimethylphenol and guaiacol, the substrate 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), anthraquinone dye reactive blue 19 and azo dye reactive black 5, as well as Mn2+. In addition, BsDyP could efficiently degrade different types of mycotoxins, including AFB1, ZEN and DON, in presence of Mn2+. More important, the toxicities of their corresponding enzymatic degradation products AFB1-diol, 15-OH-ZEN and C15H18O8 were significantly lower than AFB1, ZEN and DON. In summary, these results proved that BsDyP was a promising candidate for the simultaneous degradation of multiple mycotoxins in animal feed and food.

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Section: Resultsmentioning

confidence: 99%

Enzymatic Degradation of Multiple Major Mycotoxins by Dye-Decolorizing Peroxidase from Bacillus subtilis

Qin

et al. 2021

Toxins

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“…Their potential for the biodegradation of organic pollutants lies in several major oxidative enzyme activities, mainly laccases (benzenediol:oxygen oxidoreductases, Lac, EC 1.10.3.2) and peroxidases, including manganese peroxidases (MnP, EC 1.11.1.13), lignin peroxidases (LiP, EC 1.11.1.14), and versatile peroxidases (VP, EC 1.11.1.16), among others [ 1 ]. These enzymes have been employed in the degradation of a wide range of pollutants, such as endocrine disrupting chemicals [ 2 ], dyes [ 3 ], pesticides [ 4 ], substituted phenols, pharmaceuticals [ 5 ], and others. Significant research efforts have been devoted to the degradation of olive oil mill wastewater (OOMW), the liquid byproduct of three-phase decanters of olive oil production plants.…”

Section: Introductionmentioning

confidence: 99%

Crosslinked Enzyme Aggregates (CLEAs) of Laccases from Pleurotus citrinopileatus Induced in Olive Oil Mill Wastewater (OOMW)

Zerva

Pentari

2020

Molecules

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The enzymatic factory of ligninolytic fungi has proven to be a powerful tool in applications regarding the degradation of various types of pollutants. The degradative potential of fungi is mainly due to the production of different types of oxidases, of which laccases is one of the most prominent enzymatic activities. In the present work, crude laccases from the supernatant of Pleurotus citrinopileatus cultures grown in olive oil mill wastewater (OOMW) were immobilized in crosslinked enzyme aggregates (CLEAs), aiming at the development of biocatalysts suitable for the enzymatic treatment of OOMW. The preparation of laccase CLEAs was optimized, resulting in a maximum of 72% residual activity. The resulting CLEAs were shown to be more stable in the presence of solvents and at elevated temperatures compared to the soluble laccase preparation. The removal of the phenolic component of OOMW catalyzed by laccase-CLEAs exceeded 35%, while they were found to retain their activity for at least three cycles of repetitive use. The described CLEAs can be applied for the pretreatment of OOMW, prior to its use for valorization processes, and thus, facilitate its complete biodegradation towards a consolidated process in the context of circular economy.

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“…Many technologies have been applied to enhance activity and increase catalytic characteristics of LiPs, such as LiP entrapment in calcium beads [37]. 4) [32,36].…”

Section: Peroxidasesmentioning

confidence: 99%

Recent Advances in Enzymes for the Bioremediation of Pollutants

Mousavi

Hashemi

Moezzi

et al. 2021

Biochemistry Research International

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Nowadays, pollution of the environment is a huge problem for humans and other organisms’ health. Conventional methods of pollutant removal like membrane filtration or ion exchange are not efficient enough to lower the number of pollutants to standard levels. Biological methods, because of their higher efficiency and biocompatibility, are preferred for the remediation of pollutants. These cost-effective and environment-friendly methods of reducing pollutants are called bioremediation. In bioremediation methods, enzymes play the most crucial role. Enzymes can remedy different types of organic and inorganic pollutants, including PAHs, azo dyes, polymers, organocyanides, lead, chromium, and mercury. Different enzymes isolated from various species have been used for the bioremediation of pollutants. Discovering new enzymes and new subtypes with specific physicochemical characteristics would be a promising way to find more efficient and cost-effective tools for the remediation of pollutants.

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Linking Enzymatic Oxidative Degradation of Lignin to Organics Detoxification

Cited by 87 publications

References 137 publications

Enzymatic Degradation of Multiple Major Mycotoxins by Dye-Decolorizing Peroxidase from Bacillus subtilis

Enzymatic Degradation of Multiple Major Mycotoxins by Dye-Decolorizing Peroxidase from Bacillus subtilis

Crosslinked Enzyme Aggregates (CLEAs) of Laccases from Pleurotus citrinopileatus Induced in Olive Oil Mill Wastewater (OOMW)

Recent Advances in Enzymes for the Bioremediation of Pollutants

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