Linkage mapping of sbm-1, a gene conferring resistance to pea seed-borne mosaic virus, using molecular markers in Pisum sativum

Timmerman, G. M.; Frew, T. J.; Miller, Allison L.; Weeden, N. F.; Jermyn, W. A.

doi:10.1007/bf00220920

Cited by 59 publications

(30 citation statements)

References 24 publications

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“…Resistances to all three pathotypes of PSbMV in P. sativum are inherited as single recessive genes, designated sbm-1, sbm-2, sbm-3, and sbm-4 (26,27). The sbm genes were mapped to linkage groups II (sbm-2) and VI (sbm-1, sbm-3, and sbm-4) as part of two clusters of recessive resistance genes affecting several potyviruses (29,38). Resistance gene sbm-2 was mapped in category C P. sativum cultivar Bonneville (26), which is resistant to P-2 and susceptible to both P-1 and P-4 (1).…”

mentioning

confidence: 99%

Recessive Resistance in Pisum sativum and Potyvirus Pathotype Resolved in a Gene-for-Cistron Correspondence between Host and Virus

Johansen¹,

Lund²,

Hjulsager³

et al. 2001

J Virol

102

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Pea seed-borne mosaic potyvirus (PSbMV) isolates are divided into pathotypes P-1, P-2, and P-4 according to their infection profile on a panel of Pisum sativum lines. P. sativum PI 269818 is resistant to P-1 and P-2 isolates and is susceptible to P-4 isolates. Resistance to P-1 is inherited as a single recessive gene, denoted sbm-1, and the pathogenicity determinant has previously been mapped to the virus-coded protein VPg. In the cultivar Bonneville, a second recessive gene, sbm-2, confers specific resistance to P-2. By exchanging cistrons between a P-2 and a P-4 isolate, the P3-6k1 cistron was identified as the PSbMV host-specific pathogenicity determinant on Bonneville. Exchange of P3-6k1 did not affect infection on PI 269818, and infection of Bonneville was not altered by substitution of the VPg cistron, indicating that P3-6k1 and VPg are independent determinants of pathotype-specific infectivity. On PI 269818 the pathogenicity determinant of both P-1 and P-2 mapped to the N terminus of VPg. This suggests that VPg from the P-1 and P-2 isolates are functionally similar on this host and that resistance to P-1 and P-2 in PI 269818 may operate by the same mechanism. Identification of VPg-sbm-1 and P3-6k1-sbm-2 as independent pairs of genetic interactors between PSbMV and P. sativum provides a simple explanation of the three known pathotypes of PSbMV. Furthermore, analysis of ␤-glucuronidase-tagged P-2 virus indicated that sbm-2 resistance affected an early step in infection, implying that the P3-6k1 region plays a critical role in potyvirus replication or cell-to-cell movement.

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mentioning

confidence: 99%

Recessive Resistance in Pisum sativum and Potyvirus Pathotype Resolved in a Gene-for-Cistron Correspondence between Host and Virus

Johansen¹,

Lund²,

Hjulsager³

et al. 2001

J Virol

102

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“…The previous closest markers to sbm-1 were GS185 (Timmerman et al 1993) and sG05_2537 (Frew et al 2002) at 8 cM and~4 cM, respectively, and our cDNA-AFLP search yielded only one closer marker, ZG10, at 0.7 cM. Alignment of our data and the published (Frew et al 2002) maps shows the relative positions of ZG10, eIF4E/sbm-1 and G05_2537 (Fig.…”

Section: Discussionmentioning

confidence: 54%

Identification of markers tightly linked to sbm recessive genes for resistance to Pea seed-borne mosaic virus

et al. 2004

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Two virus resistance loci on linkage groups II and VI have provided the only sources of natural resistance against Pea seed-borne mosaic virus (PSbMV, Potyviridae) in the important crop plant Pisum sativum L. A combination of parallel approaches was used to collate linked markers, particularly for sbm-1 resistance on linkage group VI. We have identified sequences derived from the genes for the eukaryotic translation initiation factors eIF4E and eIF(iso)4E as being very tightly linked to the resistance gene clusters on linkage groups VI and II, respectively. In particular, no recombinants between sbm-1 and eIF4E were found amongst 500 individuals of an F2 cross between the BC4 resistant line (JI1405) and its recurrent susceptible parent 'Scout'. In a different mapping population, the gene eIF(iso)4E was also shown to be linked to sbm-2 on linkage group II. A parallel cDNA-AFLP comparison of pairs of resistant and susceptible lines also identified an expressed tag marker just 0.7 cM from sbm-1. eIF4E and eIF(iso)4E have been associated with resistance to related viruses in other hosts. This correlation strengthens the use of our markers as valuable tools to assist in breeding multiple virus resistances into peas, and identifies potential targets for resistance gene identification in pea.

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“…However, neither of these morphological markers were particularly close, nor were they desired in the final cultivar . Many molecular markers have been mapped to this chromosome, and the linkage relationships of some of these markers were examined in a series of segregating populations Weeden et al ., 1991 ;Timmerman et al ., 1993) . The closest marker to sbm-1 appears to be the gene coding the plastid-specific glutamine synthetase, Gsp .…”

Section: The Tagging Of Disease Resistance Genes In Peamentioning

confidence: 99%

“…The potential value of Gs-p as a marker was further demonstrated when the resistance/susceptible phenotypes of 32 pea lines were compared with the hybridization pattern generated by GS 185 . Of the resistant lines examined, most (12 out of 15) displayed a GS 185 hybridization pattern which was not observed among the 17 susceptible lines tested (Timmerman et al ., 1993) . Most of the resistant lines also possessed the PRX-3 allozyme with the slower mobility, although the distinction between the resistant and susceptible groups was not as marked as for the GS 185 pattern.…”

Section: The Tagging Of Disease Resistance Genes In Peamentioning

confidence: 99%

Identifying and mapping genes of economic significance

Weeden

Timmerman²,

Lü

1993

Euphytica

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An understanding of the genetic basis of characters of commercial importance is critical if a breeder is attempting to move such characters into breeding material . A number of particularly interesting characters or genes have been identified in cool season food legumes, and in pea many of these have been "tagged" by molecular markers such as allozyme or DNA polymorphisms . This process of mapping and tagging genes has been greatly accelerated by recent developments in molecular biology. It appears that markers will soon be available for many genes in lentil, faba bean, and chickpea and that genetic knowledge developed in one crop will have significant applications in the other cool season food legumes .

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Linkage mapping of sbm-1, a gene conferring resistance to pea seed-borne mosaic virus, using molecular markers in Pisum sativum

Cited by 59 publications

References 24 publications

Recessive Resistance in Pisum sativum and Potyvirus Pathotype Resolved in a Gene-for-Cistron Correspondence between Host and Virus

Recessive Resistance in Pisum sativum and Potyvirus Pathotype Resolved in a Gene-for-Cistron Correspondence between Host and Virus

Identification of markers tightly linked to sbm recessive genes for resistance to Pea seed-borne mosaic virus

Identifying and mapping genes of economic significance

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