Identification of markers tightly linked to sbm recessive genes for resistance to Pea seed-borne mosaic virus

Abstract

Two virus resistance loci on linkage groups II and VI have provided the only sources of natural resistance against Pea seed-borne mosaic virus (PSbMV, Potyviridae) in the important crop plant Pisum sativum L. A combination of parallel approaches was used to collate linked markers, particularly for sbm-1 resistance on linkage group VI. We have identified sequences derived from the genes for the eukaryotic translation initiation factors eIF4E and eIF(iso)4E as being very tightly linked to the resistance gene clusters on linkage groups VI and II, respectively. In particular, no recombinants between sbm-1 and eIF4E were found amongst 500 individuals of an F2 cross between the BC4 resistant line (JI1405) and its recurrent susceptible parent 'Scout'. In a different mapping population, the gene eIF(iso)4E was also shown to be linked to sbm-2 on linkage group II. A parallel cDNA-AFLP comparison of pairs of resistant and susceptible lines also identified an expressed tag marker just 0.7 cM from sbm-1. eIF4E and eIF(iso)4E have been associated with resistance to related viruses in other hosts. This correlation strengthens the use of our markers as valuable tools to assist in breeding multiple virus resistances into peas, and identifies potential targets for resistance gene identification in pea.

Mapping and candidate-gene screening of the novel Turnip mosaic virus resistance gene retr02 in Chinese cabbage (Brassica rapa L.)

“…Gómez, et al (2009) mainly attributed to the absence of susceptibility factors, such as eIF4E, eIF(iso)4E, eIF4G, eIF(iso)4G, e.g. lsp1 (Lellis et al 2002), pvr2 (Ayme et al 2006), nsv (Nieto et al 2006;Truniger et al 2008), cum2-1 (Yoshii et al 1998), cum1-1 (Yoshii et al 1998, sbm1 (Gao et al 2004), rymv-1 (Albar et al 2003;Albar et al 2006), rym4/5 (Stein et al 2005). A number of recessive resistances to other members of the Potyviridae correspond to eIF4E or eIF(iso)4E (Robaglia and Caranta 2006).…”

Three Sequenced Legume Genomes and Many Crop Species: Rich Opportunities for Translational Genomics

“…The garden pea is in some senses the founding model for genetics, through Mendel's work in 1866. Pea has been further developed as a model for development traits such as compound leaf form (Tattersall et al, 2005), tendril formation (Hofer et al, 2009), transposon evolution (Jing et al, 2005), defense responses (Gao et al, 2004), starch and sugar synthesis (Barratt et al, 2001), and hormonal control of shoot branching (Beveridge et al, 2009). Common bean has been used to characterize the molecular basis of photoperiod sensitivity and determinacy (Kwak et al, 2008).…”

Quantitative and Qualitative Involvement of P3N-PIPO in Overcoming Recessive Resistance against Clover Yellow Vein Virus in Pea Carrying the cyv1 Gene

“…The eIF(iso)4E gene is also found in LG II (28). However, the recessive genes (i.e., cyv1, mo, and sbm-2) are not likely to be eIF(iso)4E because there was no difference in the deduced amino acid sequences of the eIF(iso)4E proteins between susceptible and resistant pea lines (26,28). Thus, if virus isolates emerge that overcome these recessive resistance genes, the viral determinant responsible might not be VPg.…”

“…The recessive resistance genes cyv1 (resistance to ClYVV), mo (resistance to BYMV), and sbm-2 (resistance to PSbMV) were mapped close to one another in pea linkage group (LG) II (26,27). The eIF(iso)4E gene is also found in LG II (28). However, the recessive genes (i.e., cyv1, mo, and sbm-2) are not likely to be eIF(iso)4E because there was no difference in the deduced amino acid sequences of the eIF(iso)4E proteins between susceptible and resistant pea lines (26,28).…”