LINC00346 promotes hepatocellular carcinoma progression via activating the JAK‐STAT3 signaling pathway

Abstract

Hepatocellular carcinoma (HCC) remains the most common malignant tumor worldwide. Long noncoding RNAs can modulate various tumorigenic processes. In addition, growing evidence has indicated tha the Janus kinase–signal transducer and activator of transcription (JAK‐STAT) pathway is activated in multiple cancers, including HCC. Recently, it was found that LINC00346 can participate in several cancers. Nevertheless, the biological roles of LINC00346 in HCC have been barely investigated. In this study, the function of LINC00346 was specifically concentrated upon. We observed that LINC00346 was obviously elevated in HCC cells (Bel7404, Huh‐6, HepG2, and QGY‐7703 cells). Then, Bel7404 and HepG2 cells were overexpressed with LINC00346. Overexpression of LINC00346 repressed HCC cell survival and cell proliferation. In addition, apoptosis of Bel7404 and HepG2 cells was triggered by LINC00346 upregulation. Bel7404 and HepG2 cell cycle was arrested in the G1 phase by LINC00346. Meanwhile, we conducted wound‐healing assay and Transwell invasion assays. As shown, we observed that the migratory and invasive capacities of Bel7404 and HepG2 cells were remarkably restrained by the increase of LINC00346. Moreover, we showed that LINC00346 overexpression activated the JAK‐STAT3 pathway, which is involved in many cancers. Afterward, in vivo experiments were utilized and we proved that LINC00346 was able to induce HCC tumor growth via activating the JAK‐STAT3 pathway. To conclude, we revealed the potential possibility of developing LINC00346 as an indicator for HCC.

Exosomes function as nanoparticles to transfer miR-199a-3p to reverse chemoresistance to cisplatin in hepatocellular carcinoma

“…Hepatocellular carcinoma (HCC), which is one of the most common malignancies all over the world, is a major cause of death associated with cancer [ 1 ]. The global death of HCC has been recorded 700,000 annually [ 2 ].…”

Long noncoding RNA LINC00346 promotes glioma cell migration, invasion and proliferation by up‐regulating ROCK1

“…Glioma is one of the most common malignant brain tumours; therefore, investigation of the molecular mechanisms involved in glioma progression is essential for the effective treatment of this cancer 32 . LINC00346 levels are increased in hepatocellular carcinoma, pancreatic cancer and gastric cancer, thus contributing to cancer progression 14,15,33 . In the present study, GEPIA data analysis revealed that LINC00346 was up‐regulated in GBM tissue samples and was associated with a poor prognosis, suggesting that LINC00346 may be involved in glioma progression.…”

“…Xu et al showed that LINC00346 gene expression levels are increased in gastric cancer, and its expression level positively correlates with a more advanced pathologic stage and poor prognosis 14 . Yin et al observed that LINC00346 expression levels are elevated in hepatocellular carcinoma cells, thereby promoting hepatocellular carcinoma progression 15 . Nonetheless, the function of LINC00346 in glioma progression remains unclear.…”

Comprehensive analysis of partial epithelial mesenchymal transition‐related genes in hepatocellular carcinoma

“…For example, SNHG3 promotes EMT and sorafenib resistance via activating miR‐128/CD151 axis in HCC 44 . LINC00346 promotes HCC development via JAK‐STAT3 signalling pathway activation 45 . Overexpression of SNHG20 has been reported to be an indicator of poor prognosis of HCC 46 .…”