Lignocellulolytic activities of a novel strain of <i>Trichoderma harzianum</i>

Abstract

This study describes a novel dark-green spore producing strain of Trichoderma harzianum exhibiting higher activities of cellulase, hemicellulase and ligninase on specific plate assays. To assess the cellulase production in detail, basal salt medium (BSM) was supplemented with synthetic [carboxymethyl cellulose (CMC), glucose, sucrose, dextrose, lactose or maltose] and natural (flours of banana, banana peel, jack seed, potato or tapioca) carbon as well as nitrogen (yeast extract, beef extract, peptone, NaNO 3 or NH 4 NO 3) sources. Temperature and pH optima were 28˚C and 4, respectively for the growth of the fungus in CMC-BSM with 146 U/ml cellulase activity. Flours of potato and banana supported comparable yields of cellulase to that of CMC (147 U/ml and 168 U/ml, respectively), while sodium nitrate was the preferred nitrogen source (150 U/ml). The water soluble yellowish-green pigment (a probable siderophore) extracted from the spores showed an absorption maximum at 414 nm. To comprise, this fungus shows the complete lignocellulolytic potential which offers great industrial significance, especially for the ethanol production from the lignocellulosic waste coupled with the production of a new pigment.

Process Optimization for Endoglucanase Production by in Submerged Fermentation

“…Maximum enzyme production was achieved with NaNO 3 as best nitrogen source. Similar findings were also reported by Rubeena et al [14]. Akinola et al [11] reported casein as a best nitrogen source for endoglucanase production by T. harzianum in submerged fermentation.…”

Quality of Agricultural by Products Fermented by Trichoderma harzianum

“…Table 2 shows that T. harzianum is more effective to increase crude protein content and decrease crude fibre in substrat contained high fibre such as pineapple peel than that of low fibre such as banana peel. It is in agreement with Rubeena et al, (2013) who stated that T. harzianum was potential to hydrolize biomass due to its elevated cellulose production and cellulolytic activity. DelgadoJarana et al, (2002) stated that T. harzianum has protease activity and was not responsible on glucanase degradation.…”

Evaluation of Antimicrobial Activity and Identification of Yellow Pigmented Marine Sponge-Associated Fungi from Teluk Awur, Jepara, Central Java

“…Fungus MT23 produced yellow pigment started at the fourth day of cultivation and ready to be harvested on day fourteenth. Pradeep and Pradeep (2013) studied the optimum cultivation condition for pigment production of fungus Fusarium moniliforme. The fungus had highest pigment absorbance (AU 500nm) at temperature 28-30 o C, pH 5.5, glucose as carbon source, peptone as nitrogen source and cultivated for 8 days.…”

“…Environmental conditions have important role in fungi growth. Pradeep and Pradeep (2013) studied the optimum cultivation condition for growth of fungus Fusarium moniliforme. The fungus had highest biomass (g/L) at temperature 28 o C, pH 5.5, glucose as carbon source, peptone as nitrogen source, KH 2 PO 4 as source of salt and addition of methionine as additional amino acid.…”

“…As a result, pigment production in malt extract was higher than other medium. In addition, nutrient content in media can regulate the expression of genes to activate the metabolic path way for pigments production (Pradeep et al, 2013).…”