Lid and Conjunctival Micro Biota During Contact Lens Wear in Children

Sankaridurg, Padmaja; Markoulli, Maria; Jara, Percy Lazon de la; Harmis, Najat; Varghese, Thomas; Willcox, Mark; Holden, Brien A.

doi:10.1097/opx.0b013e318199d20c

Cited by 39 publications

(24 citation statements)

References 11 publications

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“…and C-NS to favour C-NS. CL wear serves to decrease the aerobic conjunctival microbiome population, 37 which might explain the higher proportion of sterile ('no aerobic') counts noted in the CL wearers (Fig. 2).…”

Section: Discussionmentioning

confidence: 99%

Ocular surface microbiome in meibomian gland dysfunction

Watters

Turnbull

Swift

et al. 2016

Clinical Exper Ophthalmology

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Section: Discussionmentioning

confidence: 99%

Ocular surface microbiome in meibomian gland dysfunction

Watters

Turnbull

Swift

et al. 2016

Clinical Exper Ophthalmology

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“…Sankaridurg et al employed longitudinal monitoring of microbial presence at the conjunctiva-lid margin area and reported that in contact lens-wearing children age 8–14, microbial growth was detected in 36% of the conjunctival swabs and in 54% of the lid swabs. 28 The microbial types detected were CNS , Propionibacterium sp , Bacillus sp , Streptococcus sp , Micrococcus sp , S. aureus, and Corynebacterium sp (in the order of frequency). There were no differences in the microbial types recovered from non-contact lens wearers and wearers of HEMA-based soft lenses for a period of 2 years.…”

Section: What Is the Impact Of Contact Lens Wear On Ocular Commenmentioning

confidence: 97%

Impact of Microbiome on Ocular Health

2016

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The ocular surface is continuously exposed to the environment and, therefore, it is surprising that it harbors only few commensals with low degree of diversity. This unique aspect of the ocular surface physiology prompts the question whether there are core ocular commensal communities and how they affect ocular immunity. The purpose of this review is to provide an overview of what is known about the ocular surface commensals in health and disease and what we would like to learn in the near future. In addition, we discuss how microbiota at sites other than the eye may influence ocular immune responses. The information discussed in the review has been gathered using PubMed searches for literature published from January 1982 to December 2015.

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“…1 Keilty in 1930 2 used conventional bacterial culture methods and was among the first to enumerate the bacterial flora of the normal conjunctiva, and subsequent studies confirmed these findings. [3][4][5][6] These studies established that coagulase-negative Staphylococcus epidermidis, Propionibacterium sp., Corynebacterium sp., Staphylococcus aureus, Micrococcus sp., Pseudomonas sp., and Escherichia coli are associated with the ocular surface. It was also demonstrated that a good proportion of the conjunctiva (10%-43%) did not yield growth 6,7 and the diversity on the conjunctiva did not differ between the eyes by using culturing techniques.…”

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confidence: 95%

The Human Ocular Surface Fungal Microbiome

Shivaji

Jayasudha

Prashanthi

et al. 2019

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. To enumerate the ocular surface fungal microbiome of healthy human eyes by using next-generation sequencing (NGS). METHODS.Tarsal and fornix conjunctiva from the lower and upper lids of both eyes of healthy individuals were swabbed in duplicate separately. A total of 34 samples were collected from both the eyes of 17 individuals, which were used for the generation of ocular surface fungal microbiomes by NGS. Twenty-four swabs were used for the detection of culturable fungi by the conventional cultivable method. Microbiome generation involved DNA extraction, internal transcribed spacer 2 (ITS2) amplification, library preparation, amplicon sequencing, taxonomic assignment of sequences, diversity analyses, and identification of genera. RESULTS.The cultivable method detected fungi in 3 out of 24 (12.5 %) ocular surface swabs, whereas NGS identified fungi in 25 of the 34 (73.5 %) swabs. In the cultivable method Aspergillus was the only genus detected, whereas NGS detected 65 distinct genera with 12 to 24 genera per microbiome. Genera Aspergillus, Setosphaeria, Malassezia, and Haematonectria were present in the 25 eyes in which fungi were detected. Alpha diversity in the two eyes was similar and sex had no effect, but Chao1 and Simpson indices were altered by age.CONCLUSIONS. This study explored the ocular surface fungal microbiome of healthy individuals using NGS and identified a greater degree of diversity of fungi than with the conventional cultivable method. It was observed that several fungal genera were associated with the healthy conjunctiva.

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Lid and Conjunctival Micro Biota During Contact Lens Wear in Children

Cited by 39 publications

References 11 publications

Ocular surface microbiome in meibomian gland dysfunction

Ocular surface microbiome in meibomian gland dysfunction

Impact of Microbiome on Ocular Health

The Human Ocular Surface Fungal Microbiome

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