Ocular surface microbiome in meibomian gland dysfunction

Watters, Grant; Turnbull, Philip; Swift, Simon; Petty, Alex; Craig, Jennifer P.

doi:10.1111/ceo.12810

Cited by 72 publications

(51 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The need to understand the ocular microbiotome in health and disease, in specific patient populations and geographic locations, has been highlighted by this study and confers with the findings of prior investigations . The isolation of easy to culture gram positives by Watters et al is consistent with other studies utilizing conventional microbiological techniques . Molecular techniques, including next generation genome sequencing and bioinformatics, are now at the forefront of efforts to unravel the role of the ocular microbiotome in health and disease .…”

supporting

confidence: 81%

“…Currently lacking is data on temporal changes in the microbiotome; this information is needed to determine if the microbiota are responsive to changes in the host and environment. To build on the work of Watters et al future studies should utilize techniques able to identify non‐cultivable bacteria, distinguish viable from nonviable organisms, standardize inclusion criteria for patients and collect temporal data. Methods to address DNA contamination should also be considered as well as the influence of microbiota, at ocular and non‐ocular sites, on ocular immune responses …”

mentioning

confidence: 99%

“…4 It is therefore timely to consider if the 'ocular microbiotome' has a role in common ocular conditions such as meibomian gland disease. 3 Watters et al 5 present the findings of a prospective, cross-sectional and observational study of the ocular microbiotome in patients with meibomian gland disease seen in a university department in Auckland. They enrolled 157 patients, with mild (n = 41) or moderate-to-severe meibomian gland dysfunction (n = 50) and 66 normal patients.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Meibomian gland disease and the microbiotome: is it time for ocular probiotics?

Watson

2017

Clinical Exper Ophthalmology

View full text Add to dashboard Cite

supporting

confidence: 81%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Meibomian gland disease and the microbiotome: is it time for ocular probiotics?

Watson

2017

Clinical Exper Ophthalmology

View full text Add to dashboard Cite

“…6 Despite the low biomass issue, studies also attempted to study the microbial composition of other body sites for more direct linkage to the extraintestinal diseases. 7,8 As for ARC, the relationship between nasopharyngeal and ocular microbiome and the disease remained unexplored. In the present study, the nasopharyngeal and ocular microbiome from both healthy subjects and ARC patients were studied using 16S amplicon sequencing.…”

Section: Introductionmentioning

confidence: 99%

Characterization of ocular and nasopharyngeal microbiome in allergic rhinoconjunctivitis

Yau

Hou

Tsui

et al. 2019

Pediatric Allergy Immunology

View full text Add to dashboard Cite

Background Allergic rhinoconjunctivitis (ARC) is a prevalent allergic condition in the pediatric population. Microbial dysbiosis has increasingly been recognized to influence on host immunity and allergic diseases. However, the microbial profile of ARC has not been characterized. This cross‐sectional study aims to evaluate the changes in nasal and ocular surface microbiome of children with ARC. Methods Ocular and nasopharyngeal swabs were collected from controls and pediatric ARC cases for 16S rRNA amplicon sequencing. The bacterial community profile was analyzed. The correlation of the microbial diversity with the ARC‐related clinical scores was studied. Results A total of 23 patients with ARC and 17 healthy controls were recruited;30 were ocular samples (15 controls vs 15 ARC), while 40 were nasal samples (17controls vs 23 ARC) The alpha diversity of nasopharyngeal microbiome was significantly higher in ARC patients than healthy controls (P < 0.01), but not for ocular microbiome. The clinical scores in all subjects were negatively correlated with the Shannon diversity for ocular (P = 0.014) and positively correlated with nasopharyngeal (P = 0.010) microbiome. While the ocular microbiome remained significantly distinct from nasopharyngeal microbiome in terms of both alpha and beta diversity in both healthy subjects and ARC patients, significant differences of relative abundance of certain phyla (Bacteroidetes, Cyanobacteria, and Deinococcus‐Thermus) and genera (Dolosigranulum and Moraxella) between nasal and ocular surfaces were only detected in healthy controls, but not in the ARC subjects, suggesting the microbial composition at both body sites becoming more similar at disease state. Conclusion This study reported (a) a higher alpha diversity in ocular than nasopharyngeal microbiome in both ARC patients and controls, and (b) nasopharyngeal microbiome became more diverse in ARC patients than in controls. Our results suggested an interaction of the microbiome between ocular and nasal compartments in patients with ARC.

show abstract

“…The microbiome is altered in contact lens wear with observation of increased Gram‐negative bacteria . A recent study found that in contact lens wearers there were a higher proportion of sterile (no aerobic) counts resulting from cultures taken from the eyelid margins, before and after meibomian gland expression, compared with non‐lens wearers …”

Section: The Process Of Assessing the Tear Film And Ocular Surface Inmentioning

confidence: 99%

Tear film evaluation and management in soft contact lens wear: a systematic approach

Downie

Craig

2017

Clinical and Experimental Optometry

Self Cite

View full text Add to dashboard Cite

The human tear film is a highly ordered structure consisting of a thin layer of lipid on the surface and a thicker aqueous-mucin phase, which increases in mucin concentration toward the corneal epithelial cell layer. The health of the tear film and ocular surface influences the likelihood of being able to achieve successful contact lens wear. Contact lens discomfort and dryness are the most frequent reasons why contact lens wearers experience reduced wearing times, which can eventually lead to contact lens discontinuation. Comprehensive clinical assessment of tear film integrity and ocular surface health is therefore essential prior to commencing contact lens wear, to enable the ocular surface environment to be optimised to support lens wear. These parameters should also be evaluated over the course of contact lens wear, in order to identify any aspects requiring clinical management and ensure maintenance of optimal lens-wearing conditions. This review summarises current knowledge relating to the effects of soft contact lens wear on the tear film and ocular surface. It also provides a systematic approach to evaluating tear film and ocular surface integrity, in order to guide the clinical management of tear film anomalies with respect to contact lens wear.Key words: conjunctiva, contact lenses, cornea, dry eye, tearsThe tear film is a complex, precisely ordered fluid structure comprising a thin outer layer of lipid and a thicker underlying aqueous-mucin phase, with increasing mucin concentration toward the corneal epithelial cell layer. A thin layer of membrane-bound mucin, glycocalyx, imparts hydrophilicity to the hydrophobic corneal surface.1 In excess of 1,000 proteins or fractions of proteins have been identified within the human tear film.2,3 The tear fluid also contains a variety of regulated inflammatory mediators, including by-products of arachidonic acid metabolism, the complement cascade, cytokines and components derived from the ocular microbiome. 3,4The integrity of the tear film can strongly influence the likelihood of achieving successful contact lens wear. Contact lens discomfort and dryness are the most frequent reasons why contact lens wearers experience reduced wearing times, which can eventually lead to contact lens discontinuation. 5 Comprehensive clinical assessment of tear film quality and quantity, and ocular surface health are therefore essential both prior to commencing contact lens wear, to enable the ocular surface environment to be optimised to support lens wear, and over the course of lens wear, to identify any aspects requiring clinical attention to maintain optimal lens-wearing conditions. This review summarises current knowledge relating to the effects of soft contact lens wear on tear film composition, and outlines an approach to evaluating tear film and ocular surface integrity, to guide the clinical management of tear film anomalies with respect to contact lens wear. ASSESSING THE TEAR FILM AND OCULAR SURFACE IN CONTACT LENS WEAR: A SYSTEMATIC APPROACHUsing techniques such as in...

show abstract

Ocular surface microbiome in meibomian gland dysfunction

Abstract: Background: To investigate the ocular microbiome in meibomian gland dysfunction in Auckland, New Zealand.

Cited by 72 publications

References 37 publications

Meibomian gland disease and the microbiotome: is it time for ocular probiotics?

Meibomian gland disease and the microbiotome: is it time for ocular probiotics?

Characterization of ocular and nasopharyngeal microbiome in allergic rhinoconjunctivitis

Tear film evaluation and management in soft contact lens wear: a systematic approach

Contact Info

Product

Resources

About