Liang-Ge-San, a classic traditional Chinese medicine formula, attenuates acute inflammation in zebrafish and RAW 264.7 cells

Anti‑inflammatory activities of Gardenia jasminoides extracts in retinal pigment epithelial cells and zebrafish embryos

“…Previous reports have demonstrated that CuSO 4 at 20 µM causes primitive macrophage migration to the lateral line in zebrafish embryos (26)(27)(28). It was also found that the number of migrated macrophages in treated cells was significantly higher than that in untreated controls.…”

“…Breeding was set up in the Animal Unit at Glasgow Caledonian University using adult wild-type and transgenic zebrafish (Tg:zlyz-EGFP) expressing EGFP in primitive macrophages to get fertilized embryos, which were maintained in E3 medium consisting of 5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl 2 and 0.33 mM MgSO 4 (25). CuSO 4 has been used to induce acute inflammation, as previously described (26)(27)(28). A total of 24 transgenic zebrafish (Tg:zlyz-EGFP) embryos at 48 h post-fertilization (hpf) were treated with or without GJE (diluted in E3 medium, 5 µg/ml) for 24 h (i.e.…”

“…Additionally, the embryos were collected for further gene expression analysis. Lipopolysaccharides (LPS) have also been commonly used to induce inflammation in zebrafish (26)(27)(28)(29), and this method was also used in the present study. Wild-type zebrafish embryos at 48 hpf were treated with GJE (5 µg/ml) only, or with GJE plus LPS (5 µg/ml) for 24 h. Following the treatment, embryos were collected and subjected to gene expression analysis.…”

Screening of Bufadienolides from Toad Venom Identifies Gammabufotalin as a Potential Anti-inflammatory Agent

“…Our research group has successfully used these models in studying traditional Chinese medicines on inflammatory diseases [20,34]. Toad venom has been traditionally used for treating infectious and inflammatory diseases in China for thousands of years.…”

Anti-inflammatory, anti-angiogenetic and antiviral activities of dammarane-type triterpenoid saponins from the roots of Panax notoginseng

“…Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) are important pro-inflammatory cytokines in the inflammatory response that are initially released on exposure to LPS. 27 Therefore, the mRNA expression levels of IL-6 and TNF-α in LPS-injected larvae were measured using qRT-PCR to further confirm the anti-inflammatory effects of compound 11. As shown in Fig.…”