Leptospira idonii sp. nov., isolated from environmental water

Abstract

Strain Eri-1T was isolated from a water sample on the campus of Kyushu University, Fukuoka, Japan. The motility and morphology of the isolate were similar to those of members of the genus Leptospira , but the spiral structure of the isolate was sharper under dark-field microscopy. Cells were 10.6±1.3 µm long and 0.2 µm in diameter, with a wavelength of 0.9 µm and an amplitude of 0.4 µm. Strain Eri-1T grew in Korthof’s medium at both 13 and 30 °C, and also in the presence of 8-azaguanine. 16S rRNA gene-based phylogenetic analysis placed strain Eri-1T within the radiation of the genus Leptospira where it formed a unique lineage within the clade of the known saprophytic species of the genus Leptospira . The strain was not pathogenic to hamsters. Strain Eri-1T exhibited low levels (11.2–12.6 %) of similarity by DNA–DNA hybridization to the three most closely related species of the genus Leptospira . The DNA G+C content of the genome of strain Eri-1T was 42.5±0.1 mol%. These results suggest that strain Eri-1T represents a novel species of the genus Leptospira , for which the name Leptospira idonii sp. nov. is proposed. The type strain is Eri-1T ( = DSM 26084T = JCM 18486T).

High-Resolution Typing of Leptospira interrogans Strains by Multispacer Sequence Typing

“…is based on the expression of the lipopolysaccharide (LPS) antigens and has been used to define more than 300 serovars (1). The DNA-DNA hybridization data have been used to classify the genus Leptospira into 21 genomospecies, including saprophytic, intermediate, and pathogenic species (1,7,9,10).…”

A Review on Bovine Leptospirosis with Special Reference to Seroprevalence in India

“…Pathogenic Leptospira spp. classified into 28 serogroups and over 300 serovars based on LPS antigen (Saito et al, 2013).While the genetic homology in DNA hybridization have been identified 20 species of Leptospira spp. which includes 9 pathogenic, 5 Intermediate and 6 saprophytic species (Smythe et al, 2013).…”

Isolation and genetic detection of pathogenic Leptospira spp. from environmental soils and water in Central Luzon, Philippines

“…Aside from the animal host reservoir, the environment also serves as an important reservoir for pathogenic Leptospira [2]. Despite the important role of the environment in the transmission of Leptospira in human population, there are only few published studies on the prevalent Leptospira species present in the soil and water in the Philippines [4,5]. There was only one study on the isolation of leptospires from soils and water in the provinces of Central Luzon which was done in the province of Nueva Ecija.…”