Leptobacterium flavescens gen. nov., sp. nov., a marine member of the family Flavobacteriaceae, isolated from marine sponge and seawater

Mitra, Sharbanee; Matsuo, Yoshihide; Haga, Tomomi; Yasumoto-Hirose, Mina; Yoon, Jaewoo; Kasai, Hiroaki; Yokota, Akira

doi:10.1099/ijs.0.004358-0

Cited by 16 publications

(6 citation statements)

References 26 publications

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“…By applying a culture-dependent approach, we successfully isolated 151 strains from the sponge Ophlitaspongia sp., as well as their representative strains, which are distributed across 20 genera from 18 families in 4 phyla and include Actinobacteria , Bacteroidetes , Firmicutes , and Proteobacteria . Phylogenetic analyses confirmed that all 20 genera of the isolated strains overlapped with previously reported isolates associated with sponges and other marine hosts, such as corals, ascidians, and algae, from different geographical locations obtained using culture-dependent methods [ 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 ]. Genera such as Bacillus , Microbacterium , Nocardiopsis , Pseudoalteromonas , Saccharomonospora , and Streptomyces are distributed throughout aquatic environments and are well-known secondary metabolite producers that may protect host sponges from microbial infection and predators [ 47 , 48 , 49 , 50 , 51 , 52 ].…”

Section: Discussionsupporting

confidence: 80%

Antimicrobial Biosynthetic Potential and Phylogenetic Analysis of Culturable Bacteria Associated with the Sponge Ophlitaspongia sp. from the Yellow Sea, China

Chen

Wang

et al. 2022

Marine Drugs

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Sponge-derived bacteria are considered to be a promising source of novel drugs, owing to their abundant secondary metabolites that have diverse biological activities. In this study, we explored the antimicrobial biosynthetic potential and phylogenetics of culturable bacteria associated with the sponge Ophlitaspongia sp. from the Yellow Sea, China. Using culture-dependent methods, we obtained 151 bacterial strains, which were then analysed for their antimicrobial activities against seven indicator strains. The results indicate that 94 (62.3%) of the 151 isolated strains exhibited antimicrobial activities and inhibited at least one of the indicator strains. Fifty-two strains were selected for further phylogenetic analysis using 16S rRNA gene sequencing, as well as for the presence of polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) genes. These 52 strains belonged to 20 genera from 18 families in 4 phyla, including Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. Five strains with PKS genes and ten strains with NRPS genes were detected. Among them, two strains contained both PKS and NRPS genes. Notoacmeibacter sp. strain HMA008 (class Alphaproteobacteria) exhibited potent antimicrobial activity; thus, whole genome sequencing methods were used to analyse its secondary metabolite biosynthetic gene clusters. The genome of HMA008 contained 12 biosynthetic gene clusters that potentially encode secondary metabolites belonging to compound classes such as non-ribosomal peptides, prodigiosin, terpene, β-lactones, and siderophore, among others. This study indicates that the sponge Ophlitaspongia sp. harbours diverse bacterial strains with antimicrobial properties and may serve as a potential source of bioactive compounds.

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Section: Discussionsupporting

confidence: 80%

Antimicrobial Biosynthetic Potential and Phylogenetic Analysis of Culturable Bacteria Associated with the Sponge Ophlitaspongia sp. from the Yellow Sea, China

Chen

Wang

et al. 2022

Marine Drugs

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“…Cells of strain GJ16 T produced orange-coloured carotenoid-type pigments with absorption peaks at 451 nm, 474 nm (major peak) and 503 nm (Schmidt et al, 1994). The G+C content of the genomic DNA of strain GJ16 T was 39.4 mol%, a value between those reported for the two reference strains (Mitra et al, 2009;Liu et al, 2006).…”

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confidence: 47%

“…Strain GJ16 T formed a clade with the type strains of Leptobacterium flavescens (Mitra et al, 2009) and Zhouia amylolytica (Liu et al, 2006) with levels of 16S rRNA gene sequence similarity of 91.2 and 90.4 %, respectively (Fig. 1).…”

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confidence: 99%

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Gangjinia marincola gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae

Lee

Moon

Park

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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A novel strictly aerobic, orange-pigmented, Gram-stain-negative bacterium, designated strain GJ16T, was isolated from coastal seawater of Gangjin Bay, the southernmost part of the Korean peninsula, and subjected to a polyphasic taxonomic study. It grew optimally at 25–30 °C, at pH 7.0–8.0 and in the presence of 3 % NaCl. Comparative 16S rRNA gene sequence analysis revealed that strain GJ16T formed a distinct lineage within the family Flavobacteriaceae and shared less than 91.2 % 16S rRNA gene sequence similarity with members of the genera Leptobacterium, Zhouia, Winogradskyella, Dokdonia and Krokinobacter. The predominant cellular fatty acids were iso-C15 : 0 (40.2 %), iso-C15 : 1 G (12.8 %), iso-C17 : 0 3-OH (11.2 %) and C15 : 0 (6.6 %). The G+C content of the genomic DNA was 39.4 mol% and the major respiratory isoprenoid quinone was MK-6. On the basis of phenotypic and genotypic data, strain GJ16T represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Gangjinia marincola gen. nov., sp. nov. is proposed; the type strain is GJ16T (=KCTC 22649T =JCM 16082T).

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“…Strain SCSIO 03483 T formed a distinct branch and grouped with the clade including species from the genera Imtechella, Joostella and Zhouia, which was supported by a high bootstrap Table 1. Differential characteristics of strain SCSIO 03483 T and the type strains of related taxa in the family Flavobacteriaceae Taxa: 1, SCSIO 03483 T ; 2, Imtechella halotolerans K1 T (Surendra et al, 2012); 3, Joostella (J. marina En5 T and J. atrarenae M1-2 T ; Quan et al, 2008;Kim et al, 2011); 4, Zhouia amylolytica HN-171 T (Liu et al, 2006;Surendra et al, 2012); 5, Cellulophaga (C. fucicola NN015860 T , C. lytica DSM 7489 T and C. geojensis M-M6 T ; Johansen et al, 1999;Bowman, 2000;Nedashkovskaya et al, 2004); 6, Aquimarina muelleri KMM 6020 T (Nedashkovskaya et al, 2005a); 7, Leeuwenhoekiella (L. blandensis MED217 T and L. palythoae KMM 6264 T ; Nedashkovskaya et al, 2005b;Pinhassi et al, 2006;Nedashkovskaya et al, 2009); 8, Krokinobacter (K. genikus Cos-13 T , K. eikastus PMA-26 T and K. diaphorus MSKK-32 T ; Khan et al, 2006); 9, Leptobacterium flavescens YM3-301 T (Mitra et al, 2009); and 10, Lutaonella thermophila CC-MHSW-2 T (Arun et al, 2009) Cell size (mm) Length 4.0-4.6 1.2-1.8 0.8-2.0 1.3-3.0 1.2-4.7 5.0-7.0 1.4-3.2 2.5-4.0 8.5-9.0 1.0-2.0 Width 0.5-0.7 0.5-0.7 0.2-0.5 0.25-0.3 0.4-0.8 0.3-0.5 0.5-0.7 0.5-0.7 0.5-0.6 0.4-0. percentage in the neighbour-joining, maximum-likelihood and maximum-parsimony trees (Figs 1 and S1).…”

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Sinomicrobium oceani gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from marine sediment

Tian

Liu

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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A marine bacterium, designated SCSIO 03483T, was isolated from a marine sediment sample collected from the Nansha Islands in the South China Sea. The strain produced roundish colonies with diffusible yellow-coloured pigment on nutrient agar medium or marine agar 2216. Optimal growth occurred in the presence of 0–4 % (w/v) NaCl, at pH 7.0 and a temperature range of 28–37 °C. 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarity with Imtechella halotolerans K1T (92.7 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the isolate shared a lineage with members of the genera Imtechella , Joostella and Zhouia . Phospholipids were phosphatidylethanolamine, two unidentified aminolipids and three unknown polar lipids. The major respiratory quinone was MK-6 and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω6c/C16 : 1ω7c). The DNA G+C content of strain SCSIO 03483T was 38.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain SCSIO 03483T represents a novel species in a new genus in the family Flavobacteriaceae , for which the name Sinomicrobium oceani gen. nov., sp. nov. is proposed. The type strain of Sinobacterium oceani is SCSIO 03483T ( = KCTC 23994T = CGMCC 1.12145T).

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Leptobacterium flavescens gen. nov., sp. nov., a marine member of the family Flavobacteriaceae, isolated from marine sponge and seawater

Abstract: Leptobacterium flavescens gen. nov., sp. nov., a marine member of the family Flavobacteriaceae, isolated from marine sponge and seawater

Cited by 16 publications

References 26 publications

Antimicrobial Biosynthetic Potential and Phylogenetic Analysis of Culturable Bacteria Associated with the Sponge Ophlitaspongia sp. from the Yellow Sea, China

Antimicrobial Biosynthetic Potential and Phylogenetic Analysis of Culturable Bacteria Associated with the Sponge Ophlitaspongia sp. from the Yellow Sea, China

Gangjinia marincola gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae

Sinomicrobium oceani gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from marine sediment

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