Sinomicrobium oceani gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from marine sediment

Abstract

A marine bacterium, designated SCSIO 03483T, was isolated from a marine sediment sample collected from the Nansha Islands in the South China Sea. The strain produced roundish colonies with diffusible yellow-coloured pigment on nutrient agar medium or marine agar 2216. Optimal growth occurred in the presence of 0–4 % (w/v) NaCl, at pH 7.0 and a temperature range of 28–37 °C. 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarity with Imtechella halotolerans K1T (92.7 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the isolate shared a lineage with members of the genera Imtechella , Joostella and Zhouia . Phospholipids were phosphatidylethanolamine, two unidentified aminolipids and three unknown polar lipids. The major respiratory quinone was MK-6 and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω6c/C16 : 1ω7c). The DNA G+C content of strain SCSIO 03483T was 38.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain SCSIO 03483T represents a novel species in a new genus in the family Flavobacteriaceae , for which the name Sinomicrobium oceani gen. nov., sp. nov. is proposed. The type strain of Sinobacterium oceani is SCSIO 03483T ( = KCTC 23994T = CGMCC 1.12145T).

Sinomicrobium pectinilyticum sp. nov., a pectinase-producing bacterium isolated from alkaline and saline soil, and emended description of the genus Sinomicrobium

“…The description is as given by Xu et al (2013) with the following amendments. Catalase and oxidase activities are variable among species.…”

“…The DNA G+C content of the type strain, SCSIO 03483 T , of a single species of the genus, Sinomicrobium oceani, isolated from an abyssal sediment sample of the South China Sea, was 38.4 mol% (Xu et al, 2013). In addition to this species, many members of the family Flavobacteriaceae have been isolated from marine environments, such as seawater, sediments, sponge and tidal flat sediment.…”

“…The genus Sinomicrobium was proposed by Xu et al (2013) to include Gram-negative, aerobic, rod-shaped, non-sporeforming and motile (by gliding) marine bacteria that possess MK-6 as a major respiratory quinone and phosphatidylethanolamine as a main polar lipid. The DNA G+C content of the type strain, SCSIO 03483 T , of a single species of the genus, Sinomicrobium oceani, isolated from an abyssal sediment sample of the South China Sea, was 38.4 mol% (Xu et al, 2013).…”

Pustulibacterium marinum gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from the Bashi Channel

“…Preliminary sequence comparisons with 16S rRNA gene sequences deposited in the GenBank/EzTaxon-e (Kim et al, 2012) databases indicated that strain E403 T belonged to the family Flavobacteriaceae in the phylum Bacteroidetes. On the basis of 16S rRNA gene sequence similarity, type strains S. oceani SCSIO 03483 T (Xu et al, 2013), J. marina En5 T (Quan et al, 2008), Zhouia amylolytica HN-171 T (Liu et al, 2006) and Imtechella halotolerans K1 T (Surendra et al, 2012) shared 92.9, 92.4, 92.2 and 92.0 % sequence similarity with strain E403 T , respectively. In the neighbour-joining phylogenetic tree ( Fig.…”

“…The cultural, physiological and biochemical characteristics of strain E403 T are given in the species description and in Table 1. (Xu et al, 2013, and this study); 3, J. marina En5 T (Quan et al, 2008, and this study). ND, Not detected; 2, for fatty acids, less than 1 % of the total fatty acids or not detected; V, variable, the API 20NE results were different to those of Biolog.…”