Leptin receptor Q223R polymorphism in Egyptian female patients with breast cancer

Abstract

Aim of the studyBreast cancer is the most common cause of death in women. Obesity has been associated with increased risk of breast cancer in post-menopausal women. It induces chronic inflammation, which increases local and systemic levels of cytokines and adipokines such as leptin. Leptin (LEP) and leptin receptor (LEPR) genes have several polymorphisms in humans. This study aims to assess the association between blood levels of leptin and LEPR Q223R gene polymorphism in patients of cancer breast.Material and methodsThe current study was carried on 48 female breast cancer patients and 48 heathy female subjects. Carcinoembryonic antigen (CEA), cancer antibody CA15-3, and leptin hormone were determined. Single nucleotide polymorphism of LEPR Q223R was assessed by PCR/RFLP. Statistical analysis used: The statistical analysis of data was done by using SPSS version 20.ResultsThere were significant increases in the concentrations of CEA (p = 0.004), CA15-3 (p < 0.001), and leptin hormone (p < 0.001) in BC patients in relation to the respective concentrations in control subjects. CEA and CA 15-3 showed significant differences between various BC stages. As regard to LEPR Q223R gene polymorphism, AA genotype showed significantly higher frequency in BC patients when compared to their respective controls, with higher risk to develop BC.ConclusionsLeptin hormone shows significantly higher concentrations in BC patients. As regard to LEPR Q223R gene polymorphism, AA genotype showed significantly higher frequency in BC patients.

Association between serum leptin levels and breast cancer risk

“…[ 33 , 35 , 39 , 41 , 47 ] However, leptin levels have been shown to be inversely related to menopausal status in other studies. [ 8 , 11 , 13 , 16 – 18 , 24 , 31 , 34 , 40 , 44 ] Based on the results of our meta-analysis, we agree that leptin levels are associated with breast cancer among postmenopausal women but not among premenopausal women.…”

“…Seven of these 21 studies involved patients living in the United States, [ 14 , 34 – 39 ] 5 in Greece, [ 17 , 40 – 43 ] 2 in Mexico, [ 44 , 45 ] 2 in France, [ 18 , 46 ] 1 in Portugal, [ 8 ] 1 in Germany, [ 47 ] 1 in Norway, [ 48 ] 1 in Australia, [ 49 ] and 1 in Italy. [ 50 ] Two studies were conducted among Africans, 1 study was conducted in Egypt, [ 13 ] and 1 United States study included persons of African descent. [ 39 ] Among the 35 studies, 2 included only premenopausal women, [ 36 , 38 ] and 4 contained only postmenopausal women.…”

Investigation of TCF7L2, LEP and LEPR polymorphisms with esophageal squamous cell carcinomas

“…LEP is mainly secreted by adipose tissue, and has been suggested to promote tumor growth [ 30 ]. Some studies indicated that the serum LEP level was significantly higher in breast cancer patients compared with which in controls both pre-menopausal and post-menopausal [ 31 , 32 ]. A number of studies have found that LEP may play vital roles in cell proliferation, apoptosis, cell migration and angiogenesis [ 33 , 34 ].…”

Evaluation of Leptin Receptor Q223R (rs1137101) Polymorphism Based on Histopathological and Body Mass Index Characteristics in Iranian Females with Breast Cancer

“…Also, the risk of the disease was 3.704 times higher in patients with G allele than the ones with A allele, which indicates a strong association between this polymorphism and breast cancer. et al (21), as well as a meta-analysis by He et al (22) showed no significant correlation between this polymorphism and breast cancer. It seems that reasons such as sample size, employed techniques, smoking and alcohol consumption, environmental factors affecting the lifestyle, and genetic changes in different populations and races can affect the contradictory results.…”