Lentinan protects pancreatic β cells from STZ‐induced damage

Abstract

Pancreatic β‐cell death or dysfunction mediated by oxidative stress underlies the development and progression of diabetes mellitus (DM). In this study, we evaluated the effect of lentinan (LNT), an active ingredient purified from the bodies of Lentinus edodes, on pancreatic β‐cell apoptosis and dysfunction caused by streptozotocin (STZ) and the possible mechanisms implicated. The rat insulinoma cell line INS‐1 were pre‐treated with the indicated concentration of LNT for 30 min. and then incubated for 24 hrs with or without 0.5 mM STZ. We found that STZ treatment causes apoptosis of INS‐1 cells by enhancement of intracellular reactive oxygen species (ROS) accumulation, inducible nitric oxide synthase (iNOS) expression and nitric oxide release and activation of the c‐jun N‐terminal kinase (JNK) and p38 mitogen‐activated protein kinase (MAPK) signalling pathways. However, LNT significantly increased cell viability and effectively attenuated STZ‐induced ROS production, iNOS expression and nitric oxide release and the activation of JNK and p38 MAPK in a dose‐dependent manner in vitro. Moreover, LNT dose‐dependently prevented STZ‐induced inhibition of insulin synthesis by blocking the activation of nuclear factor kappa beta and increasing the level of Pdx‐1 in INS‐1 cells. Together these findings suggest that LNT could protect against pancreatic β‐cell apoptosis and dysfunction caused by STZ and therefore may be a potential pharmacological agent for preventing pancreatic β‐cell damage caused by oxidative stress associated with diabetes.

RETRACTED ARTICLE: Lentinan protects cardiomyocytes against hypoxia-induced injury by regulation of microRNA-22/Sirt1

“…The decreased ratio of Bax to Bcl-2 and suppressive cleavage of caspase-3 by LEN in hypoxia-treated cell indicated that LEN inhibited cell apoptosis to some extent. Our results are consistent with the previous study that LEN protects pancreatic b cells from streptozotocin-induced injury [26].…”

Adjuvant Therapy With Mushroom Polysaccharides for Diabetic Complications

“…The primary structure of Lentinan (400-800 kDa) contains five (1!3)-b-glucose residues in a linear linkage and two (1!6)-b-glucopyranoside side chains (Zhang et al, 2001). Lentinan can protect against pancreatic b-cell apoptosis and dysfunction by inhibiting ROS generation and exhibits antiapoptotic effects by preventing JNK and p38 MAPK signaling or/and antidysfunction effects by preventing NF-kB activation (Zhang et al, 2016). Krestin (94 kDa), also known as Coriolus versicolor polysaccharide, is a b-glucan-protein complex made up of approximate 25-38% protein residues.…”

Metabolomics insights into the modulatory effects of long-term compound polysaccharide intake in high-fat diet-induced obese rats

“…Lentinan, a well-known medicinal fungal polysaccharide, is a glucan produced by the edible mushroom Lentinus (or Lentinula ) edodes , and has been widely used as a component of traditional diets and medicine in Asia for hundreds of years [ 11 ]. Previous studies have shown that lentinan has hypolipidemic and hypoglycemic effects in cell culture experiments [ 12 , 13 ]. Flos Lonicera , another widely used medicinal and edible herb, also has hepatoprotective, antihypertensive, anti-inflammatory, antioxidative and antiviral activities [ 8 , 14 , 15 ], and Flos Lonicera polysaccharides (FLP) was reported to have a wide range of pharmacological effects, such as antioxidant and antiallergy effects [ 16 , 17 ].…”