Leishmania major strains isolated from distinct endemic areas show diverse cytokine mRNA expression levels in C57BL/6 mice: Toward selecting an ideal strain for the vaccine studies

Darabi, Sima; Khaze, Vahid; Riazi-Rad, Farhad; Darabi, Haiedeh; Bahrami, Fatemeh; Ajdary, Soheila; Alimohammadian, Mohammad Hossein

doi:10.1016/j.cyto.2015.05.022

Cited by 4 publications

(2 citation statements)

References 32 publications

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“…2,4,5,10 It could be explained by the huge pattern of virulence of L. major infective isolates already suggested by the great polymorphism of ZCL lesions and shown in mouse models and by genome sequencing of strains. [13][14][15][16] Several other factors such as the individual genetic susceptibility and immunity of the human host, 13 the number of inoculated promastigotes, 17 the sandfly's saliva composition, and the skin microbiota could also play a role. 18,19 Cutaneous leishmaniasis incubation times are interesting data that can be useful in routine practice.…”

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confidence: 99%

Assessment of Incubation Period of Cutaneous Leishmaniasis due to Leishmania major in Tunisia

Karim

Kalboussi

Sghaier

et al. 2020

The American Journal of Tropical Medicine and Hygiene

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The period between the infective sandfly bites and appearance of cutaneous leishmaniasis (CL) lesions is still hypothetical and little studied. This work aimed at assessing the incubation time of zoonotic CL (ZCL) due to Leishmania major using a standardized methodology. The retrospective analysis used the epidemiological, clinical, and biological information available in the database recording all the CL cases diagnosed at the Parasitology Department of the Pasteur Institute of Tunis during 2015-2019. It allowed for the selection of 92 privileged observations 1) of confirmed CL cases with presentation suggestive of ZCL form 2) living in northern regions free of ZCL 3) with a single infective trip of less than a week to ZCL foci during transmission season and 4) with accurate dates of travel and onset of lesions. Incubation length computed in this population ranged from 1 to 21 weeks, with a median of 5 weeks (interquartile range: 3-8.5 weeks).

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confidence: 99%

Assessment of Incubation Period of Cutaneous Leishmaniasis due to Leishmania major in Tunisia

Karim

Kalboussi

Sghaier

et al. 2020

The American Journal of Tropical Medicine and Hygiene

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“…A leishmaniose visceral é uma zoonose altamente preocupante em virtude dos sinais clínicos e gravidade variável que pode provocar, além do grande número de indivíduos suscetíveis (Gontijo e Melo, 2004;Diaz et al, 2012). Muitas estratégias têm sido adotadas a fim de se produzir imunobiológicos com capacidade de estimular respostas eficientes e duradouras frente ao parasito causador da doença (Thakur et al, 2015;Darabi et al, 2015;Martins et al, 2016). No entanto, as complexas interações estabelecidas entre Leishmania spp.…”

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O probiótico Saccharomyces boulardii modula a resposta de IgG2a em camundongos expostos aos antígenos de Leishmania infantum

Lima

Sampaio²,

Silva³

et al. 2019

Revista Brasileira De Ciência Veterinária

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O objetivo do presente estudo foi avaliar os efeitos do probiótico Saccharomyces boulardii na modulação da resposta imune humoral de animais expostos a antígenos de Leishmania infantum. Para isso, 16 camundongos BALB/c foram imunizados com antígeno particulado de Leishmania infantum e divididos em dois grupos experimentais, um composto por animais suplementados e outro por animais não suplementados com o probiótico. Amostras de sangue dos animais foram colhidas semanalmente durante o período experimental e submetidas ao Ensaio da Imunoabsorbância Ligado à Enzima indireto para avaliação dos títulos de IgG totais e o perfil dos isotipos de IgG produzidos (IgG1 e IgG2a). A suplementação com o probiótico não exacerbou a produção de IgG total em comparação ao grupo controle, não havendo diferenças significativas entre os dois grupos. Porém, as soroconversões de IgG2a foram mais elevadas no grupo suplementado, no qual registrou-se um aumento de 1,46 vezes no final do experimento. Assim, a suplementação com S. boulardii foi capaz de modular a resposta de IgG2a/IgG1 nos animais expostos aos antígenos de Leishmania infantum.

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Behavior of two Leishmania infantum strains—evaluation of susceptibility to antimonials and expression of microRNAs in experimentally infected J774 macrophages and in BALB/c mice

Silva

Almeida

et al. 2018

Parasitol Res

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Strains of the same Leishmania parasite species, isolated from different host organisms, may exhibit unique infection profiles and induce a change in the expression of microRNAs among host macrophages and in model host mice. MicroRNAs (MiR) are endogenous molecules of about 22 nucleotides that are involved in many regulatory processes, including the vertebrate host immune response. In this respect, the infectivity and susceptibility to antimonials of two L. infantum strains, BH46, isolated from human, and OP46, isolated from symptomatic dog, were characterized in J774 macrophages and BALB/c mice. Parasite burden was assessed in the liver, spleen, and bone marrow using the serial limiting dilution technique. A higher parasite burden was observed in the spleen and bone marrow of animals infected with OP46 compared to BH46 strain. Our results also showed that OP46 was less susceptible to the antimonials. In addition, miR-122 and miR-155 expression was evaluated in the liver and J774 macrophages, and in spleens from infected animals, respectively. An increase was observed in the expression of miR-155 in J774 macrophages infected with both strains compared to uninfected cells, with a higher expression in cells infected with OP46. However, no difference in the expression of miR-122 and miR-155 was observed in the infected animals. Thus, this study shows that OP46 was more infective for mice, it caused a higher increase in miR-155 expression in infected macrophages and was less susceptible to the antimonials evaluated. These data suggest that alteration in miR-155 level likely plays a role in regulating the response to L. infantum.

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Leishmania major strains isolated from distinct endemic areas show diverse cytokine mRNA expression levels in C57BL/6 mice: Toward selecting an ideal strain for the vaccine studies

Cited by 4 publications

References 32 publications

Assessment of Incubation Period of Cutaneous Leishmaniasis due to Leishmania major in Tunisia

Assessment of Incubation Period of Cutaneous Leishmaniasis due to Leishmania major in Tunisia

O probiótico Saccharomyces boulardii modula a resposta de IgG2a em camundongos expostos aos antígenos de Leishmania infantum

Behavior of two Leishmania infantum strains—evaluation of susceptibility to antimonials and expression of microRNAs in experimentally infected J774 macrophages and in BALB/c mice

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