Leishmania braziliensis: Cell surface differences in promastigotes of pathogenic and nonpathogenic strains

Ayesta, C.; Argüello, Carlos; Hernández, Ángel González

doi:10.1016/0014-4894(85)90071-2

Cited by 16 publications

(6 citation statements)

References 9 publications

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“…Leishmania promastigotes and amastigotes are known to have a negative surface charge 25‐29 . Charge‐related interactions between WBCs and filter fibers have been proposed as a possible mechanism for the removal of WBCs by the filter.…”

Section: Discussionmentioning

confidence: 99%

Leukodepletion filters reduce Leishmania in blood products when used at collection or at the bedside

Cardo

Salata²,

Harman³

et al. 2006

Transfusion

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Section: Discussionmentioning

confidence: 99%

Leukodepletion filters reduce Leishmania in blood products when used at collection or at the bedside

Cardo

Salata²,

Harman³

et al. 2006

Transfusion

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“…Promastigote and amastigote forms of some species of Leishmania also presented negative surface charge detected by cationic particles (Mühlp-fordt 1975, Pimenta and De Souza 1983, Ayesta et al 1985, Saraiva et al 1989.…”

Section: Ultrastructural Detection Of Cell Surface Anionic Sitesmentioning

confidence: 99%

“…Cationized ferritin binds to the surface of promastigotes of L. donovani, L. tropica and L. braziliensis (Mühlpfordt 1975, Dwyer 1977, Ayesta et al 1985. In L. braziliensis CF binding was used to identify promastigotes, which belong to pathogenic and non-pathogenic strains.…”

Section: Ultrastructural Detection Of Cell Surface Anionic Sitesmentioning

confidence: 99%

“…In L. braziliensis CF binding was used to identify promastigotes, which belong to pathogenic and non-pathogenic strains. Only pathogenic promastigotes were strongly agglutinated by CF and presented an abundant CF surface labeling (Ayesta et al 1985). Cationized ferritin and colloidal iron hydroxide bind to the surface of amastigote and promastigote forms of L. mexicana amazonensis (Pimenta and De Souza 1983).…”

Section: Ultrastructural Detection Of Cell Surface Anionic Sitesmentioning

confidence: 99%

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The surface charge of trypanosomatids

Souto-Padrón

2002

An. Acad. Bras. Ciênc.

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The surface charge of trypanosomatids was evaluated by means of the binding of cationic particles, as visualized by electron microscopy and by direct measurements of the electrophoretic mobility of cells. The results obtained indicate that most of the trypanosomatids exhibit a negatively charged surface whose value is species specific and varies according to the developmental stages. Sialic acids associated with glycoproteins, glycolipids and phosphate groups are the major components responsible for the net negative surface charge of the trypanosomatids.

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“…The human sera were tested against two representative strains of Leishmania (Barker & Butcher 1983, Lainson 1983: L. mexicana mexicana (7369) and L. braziliensis braziliensis (2903), kindly provided by Dr L. Yarzabal, Instituto Nacional de Dermatologia, Caracas, and obtained originally from Dr R. Shaw, Instituto Evandro Chagas de Belem, Para, Brazil. Further studies of specificity were performed using L. braziliensis guyanensis (7937), strain NR (tentatively classified as L. braziliensis; Ayesta, Arguello & Hernandez 1985); L. mexicanapifanoi (7940), strains AMP (Perez, Labrador & Torrealba 1979) and LR (isolated from diffuse cutanous leishmaniasis and considered as L. mexicana) and strain HM ( L . garnhami or L. mexicana garnhami; Scorza et al 1979, Barker & Butcher 1983, Lainson 1983.…”

Section: Leishmania Strainsmentioning

confidence: 99%

IgE antibody against surface antigens of Leishmania promastigotes in American cutaneous leishmaniasis

Lynch

Malavé

Turner

et al. 1986

Parasite Immunology

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As IgE-mediated immune mechanisms participate in the host defence against some types of parasites, we evaluated sera from American cutaneous leishmaniasis (ACL) patients for the presence of this antibody against Leishmania. Using monoclonal antibodies against human IgE and an immunoperoxidase staining technique, 48% of the patients sera tested were found to contain IgE antibody that bound strongly to Leishmania promastigotes. A much lower proportion of sera from non-symptomatic subjects from either endemic or non-endemic areas of the disease contained significant levels of anti-Leishmania IgE antibody (6.5% and 0% respectively). The results indicated that the IgE antibody bound predominantly to surface components of the promastigotes, and reactivity against the intracellular amastigote form of the parasite was rarely detected. Somewhat unexpectedly, in a small proportion of the sera, the IgE antibody showed apparent specificity for L. mexicana or L. braziliensis. This study demonstrates that ACL patients can develop anti-Leishmania IgE antibody responses, that seem to be directed preferentially against surface antigens of promastigotes, and that can be strain specific. This raises the question as to the possible contribution of this antibody to the immune defence mechanisms against the parasite.

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Leishmania braziliensis: Cell surface differences in promastigotes of pathogenic and nonpathogenic strains

Cited by 16 publications

References 9 publications

Leukodepletion filters reduce Leishmania in blood products when used at collection or at the bedside

Leukodepletion filters reduce Leishmania in blood products when used at collection or at the bedside

The surface charge of trypanosomatids

IgE antibody against surface antigens of Leishmania promastigotes in American cutaneous leishmaniasis

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