“…Data on validity, feasibility, and reproducibility of LASF have been previously reported by our group in several articles. 4,5,18,19 Transmitral and pulmonary vein pulsed-wave Doppler curves and early diastolic tissue Doppler velocity of mitral annulus (E 0 ) were assessed according to the recommendations of the American Society of Echocardiography. 20 Early diastolic velocity of transmitral flow (E) was divided by E 0 and used to classify LV diastolic function together with other parameters (E/A ratio of transmitral flow, deceleration time of E, and the difference of atrial wave duration on pulmonary vein flow minus atrial wave duration on transmitral flow in 4°as proposed by Redfield et al 21 ): normal diastolic function, mild, moderate, and severe diastolic dysfunction.…”