LEF1-AS1 contributes to proliferation and invasion through regulating miR-544a/ FOXP1 axis in lung cancer

Abstract

SummaryLong non-coding RNAs (lncRNAs) are increasingly recognized as important regulators in tumor development. This study aims to investigate the potential role oflncRNALEF1-AS1, in the progression of lung cancer. Quantitative real-time PCR (qRT-PCR) and western blot assays showed that LEF1-AS1 was upregulated while miR-544a was downregulated in lung cancer specimens and cells. Overexpression of LEF1-AS1 led to the enhancement of cell proliferation and invasion, revealed by CCK-8 assay and transwell assay. A negative correlation was found between LEF1-AS1 and miR-544a. BLAST analysis and dual-luciferase assay confirmed that FOXP1 is a downstream effector of miR-544a. Therefore, the LEF1-AS1/miR-544a/FOXP1 axis is an important contributor to lung cancer progression. Collectively, our novel data uncovers a new mechanism that governs tumor progression in lung cancer and provides new targets that may be used for disease monitoring and therapeutic intervention of lung cancer.

LncRNA LEF1-AS1/LEF1/FUT8 Axis Mediates Colorectal Cancer Progression by Regulating α1, 6-Fucosylation via Wnt/β-Catenin Pathway

“…Lymphoid enhancer-binding factor 1 (LEF1) antisense RNA 1 (LEF1-AS1), is a highly conserved transcript and a newfound lncRNA encode in the plus strand of LEF1 at chromosome 4q25, which is rstly found to be upregulated and predict prognosis in CRC [6]. In recent years, the distinct upregulation of LEF1-AS1 was demonstrated in several tumors, such as glioblastoma, oral squamous cell carcinoma, lung cancer and prostate cancer [7][8][9][10]. However, LEF1-AS1 is highly expressed in normal hematopoietic stem cells but barely detectable in myeloid malignant cell lines [11].…”

Risk variant in miR-544a binding site in CCDC170 is associated with osteoporosis

“…MicroRNAs influence posttranscriptional regulation in various genes, and some have been shown to influence osteoporosis[20,21]. MiR-544a has been shown to play an important role in many diseases such as lung cancer and breast cancer, but its role in osteoporosis is unknown[22,23]. Here, we showed that miR-544a suppressed osteogenesis and promoted osteoclastogenesis by downregulating the expressions of RUNX2 , Osterix , ALP, Col1a1 , OPN , OCN and OPG .…”

<p>LncRNA LEF1-AS1 Promotes Ovarian Cancer Development Through Interacting with miR-1285-3p</p>

“…15 Furthermore, it was demonstrated that LEF1-AS1 regulates growth and migration of lung cancer cells by sponging miR-544a and enhancing FOXP1 translation. 26 LEF1-AS1 was also demonstrated to regulate proliferation and metastasis of oral squamous cell carcinoma and myeloid tumor. 16,27 Thus, LEF1-AS1 is a very important lncRNA involved in tumorigenesis.…”

“…13 A number of experiments have confirmed that LEF1-AS1 is a sponge for several miRNAs, including miR-330-5p, miR-544a and miR-489. 15,17,26 LncRNA could directly interact with miRNAs to suppress their levels, consequently inducing upregulation of the miRNA downstream genes. 29 For instance, LINC01116 interacts with miR-136 to promote FN1 expression in oral squamous cell carcinoma.…”