Lead-Induced Damage on Hepatocytes and Hepatic Reticular Fibres in Rats; Protective Role of Aqueous Extract of <i>Moringa oleifera</i> Leaves (Lam)

Abstract

This study was designed to evaluate the hepatoprotective and ameliorative effects of aqueous extract of Moringa oleifera (MO) leaves on the histologyof liver and hepatic reticular fibres integrity of adult Wistar rats following lead-induced hepatotoxicity. Twenty four adult Wistar rats, weighing 180-220 g, randomly assigned into four groups of six animals each were used for the study. Lead and Moringa oleifera were given orally to the rats. 24 hours after the last administration, animals were sacrificed, blood obtained by cardiac puncture and liver excised, fixed in 10% phosphate buffered formalin for histological and histochemical analysis. The activities of aspartate amino transferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) were used as markers of hepatotoxicity and catalase (CAT) activity were used as marker to evaluate the anti-oxidant status of the tissue. Result showed that lead treatment increased markers of hepatic damage (ALT, AST and ALP) and decreased CAT activities. Histological studies reveal alterations of hepatic structure including hepatocytic vacuolations, sinuosoidal congestion and loss of reticular fibres following lead treatment. Treatment with MO prevented and reversed lead induced hepatic damage. In conclusion, this study shows that Moringa oleifera leaf extract has an appreciable ability to prevent hepatotoxicity caused by lead, partly as result of its chemical constituents which has hepatoprotective properties.

In-vitro and in-vivo determination of hydroxyl radical scavenging activity (hrsa) of fractions of aqueous extract of moringa oleifera leaves (aemol)

“…Significant increase in Catalase activity in fractions of AE-MOL treated group is in accordance with the findings of Omotoso et al, [18] who reported administration of AEMOL with lead afford significant increase in catalase activitiy.…”

A digital image analysis study on the disintegration kinetics of reticular fibers in the ethylene glycol‐induced rat liver tissue

“…Due to the regulation of reticular fibers (reticulin), it has a branched and network‐like structure, often called a reticulum. These fibers are necessarily type III collagen fibrils (Jobson et al, 2015; Omotoso, Abiodun, Ijomone, & Adewole, 2015; Wiki, 2020).…”

Effect of Moringa Oleifera Leaves on Bisphenol-A Induced Histological Changes of Hepatocytes in Albino Rats

“…This is also considered as "Miracle tree" and its leaves hold hepatoprotective, anti-inflammatory, antihypertensive and antimicrobial activities 9 . The leaves of this miraculous plant are rich in antioxidants (SOD and Catalase) phenols, flavonoids and caretenoids 10 , which imparts to its hepatoprotective potential Therapy with Mo leaves reduces the liver damage and promotes regeneration 11 , this may be associated with the preventive and preserving potential of Moringa oleifera on plasmalemma and increased protein synthesis. These leaves possess combination of calcium, iron, protein, carbohydrates, copper and vitamins are helpful in lowering blood pressure and glucose levels and exhibit anti-ulcer and anti-inflammatory effect.…”