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Sensing dynamic cytoplasm refractive index changes of adherent cells with quantitative phase microscopy using incorporated microspheres as optical probes

“…As no modification of the experimental setup is required, the measurement principle prospects to be used with several existing quantitative phase contrast imaging techniques, [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] and the usage in multi-modal imaging platforms 53 is simplified. Finally, as many cells internalize particles by phagocytic behavior, the method may be applicable with a variety of different cell types.…”

Review of spectral imaging technology in biomedical engineering: achievements and challenges

“…139,140 Then, Wachman et al developed an AOTF microscope to map hemoglobin saturation and oxygen tension in mouse brains. 99,100 Since then, more AOTF-based microscopy HSI systems have been proposed and used for different biological analysis, for example, the HSI system, 86 the molecular HSI (MHSI) system, 96 the HSI microscope, 141 etc. Besides microscope, endoscope and camera lens also have been attached to AOTF to detect biological tissues in vivo.…”

Mapping lipid and collagen by multispectral photoacoustic imaging of chemical bond vibration

“…1 Recently this method has been brought to the imaging field to enable label-free detection of lipids and water in tissues or tissue phantoms. [2][3][4][5][6][7] Photoacoustic imaging using vibrational overtone absorption as a contrast mechanism is appealing because of the following advantages: 1. The signal arises from the absorption of specific chemical bonds, thus providing chemical selectivity; 2. by excitation at specific near-infrared (NIR) regions, where blood absorption and tissue scattering are reduced and water absorption has a local minimum, millimeter-to-centimeter scale tissue penetration can be reached; 3. tissue damage is significantly reduced by applying excitation at NIR regions, so that higher pulse energy can be used to compensate the relatively low cross-section of overtone absorption.…”

“…2,[4][5][6] More studies show that by utilizing excitation at 1730 nm, where the first overtone of C-H vibration is located, photoacoustic signal of lipid is enhanced compared to that with 1210-nm excitation. 1,3,8 In this study, we further explore the capability of photoacoustic imaging by taking advantage of the spectral information embedded in a vibrational overtone spectrum. In our previous study, CH 2 and CH 3 groups were shown to have distinctive spectral profiles.…”

“…In our previous study, CH 2 and CH 3 groups were shown to have distinctive spectral profiles. 3 Those distinctive features offer exciting opportunities to distinguish lipids and collagen that are rich in CH 2 and CH 3 groups, respectively. This area of study is important because lipid deposition and collagen remodeling occur in many kinds of human diseases, including atherosclerosis and fatty liver diseases.…”