Mapping lipid and collagen by multispectral photoacoustic imaging of chemical bond vibration

Wang, Pu; Wang, Ping; Wang, Hanwei; Cheng, Ji‐Xin

doi:10.1117/1.jbo.17.9.096010

Cited by 57 publications

(69 citation statements)

References 29 publications

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“…For lipids, the absorption peaks at 920, 1040, 1210, 1730, and 1760 nm are associated with overtones of the stretching vibrational mode of the C-H bond. 9,10 The 920 and 1210 nm peaks are associated with the second overtone of C-H stretching, 10,11 while the 1730 and 1760 nm peaks are associated with the first overtone of C-H stretching. [10][11][12] The absorption peak near 1430 nm can be attributed to the first overtone of O-H stretching.…”

Section: Introductionmentioning

confidence: 99%

“…9,10 The 920 and 1210 nm peaks are associated with the second overtone of C-H stretching, 10,11 while the 1730 and 1760 nm peaks are associated with the first overtone of C-H stretching. [10][11][12] The absorption peak near 1430 nm can be attributed to the first overtone of O-H stretching. 10,12 Different types of lipids, such as cholesterol, cholesterol esters, phospholipids, and triglycerides, are known to have absorption spectra that are similar to each other, with distinctive peaks in the SWIR region.…”

Section: Introductionmentioning

confidence: 99%

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Review of short-wave infrared spectroscopy and imaging methods for biological tissue characterization

et al. 2015

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Review of short-wave infrared spectroscopy and imaging methods for biological tissue characterization

et al. 2015

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“…The selection of wavelengths may be further optimized to improve sensitivity. Alternatively, sensitivity might be increased by imaging in the 1700 nm wavelength range, where lipid absorption is much higher [18,26]. Tradeoffs exist in the form of a high water absorption, which may present a challenge in this wavelength range, and the chemical specificity of the absorption spectra of the different lipids, which remains to be explored.…”

Section: Discussionmentioning

confidence: 99%

“…4 shows a healthy artery, which by IVPA is characterized by an absence of lipid signal in the intima and media. Other methods have been reported for separating lipids from other tissue types [26], relying on the acquisition of more wavelengths. This richer spectral sampling may increase robustness against measurement noise, permits automated spectral unmixing [27], and possibly enhances chemical detail.…”

Section: Discussionmentioning

confidence: 99%

Lipid detection in atherosclerotic human coronaries by spectroscopic intravascular photoacoustic imaging

Jansen¹,

Wu²,

Steen³

et al. 2013

Opt. Express

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Abstract:The presence of lipids in atherosclerotic coronary lesions is an important determinant of their potential to trigger acute coronary events. Spectroscopic intravascular photoacoustic imaging (sIVPA) has the potential to automatically detect lipids in atherosclerotic lesions. For realtime in vivo imaging, limiting the number of excitation wavelengths is crucial. We explored methods for plaque lipid detection using sIVPA, with the aim to minimize the number of laser pulses per image line. A combined intravascular ultrasound (IVUS) and photoacoustic imaging system was used to image a vessel phantom and human coronary arteries ex vivo. We acquired co-registered cross-sectional images at several wavelengths near 1200 nm, a lipid-specific absorption band. Correlating the photoacoustic spectra at 6 or 3 wavelengths from 1185 to 1235 nm with the absorption spectrum of cholesterol and peri-adventitial tissue, we could detect and differentiate the lipids in the atherosclerotic plaque and peri-adventitial lipids, respectively. With two wavelengths, both plaque and peri-adventitial lipids were detected but could not be distinguished. Raizner, and G. L. Kaluza, "In vivo plaque characterization using intravascular ultrasound-virtual histology in a porcine model of complex coronary lesions," Arterioscler. Thromb.

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“…18 In principle, PAT can be used to image and quantify the concentration of any molecule, based on its absorption spectrum. For instance, PAT has been used to image oxy-and deoxyhemoglobin, 21,32-34 melanin, 35,36 water, 37,38 lipids, [39][40][41] DNA and RNA, [42][43][44] and cytochromes. 45,46 Figure 2(a) indicates that hemoglobin is the most optically absorbing contrast for wavelengths below 1000 nm.…”

Section: Label-free Photoacoustic Imagingmentioning

confidence: 99%

Label-free photoacoustic tomography of whole mouse brain structures ex vivo

Xia

et al. 2016

Neurophoton.

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Abstract. Capitalizing on endogenous hemoglobin contrast, photoacoustic-computed tomography (PACT), a deep-tissue high-resolution imaging modality, has drawn increasing interest in neuroimaging. However, most existing studies are limited to functional imaging on the cortical surface and the deep brain structural imaging capability of PACT has never been demonstrated. Here, we explicitly studied the limiting factors of deep brain PACT imaging. We found that the skull distorted the acoustic signal and blood suppressed the structural contrast from other chromophores. When the two effects are mitigated, PACT can potentially provide high-resolution label-free imaging of structures in the entire mouse brain. With 100-μm in-plane resolution, we can clearly identify major structures of the brain, which complements magnetic resonance microscopy for imaging small-animal brain structures. Spectral PACT studies indicate that structural contrasts mainly originate from cytochrome distribution and that the presence of lipid sharpens the image contrast; brain histology results provide further validation. The feasibility of imaging the structure of the brain in vivo is also discussed. Our results demonstrate that PACT is a promising modality for both structural and functional brain imaging.

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Mapping lipid and collagen by multispectral photoacoustic imaging of chemical bond vibration

Cited by 57 publications

References 29 publications

Review of short-wave infrared spectroscopy and imaging methods for biological tissue characterization

Review of short-wave infrared spectroscopy and imaging methods for biological tissue characterization

Lipid detection in atherosclerotic human coronaries by spectroscopic intravascular photoacoustic imaging

Label-free photoacoustic tomography of whole mouse brain structures ex vivo

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