Abstract:This observational study was carried out as a preliminary one to see the effectiveness and reliability of an easy noninvasive approach for the confirmatory diagnosis of Visceral Leishmaniasis (VL) by detection of amastigote forms (LD bodies) of the parasite. Clinically suspected 73 febrile cases were investigated by examination of bone marrow/splenic aspirate to detect LD bodies, of whom 57 (41.61%) showed LD positive result on microscopy and/or culture in modified Novy, Nichole, MacNeal (NNN) medium. Blood bu… Show more
“…Diagnosis of VL, mainly by splenic/bone marrow aspiration is considered as gold standard. Splenic aspiration is more sensitive (85-90%) as compared to bone marrow aspiration (50-60%) 16 . However, these days PCR/rt-PCR with splenic aspiration can be more sensitive and specific.…”
“…Diagnosis of VL, mainly by splenic/bone marrow aspiration is considered as gold standard. Splenic aspiration is more sensitive (85-90%) as compared to bone marrow aspiration (50-60%) 16 . However, these days PCR/rt-PCR with splenic aspiration can be more sensitive and specific.…”
“…The sensitivity of buffy smear was found to be 37.8% which is in consistent with results reached by Diro et al (2017) who reported a sensitivity of 19.5% and Rohrs (1964) a sensitivity of 35%. Shamsuzzaman et al (2007) used the method of density gradient centrifugation using mono-nuclear cell separation fluid "Lymphoprep"and the sensitivity raised to 92.98%. This low percentage could be attributed to the method used for the collection of the buffy coat layer from the blood.…”
Visceral Leishmaniasis is considered by WHO as one of the leading diseases in the world and is present in 88 countries worldwide.It affects poor communities in peripheral areas. It infects the internal organs of the body. It is fatal if not treated.It is caused by the parasite L.donovani in the old world and L.infantum in the new world. Correct diagnosis is necessary if the disease is to be treated.Many diagnostic techniques are known but none is agreed upon worldwide.This study aims to compare two techniques for the diagnosis of VL in Sudan.37 patients was included in this study.Buffy coat smears were prepared from peripheral blood of these patients and were searched for the presence of the parasite.Serum from these blood samples was used to preform DAT technique.The sensitivity of buffy coat smear was 37.8% and that of DAT was 100%.When MacNemar test was conducted, it was found that DAT is better than buffy coat smear for the diagnosis of VL.We concluded that DAT is a better diagnostic technique and could be adapted for diagnosis of VL in Sudan.
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